| RITTICH, Bohuslav, Alena ŠPANOVÁ, Yuriy OHLASHENNYY, Ivo RUDOLF, Daniel HORÁK, Jiří LENFELD a Milan J. BENEŠ. Influence of metal ions on activity and stability of DNase I immobilized on hydrophilic particles. In The 4th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists. 2001. vyd. Zagreb, Croatia: Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia, 2001, s. 63-64. |
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@inproceedings{378552,
author = {Rittich, Bohuslav and Španová, Alena and Ohlashennyy, Yuriy and Rudolf, Ivo and Horák, Daniel and Lenfeld, Jiří and Beneš, Milan J.},
address = {Zagreb, Croatia},
booktitle = {The 4th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists},
edition = {2001},
keywords = {immobilization; DNase I; activity; stability; applications},
language = {eng},
location = {Zagreb, Croatia},
pages = {63-64},
publisher = {Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia},
title = {Influence of metal ions on activity and stability of DNase I immobilized on hydrophilic particles},
year = {2001}
}
TY - JOUR
ID - 378552
AU - Rittich, Bohuslav - Španová, Alena - Ohlashennyy, Yuriy - Rudolf, Ivo - Horák, Daniel - Lenfeld, Jiří - Beneš, Milan J.
PY - 2001
TI - Influence of metal ions on activity and stability of DNase I immobilized on hydrophilic particles
PB - Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia
CY - Zagreb, Croatia
KW - immobilization
KW - DNase I
KW - activity
KW - stability
KW - applications
N2 - The aim of work has been concentrated on degradation of high-weight and plasmid DNAs using immobilized DNase I. The activity of free and immobilized DNase I was estimated from UV spectrophotometric measurements and from agarose gel electrophoresis. The immobilized enzyme was full actived using Co2+ ions as an activator, a decrease of the activity was observed using other ions. Practical applications of DNase I immobilized on poly(HEMA-co-EDMA) microspheres in cleavage of plasmid DNAs are discussed.
ER -
RITTICH, Bohuslav, Alena ŠPANOVÁ, Yuriy OHLASHENNYY, Ivo RUDOLF, Daniel HORÁK, Jiří LENFELD a Milan J. BENEŠ. Influence of metal ions on activity and stability of DNase I immobilized on hydrophilic particles. In \textit{The 4th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists}. 2001. vyd. Zagreb, Croatia: Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia, 2001, s.~63-64.
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