Molecular markers for gyrodactylids (Gyrodactylidae: Monogenea)
from five fish families (Teleostei)

J 2001

Molecular markers for gyrodactylids (Gyrodactylidae: Monogenea) from five fish families (Teleostei)

MATĚJUSOVÁ, Iveta, Milan GELNAR, Alastair J. A. MCBEATH, Catherine M. COLLINS, Carey O. CUNNINGHAM et. al.

Základní údaje

Originální název

Molecular markers for gyrodactylids (Gyrodactylidae: Monogenea) from five fish families (Teleostei)

Autoři

MATĚJUSOVÁ, Iveta, Milan GELNAR, Alastair J. A. MCBEATH, Catherine M. COLLINS a Carey O. CUNNINGHAM

Vydání

International Journal for Parasitology, Australie, Elsevier Science, 2001, 0020-7519

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Austrálie

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.814

Kód RIV

RIV/00216224:14310/01:00004169

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Gyrodactylus; rRNA; Polymerase chain reaction; Internal transcribed spacers; species-level identification

Štítky

gyrodactylus, Internal transcribed spacers, polymerase chain reaction, rRNA, species-level identification

Změněno: 1. 5. 2001 14:51, Mgr. Iveta Matějusová, Ph.D.

Anotace

V originále

Thirty-one gyrodactylid species from five families of freshwater fish were examined and variable region V4 of the 18S small subunit ribosomal RNA gene and ribosomal RNA internal transcribed spacers ITS1 and ITS2 were sequenced. Both the V4 region and spacers ITS1 and ITS2 proved useful for gyrodactylid diagnosis. Sequences of these fragments exhibited interspecific variations and allowed clear determination at the species level. In some cases, the length of the ITS1 PCR fragment provided useful genetic markers. Species that yielded a short ITS1 fragment also showed distinct groupings in ITS2 and V4 sequences that were markedly different to sequences from species that contain a long ITS1. Repetitive sequences located in the ITS1 of Gyrodactylus gobii and Gyrodactylus vimbi accounted for some of the variations in length of PCR products. There was no evidence for intraspecific variation within these regions and short tandem repeats were not found in the other species studied. The number of polymorphic and intraspecific variations in nucleic acid sequences was low, therefore these variations did not affect species determination of gyrodactylids. Minor differences in the sequenced between Western and Eastern European populations were detected for Gyrodactylus salaris/thymalli, Gyrodactylus teuchis and Gyrodactylus truttae, but these do not affect species diagnosis based on ribosomal DNA sequences. These results confirm the utility of both variable region V4 and the ITS as molecular markers for Gyrodactylus species.

Návaznosti

GA524/00/0844, projekt VaV

Název: Neurobiologie, stavba tegumentu a segregace mikrohabitu modelových druhů cizopasníků v průběhu ontogeneze

Investor: Grantová agentura ČR, Neurobiologie, stavba tegumentu a segregace mikrohabitu modelových druhů cizopasníků v průběhu ontogeneze

GA524/98/0940, projekt VaV

Název: Diverzita parazitů jako indikátor enviromentálního stresu

Investor: Grantová agentura ČR, Diverzita parazitů jako indikátor enviromentálního stresu

MSM 143100010, záměr

Název: Časoprostorová dynamika biodiverzity v ekosystémech střední Evropy.

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Časoprostorová dynamika biodiverzity v ekosystémech střední Evropy

Citovat

MATĚJUSOVÁ, Iveta, Milan GELNAR, Alastair J. A. MCBEATH, Catherine M. COLLINS a Carey O. CUNNINGHAM. Molecular markers for gyrodactylids (Gyrodactylidae: Monogenea) from five fish families (Teleostei). International Journal for Parasitology. Australie: Elsevier Science, 2001, roč. 31, č. 4, s. 738-745. ISSN 0020-7519.

@article{361412,
   author = {Matějusová, Iveta and Gelnar, Milan and McBeath, Alastair J. A. and Collins, Catherine M. and Cunningham, Carey O.},
   article_location = {Australie},
   article_number = {4},
   keywords = {Gyrodactylus; rRNA; Polymerase chain reaction; Internal transcribed spacers; species-level identification},
   language = {eng},
   issn = {0020-7519},
   journal = {International Journal for Parasitology},
   title = {Molecular markers for gyrodactylids (Gyrodactylidae: Monogenea) from five fish families (Teleostei)},
   volume = {31},
   year = {2001}
}

TY  - JOUR
ID  - 361412
AU  - Matějusová, Iveta - Gelnar, Milan - McBeath, Alastair J. A. - Collins, Catherine M. - Cunningham, Carey O.
PY  - 2001
TI  - Molecular markers for gyrodactylids (Gyrodactylidae: Monogenea) from five fish families (Teleostei)
JF  - International Journal for Parasitology
VL  - 31
IS  - 4
SP  - 738
EP  - 738
PB  - Elsevier Science
SN  - 00207519
KW  - Gyrodactylus
KW  - rRNA
KW  - Polymerase chain reaction
KW  - Internal transcribed spacers
KW  - species-level identification
N2  - Thirty-one gyrodactylid species from five families of freshwater fish were examined and variable region V4 of the 18S small subunit ribosomal RNA gene and ribosomal RNA internal transcribed spacers ITS1 and ITS2 were sequenced. Both the V4 region and spacers ITS1 and ITS2 proved useful for gyrodactylid diagnosis. Sequences of these fragments exhibited interspecific variations and allowed clear determination at the species level. In some cases, the length of the ITS1 PCR fragment provided useful genetic markers. Species that yielded a short ITS1 fragment also showed distinct groupings in ITS2 and V4 sequences that were markedly different to sequences from species that contain a long ITS1. Repetitive sequences located in the ITS1 of Gyrodactylus gobii and Gyrodactylus vimbi accounted for some of the variations in length of PCR products. There was no evidence for intraspecific variation within these regions and short tandem repeats were not found in the other species studied. The number of polymorphic and intraspecific variations in nucleic acid sequences was low, therefore these variations did not affect species determination of gyrodactylids. Minor differences in the sequenced between Western and Eastern European populations were detected for Gyrodactylus salaris/thymalli, Gyrodactylus teuchis and Gyrodactylus truttae, but these do not affect species diagnosis based on ribosomal DNA sequences. These results confirm the utility of both variable region V4 and the ITS as molecular markers for Gyrodactylus species.
ER  -

MATĚJUSOVÁ, Iveta, Milan GELNAR, Alastair J. A. MCBEATH, Catherine M. COLLINS a Carey O. CUNNINGHAM. Molecular markers for gyrodactylids (Gyrodactylidae: Monogenea) from five fish families (Teleostei). \textit{International Journal for Parasitology}. Australie: Elsevier Science, 2001, roč.~31, č.~4, s.~738-745. ISSN~0020-7519.

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