Circulating MicroRNAs do not provide a diagnostic benefit over
tissue biopsy in patients with brain metastases

J 2025

Circulating MicroRNAs do not provide a diagnostic benefit over tissue biopsy in patients with brain metastases

RUČKOVÁ, Michaela; Dagmar AL TUKMACHI; Marek VEČEŘA; Tereza DEISSOVÁ; Robin JUGAS et al.

Základní údaje

Originální název

Circulating MicroRNAs do not provide a diagnostic benefit over tissue biopsy in patients with brain metastases

Autoři

Vydání

Nature Scientific Reports, Berlin, NATURE RESEARCH, 2025, 2045-2322

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30204 Oncology

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.900 v roce 2024

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/25:00143790

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

Brain metastases; MicroRNAs; Liquid biopsies; Diagnostics; Small RNA sequencing

Štítky

14110112, 14110131, 14110224, 14110230, 14110513, 14313050

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 25. 2. 2026 14:18, Mgr. Tereza Miškechová

Anotace

V originále

Brain metastases (BMs) are frequent and devastating complications of systemic malignancies, necessitating accurate diagnosis and origin identification for effective treatment strategies. Invasive biopsies are currently required for definitive diagnosis, highlighting the need for less invasive diagnostic approaches and robust biomarkers. Circulating microRNAs (miRNAs) have demonstrated potential as sensitive and specific diagnostic biomarkers in various cancers. Thus, our objective was to identify and compare miRNA profiles in BM tissue, cerebrospinal fluid (CSF), and plasma, with a specific focus on liquid biopsies for diagnostic purposes. Total RNA enriched for miRNAs was isolated from histopathologically confirmed BM tissues (n = 30), corresponding plasma samples (n = 30), and CSF samples (n = 27) obtained from patients with diverse BM types. Small RNA sequencing was employed for miRNA expression profiling. Significantly differentially expressed miRNAs were observed in BM tissues, enabling the differentiation of primary origins, particularly breast, colorectal, renal cell carcinoma, and melanoma metastases. The heterogeneity observed in lung carcinomas also manifested in the corresponding BMs, posing challenges in accurate discrimination from other BMs. While tissue-specific miRNA signatures exhibited the highest precision, our findings suggest low diagnostic potential of circulating miRNAs in CSF and blood plasma for BM patients. Our study represents the first analysis of miRNA expression/levels in a unique set of three biological materials (tissue, blood plasma, CSF) obtained from the same BM patients using small RNA sequencing. The presented results underscore the importance of investigating aberrant miRNA expression/levels in BMs and highlight the low diagnostic utility of circulating miRNAs in patients with BMs.

Návaznosti

LM2018132, projekt VaV

Název: Národní centrum lékařské genomiky (Akronym: NCLG)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Národní centrum lékařské genomiky

LX22NPO5102, projekt VaV

Název: Národní ústav pro výzkum rakoviny (Akronym: NÚVR)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Národní ústav pro výzkum rakoviny, 5.1 EXCELES

LX22NPO5107, projekt VaV

Název: Národní ústav pro neurologický výzkum

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Národní ústav pro neurologický výzkum, 5.1 EXCELES

NV18-03-00398, projekt VaV

Název: Rozšíření současných prognostických skórovacích systémů u mozkových metastáz o mikroRNA profilování s cílem individualizace pooperační péče

Investor: Ministerstvo zdravotnictví ČR, Rozšíření současných prognostických skórovacích systémů u mozkových metastáz o mikroRNA profilování s cílem individualizace pooperační péče

Citovat

RUČKOVÁ, Michaela; Dagmar AL TUKMACHI; Marek VEČEŘA; Tereza DEISSOVÁ; Robin JUGAS; Markéta HERMANOVÁ; Michal HENDRYCH; Leoš KŘEN; Pavel FADRUS; Václav VYBÍHAL; Hana VALEKOVÁ; Radim JANČÁLEK; Tomáš KAZDA; Ondřej SLABÝ; Martin SMRČKA a Jiří ŠÁNA. Circulating MicroRNAs do not provide a diagnostic benefit over tissue biopsy in patients with brain metastases. Nature Scientific Reports. Berlin: NATURE RESEARCH, 2025, roč. 16, č. 1, s. 1-16. ISSN 2045-2322. Dostupné z: https://doi.org/10.1038/s41598-025-31344-z.

