Activation or detoxification of mutagenic and carcinogenic
compounds in transgenic Drosophila expressing human glutathione
S-transferase.

J 2000

Activation or detoxification of mutagenic and carcinogenic compounds in transgenic Drosophila expressing human glutathione S-transferase.

CHROUST, Karel; Trevor JOWETT; Mustafa FARID-WAJIDI; Juan HUANG; Martina RYŠKOVÁ et. al.

Základní údaje

Originální název

Activation or detoxification of mutagenic and carcinogenic compounds in transgenic Drosophila expressing human glutathione S-transferase.

Autoři

CHROUST, Karel; Trevor JOWETT; Mustafa FARID-WAJIDI; Juan HUANG; Martina RYŠKOVÁ; Ronald WOLF a Ivan HOLOUBEK

Vydání

Mutation Research, Amsterdam, Elsevier, 2000, 0165-1218

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/00:00004528

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Transgenic Drosophila melanogaster; wing spot test; female sterility; aneuploidy; glutathione S-transferase; 1;2:5;6-dibenzanthracene; 1;2-dichloroethane

Štítky

1, 2-dichloroethane, 2:5, 6-dibenzanthracene, aneuploidy, female sterility, glutathione S-transferase, transgenic Drosophila melanogaster, wing spot test

Změněno: 15. 1. 2002 08:49, prof. RNDr. Luděk Bláha, Ph.D.

Anotace

V originále

Sensitivity of transgenic Drosophila melanogaster with expression of a human gene encoding the glutathione S-transferase alpha subunit (GSTA1-1) to 1,2:5,6-dibenzanthracene (DBA) and 1,2-dichloroethane (DCE) was investigated in the somatic mutation and recombination test (SMART). We performed the same assay in control transgenic flies expressing the bacterial lacZ gene. Three types of transgenic Drosophila strains carrying GSTA1-1 were used: two transgenic strains homozygous for the second chromosome with a single-copy transgene insertion and one strain with two transgene insertions. Larvae carrying the lacZ gene were significantly more sensitive to genotoxic effects of DBA than those carrying three copies of the GSTA1-1 gene. The larvae with lacZ expression showed significantly lower sensitivity to DCE compared with those expressing GSTA1-1. Finally, a pretreatment with buthionine-sulphoximine (BSO) in experiment with DCE significantly decreased the frequency of mutation events in larvae with three GSTA1-1 copies in comparison with others.

Návaznosti

MSM 141100003, záměr

Název: Zevní prostředí - karcinogeneze - onkologie

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Zevní prostředí - karcinogeneze - onkologie

MSM 143100008, záměr

Název: Genomy a jejich funkce

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Genomy a jejich funkce

Citovat

CHROUST, Karel; Trevor JOWETT; Mustafa FARID-WAJIDI; Juan HUANG; Martina RYŠKOVÁ; Ronald WOLF a Ivan HOLOUBEK. Activation or detoxification of mutagenic and carcinogenic compounds in transgenic Drosophila expressing human glutathione S-transferase. Mutation Research. Amsterdam: Elsevier, 2000, roč. 2001, 498(1-2), s. 169-179. ISSN 0165-1218.

@article{371654,
   author = {Chroust, Karel and Jowett, Trevor and FaridandWajidi, Mustafa and Huang, Juan and Ryšková, Martina and Wolf, Ronald and Holoubek, Ivan},
   article_location = {Amsterdam},
   article_number = {498(1-2)},
   keywords = {Transgenic Drosophila melanogaster; wing spot test; female sterility; aneuploidy; glutathione S-transferase; 1;2:5;6-dibenzanthracene; 1;2-dichloroethane},
   language = {eng},
   issn = {0165-1218},
   journal = {Mutation Research},
   title = {Activation or detoxification of mutagenic and carcinogenic compounds in transgenic Drosophila expressing human glutathione S-transferase.},
   volume = {2001},
   year = {2000}
}

TY  - JOUR
ID  - 371654
AU  - Chroust, Karel - Jowett, Trevor - Farid-Wajidi, Mustafa - Huang, Juan - Ryšková, Martina - Wolf, Ronald - Holoubek, Ivan
PY  - 2000
TI  - Activation or detoxification of mutagenic and carcinogenic compounds in transgenic Drosophila expressing human glutathione S-transferase.
JF  - Mutation Research
VL  - 2001
IS  - 498(1-2)
SP  - 169
EP  - 169
PB  - Elsevier
SN  - 01651218
KW  - Transgenic Drosophila melanogaster
KW  - wing spot test
KW  - female sterility
KW  - aneuploidy
KW  - glutathione S-transferase
KW  - 1;2:5;6-dibenzanthracene
KW  - 1;2-dichloroethane
N2  - Sensitivity of transgenic Drosophila melanogaster with expression of a human gene encoding the glutathione S-transferase alpha subunit (GSTA1-1) to 1,2:5,6-dibenzanthracene (DBA) and 1,2-dichloroethane (DCE) was investigated in the somatic mutation and recombination test (SMART). We performed the same assay in control transgenic flies expressing the bacterial lacZ gene. Three types of transgenic Drosophila strains carrying GSTA1-1 were used: two transgenic strains homozygous for the second chromosome with a single-copy transgene insertion and one strain with two transgene insertions. Larvae carrying the lacZ gene were significantly more sensitive to genotoxic effects of DBA than those carrying three copies of the GSTA1-1 gene. The larvae with lacZ expression showed significantly lower sensitivity to DCE compared with those expressing GSTA1-1. Finally, a pretreatment with buthionine-sulphoximine (BSO) in experiment with DCE significantly decreased the frequency of mutation events in larvae with three GSTA1-1 copies in comparison with others.
ER  -

CHROUST, Karel; Trevor JOWETT; Mustafa FARID-WAJIDI; Juan HUANG; Martina RYŠKOVÁ; Ronald WOLF a Ivan HOLOUBEK. Activation or detoxification of mutagenic and carcinogenic compounds in transgenic Drosophila expressing human glutathione S-transferase. \textit{Mutation Research}. Amsterdam: Elsevier, 2000, roč.~2001, 498(1-2), s.~169-179. ISSN~0165-1218.

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