Glycosylated major urinary protein of the house mouse: characterization of its N-linked oligosaccharides

MECHREF, Yehiya, Lukáš ŽÍDEK, Weidong MA a Milos NOVOTNY. Glycosylated major urinary protein of the house mouse: characterization of its N-linked oligosaccharides. Glycobiology. Oxford: Oxford University Press, roč. 10, č. 3, s. 231-235. ISSN 0959-6658. 2000.

Základní údaje

• Originální název: Glycosylated major urinary protein of the house mouse: characterization of its N-linked oligosaccharides
• Autoři: MECHREF, Yehiya, Lukáš ŽÍDEK, Weidong MA a Milos NOVOTNY.
• Vydání: Glycobiology, Oxford, Oxford University Press, 2000, 0959-6658.

Další údaje

• Typ výsledku: Článek v odborném periodiku
• Utajení: není předmětem státního či obchodního tajemství
• Impakt faktor: Impact factor: 3.419
• Organizační jednotka: Přírodovědecká fakulta
• Klíčová slova anglicky: MASS-SPECTROMETRY; VOMERONASAL ORGAN; MUS-MUSCULUS; GENES; MATRIX; FAMILY; MICE
• Štítky: Family, GENES, MASS-SPECTROMETRY, matrix, mice, MUS-MUSCULUS, VOMERONASAL ORGAN

Anotace

A minor component of the major urinary protein complex of the house mouse was chromatographically isolated and ascertained to be a previously suspected glycoprotein. Using highly sensitive mass-spectrometric techniques for sequencing and linkage analysis, the N-linked oligosaccharides of this glycoprotein were characterized. They were determined to be of the complex type with a wide heterogeneity. The heterogeneity was due to both the degree of sialylation and the presence of galactose residues in either beta(1-3) or beta(1-4) linkages. The biantennary structures were the most pronounced glycans, while tri- and tetraantennary entities were minor.