@article{2556697,
   author = {Ručková, Michaela and Al Tukmachi, Dagmar and Večeřa, Marek and Deissová, Tereza and Jugas, Robin and Hermanová, Markéta and Hendrych, Michal and Křen, Leoš and Fadrus, Pavel and Vybíhal, Václav and Valeková, Hana and Jančálek, Radim and Kazda, Tomáš and Slabý, Ondřej and Smrčka, Martin and Šána, Jiří},
   article_location = {Berlin},
   article_number = {1},
   doi = {https://doi.org/10.1038/s41598-025-31344-z},
   keywords = {Brain metastases; MicroRNAs; Liquid biopsies; Diagnostics; Small RNA sequencing},
   language = {eng},
   issn = {2045-2322},
   journal = {Nature Scientific Reports},
   title = {Circulating MicroRNAs do not provide a diagnostic benefit over tissue biopsy in patients with brain metastases},
   url = {https://www.nature.com/articles/s41598-025-31344-z},
   volume = {16},
   year = {2025}
}

TY  - JOUR
ID  - 2556697
AU  - Ručková, Michaela - Al Tukmachi, Dagmar - Večeřa, Marek - Deissová, Tereza - Jugas, Robin - Hermanová, Markéta - Hendrych, Michal - Křen, Leoš - Fadrus, Pavel - Vybíhal, Václav - Valeková, Hana - Jančálek, Radim - Kazda, Tomáš - Slabý, Ondřej - Smrčka, Martin - Šána, Jiří
PY  - 2025
TI  - Circulating MicroRNAs do not provide a diagnostic benefit over tissue biopsy in patients with brain metastases
JF  - Nature Scientific Reports
VL  - 16
IS  - 1
SP  - 1-16
EP  - 1-16
PB  - NATURE RESEARCH
SN  - 20452322
KW  - Brain metastases
KW  - MicroRNAs
KW  - Liquid biopsies
KW  - Diagnostics
KW  - Small RNA sequencing
UR  - https://www.nature.com/articles/s41598-025-31344-z
N2  - Brain metastases (BMs) are frequent and devastating complications of systemic malignancies, necessitating accurate diagnosis and origin identification for effective treatment strategies. Invasive biopsies are currently required for definitive diagnosis, highlighting the need for less invasive diagnostic approaches and robust biomarkers. Circulating microRNAs (miRNAs) have demonstrated potential as sensitive and specific diagnostic biomarkers in various cancers. Thus, our objective was to identify and compare miRNA profiles in BM tissue, cerebrospinal fluid (CSF), and plasma, with a specific focus on liquid biopsies for diagnostic purposes. Total RNA enriched for miRNAs was isolated from histopathologically confirmed BM tissues (n = 30), corresponding plasma samples (n = 30), and CSF samples (n = 27) obtained from patients with diverse BM types. Small RNA sequencing was employed for miRNA expression profiling. Significantly differentially expressed miRNAs were observed in BM tissues, enabling the differentiation of primary origins, particularly breast, colorectal, renal cell carcinoma, and melanoma metastases. The heterogeneity observed in lung carcinomas also manifested in the corresponding BMs, posing challenges in accurate discrimination from other BMs. While tissue-specific miRNA signatures exhibited the highest precision, our findings suggest low diagnostic potential of circulating miRNAs in CSF and blood plasma for BM patients. Our study represents the first analysis of miRNA expression/levels in a unique set of three biological materials (tissue, blood plasma, CSF) obtained from the same BM patients using small RNA sequencing. The presented results underscore the importance of investigating aberrant miRNA expression/levels in BMs and highlight the low diagnostic utility of circulating miRNAs in patients with BMs.
ER  -

RUČKOVÁ, Michaela; Dagmar AL TUKMACHI; Marek VEČEŘA; Tereza DEISSOVÁ; Robin JUGAS; Markéta HERMANOVÁ; Michal HENDRYCH; Leoš KŘEN; Pavel FADRUS; Václav VYBÍHAL; Hana VALEKOVÁ; Radim JANČÁLEK; Tomáš KAZDA; Ondřej SLABÝ; Martin SMRČKA a Jiří ŠÁNA. Circulating MicroRNAs do not provide a diagnostic benefit over tissue biopsy in patients with brain metastases. \textit{Nature Scientific Reports}. Berlin: NATURE RESEARCH, 2025, roč.~16, č.~1, s.~1-16. ISSN~2045-2322. Dostupné z: https://doi.org/10.1038/s41598-025-31344-z.

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