| ZEHNULOVÁ, Jana, Jana KAŠPÁRKOVÁ, Nicholas FARRELL and Viktor BRABEC. Conformation, recognition by high mobility group domain proteins, and nucleotide excision repair of DNA intrastrand cross-links of novel antitumor trinuclear platinum complex BBR3464. Journal of Biological Chemistry. Bethesda, USA: Amer. Soc. Biochem. Mol., vol. 276, No 25, p. 22191-22199. ISSN 0021-9258. 2001. |
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@article{365796,
author = {Zehnulová, Jana and Kašpárková, Jana and Farrell, Nicholas and Brabec, Viktor},
article_location = {Bethesda, USA},
article_number = {25},
keywords = {DNA; anticancer drug; platinum; conformation; recognition},
language = {eng},
issn = {0021-9258},
journal = {Journal of Biological Chemistry},
title = {Conformation, recognition by high mobility group domain proteins, and nucleotide excision repair of DNA intrastrand cross-links of novel antitumor trinuclear platinum complex BBR3464},
volume = {276},
year = {2001}
}
TY - JOUR
ID - 365796
AU - Zehnulová, Jana - Kašpárková, Jana - Farrell, Nicholas - Brabec, Viktor
PY - 2001
TI - Conformation, recognition by high mobility group domain proteins, and nucleotide excision repair of DNA intrastrand cross-links of novel antitumor trinuclear platinum complex BBR3464
JF - Journal of Biological Chemistry
VL - 276
IS - 25
SP - 22191
EP - 22191
PB - Amer. Soc. Biochem. Mol.
SN - 00219258
KW - DNA
KW - anticancer drug
KW - platinum
KW - conformation
KW - recognition
N2 - The new antitumor trinuclear platinum compound [{trans-PtCl(NH3)2}2m- trans-Pt(NH3)2{H2N(CH2)6NH2}2]4+ (designated as BBR3464) is currently in Phase II clinical trials. DNA is generally considered the major pharmacological target of platinum drugs. The bifunctional DNA binding of BBR3464 is characterized by the rapid formation of long-range intra- and interstrand cross-links. We examined how the structures of the various types of the intrastrand cross-links of BBR3464 affect conformational properties of DNA, how these adducts are recognized by HMG1 protein and removed from DNA during in vitro nucleotide excision repair reactions. The results have revealed that intrastrand cross-links of BBR3464 create a local conformational distortion, but none of these cross-links results in a stable curvature. In addition, we have observed no recognition of these cross-links by HMG1 proteins, but we have observed effective removal of these adducts from DNA by nucleotide excision repair.
ER -
ZEHNULOVÁ, Jana, Jana KAŠPÁRKOVÁ, Nicholas FARRELL and Viktor BRABEC. Conformation, recognition by high mobility group domain proteins, and nucleotide excision repair of DNA intrastrand cross-links of novel antitumor trinuclear platinum complex BBR3464. \textit{Journal of Biological Chemistry}. Bethesda, USA: Amer. Soc. Biochem. Mol., vol.~276, No~25, p.~22191-22199. ISSN~0021-9258. 2001.
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