SLANINOVÁ, Iva, Eva TÁBORSKÁ, Hana BOCHOŘÁKOVÁ and Jiří SLANINA. Interaction of benzo[c]phenanthridine and protoberberine alkaloids with animal and yeast cells. Cell Biology and Toxicology. Dordrecht, Netherlands: Kluwer Academic Publishers, 2001, vol. 17, No 1, p. 51-63. ISSN 0742-2091.
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Basic information
Original name Interaction of benzo[c]phenanthridine and protoberberine alkaloids with animal and yeast cells
Authors SLANINOVÁ, Iva (203 Czech Republic, guarantor), Eva TÁBORSKÁ (203 Czech Republic), Hana BOCHOŘÁKOVÁ (203 Czech Republic) and Jiří SLANINA (203 Czech Republic).
Edition Cell Biology and Toxicology, Dordrecht, Netherlands, Kluwer Academic Publishers, 2001, 0742-2091.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher Netherlands
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 1.177
RIV identification code RIV/00216224:14110/01:00004307
Organization unit Faculty of Medicine
UT WoS 000169125300006
Keywords in English benzo[c]phenanthridine; protoberberine alkaloids; cytotoxicity; yeast; cytoskeleton; microtubules
Tags benzo[c]phenanthridine, Cytoskeleton, cytotoxicity, Microtubules, protoberberine alkaloids, Yeast
Changed by Changed by: prof. MUDr. Iva Slaninová, Ph.D., učo 2105. Changed: 22/6/2009 12:17.
We compared the effects of four quaternary benzo[c]phenanthridine alkaloids, i.e., chelerythrine, chelilutine, sanguinarine and sanguilutine, and two quaternary protoberberine alkaloids, berberine and coptisine, on the human cell line HeLa (cervix carcinoma cells) and the yeasts Saccharomyces cerevisiae and Schizosaccharomyces japonicus var. versatilis. The ability of alkaloids to display primary fluorescence, allowed us to record their dynamics and localization in cells. Cytotoxicity, anti-microtubular and anti-actin effects in living cells were studied. In the yeasts, neither microtubules nor cell growth were seriously affected even at the alkaloid concentration of 100 mg/mL. The HeLa cells, however, responded to the toxic effect of alkaloids at concentrations ranging from 1-50 mg/mL. IC50 values for individual alkaloids were: sanguinarine IC50 = 0.8 mg/mL, sanguilutine IC50 = 8.3 mg/mL, chelerythrine IC50 = 6.2 mg/mL, chelilutine IC50 = 5.2 mg/mL, coptisine IC50 = 2.6 mg/mL and berberine IC50 =10.0 mg/mL. In living cells, sanguinarine produced a decrease in microtubule numbers, particularly at the cell periphery, at a concentration of 0.1 mg/mL The other alkaloids show the similar effect but at higher concentrations (5 to 10 mg/mL). The strongest effects of sanguinarine were explained as a consequence of its easy penetration through the cell membrane due to non-polar pseudobase formation and to a high degree of molecular planarity.
GA204/99/D025, research and development projectName: Úloha cytoskeletu a buněčné stěny v polarizaci buňky
Investor: Czech Science Foundation, The role of cytoskeleton and cell wall in the cell polarization
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