v-Jun and v-Fos proteins induce dedifferentiation of
v-myb-transformed monoblasts

D 2001

v-Jun and v-Fos proteins induce dedifferentiation of v-myb-transformed monoblasts

ŠEVČÍKOVÁ, Sabina, Eva ZAHRADNÍČKOVÁ, Karel SOUČEK a Jan ŠMARDA

Základní údaje

Originální název

v-Jun and v-Fos proteins induce dedifferentiation of v-myb-transformed monoblasts

Autoři

ŠEVČÍKOVÁ, Sabina (203 Česká republika), Eva ZAHRADNÍČKOVÁ (203 Česká republika), Karel SOUČEK (203 Česká republika) a Jan ŠMARDA (203 Česká republika, garant)

Vydání

Frederick, Maryland, USA, The Seventeenth Annual Meeting on Oncogene, s. 171-171, 2001

Nakladatel

Foundation for Advanced Cancer Studies, Inc.

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

30200 3.2 Clinical medicine

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/01:00004846

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Myb; Fos; Jun; differentiation

Štítky

differentiation, Fos, Jun, Myb

Změněno: 17. 2. 2009 05:35, prof. RNDr. Jan Šmarda, CSc.

Anotace

V originále

The fos/jun proto-oncogenes encode for proteins which are components of the AP-1 transcription complex that includes several Fos related and Jun related proteins. The AP-1 was shown to play multiple roles in functional development of hematopoietic precursor cells into mature blood cells. Proto-oncogenes of the fos/jun family are considered to be positive regulators of myeloid differentiation. Enforced c-fos and c-jun expression in certain myeloid leukemia cell lines results in increased propensity to differentiate. The line of v-Myb-transformed chicken monoblasts (BM2) can be induced to differentiate phorbol esters. Differentiation-promoting effects of 12-O-tetradecanoyl phorbol 13-acetate (TPA) on BM2 cells is carried though activation of protein kinase C followed by changes of activities of differentiation and proliferation regulators, including AP-1. To gain further insights into the role of fos/jun in leukemogenicity, BM2vJUN and BM2vFOS cell lines, which inducibly express v-jun and v-fos, respectively, were established. We found that either v-fos- or v-jun-expressing BM2 cells exhibit signs of dedifferentiation. They decrease ability to reduce NBT and increase expression of differentiation-specific markers as Mo-1 and MEP-17. This suggests that oncogenic forms of Jun and Fos proteins can further enforce v-Myb transformation.

Návaznosti

GA301/01/0040, projekt VaV

Název: Úloha proteinů Fos a Jun v monocytické diferenciaci stimulované kyselinou retinovou

Investor: Grantová agentura ČR, Úloha proteinů Fos a Jun v monocytické diferenciaci stimulované kyselinou retinovou

MSM 143100008, záměr

Název: Genomy a jejich funkce

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Genomy a jejich funkce

Citovat

ŠEVČÍKOVÁ, Sabina, Eva ZAHRADNÍČKOVÁ, Karel SOUČEK a Jan ŠMARDA. v-Jun and v-Fos proteins induce dedifferentiation of v-myb-transformed monoblasts. In The Seventeenth Annual Meeting on Oncogene. Frederick, Maryland, USA: Foundation for Advanced Cancer Studies, Inc., 2001, s. 171.

@inproceedings{378866,
   author = {Ševčíková, Sabina and Zahradníčková, Eva and Souček, Karel and Šmarda, Jan},
   address = {Frederick, Maryland, USA},
   booktitle = {The Seventeenth Annual Meeting on Oncogene},
   keywords = {Myb; Fos; Jun; differentiation},
   language = {eng},
   location = {Frederick, Maryland, USA},
   pages = {171-171},
   publisher = {Foundation for Advanced Cancer Studies, Inc.},
   title = {v-Jun and v-Fos proteins induce dedifferentiation of v-myb-transformed monoblasts},
   year = {2001}
}

TY  - JOUR
ID  - 378866
AU  - Ševčíková, Sabina - Zahradníčková, Eva - Souček, Karel - Šmarda, Jan
PY  - 2001
TI  - v-Jun and v-Fos proteins induce dedifferentiation of v-myb-transformed monoblasts
PB  - Foundation for Advanced Cancer Studies, Inc.
CY  - Frederick, Maryland, USA
KW  - Myb
KW  - Fos
KW  - Jun
KW  - differentiation
N2  - The fos/jun proto-oncogenes encode for proteins which are components of the AP-1 transcription complex that includes several Fos related and Jun related proteins. The AP-1 was shown to play multiple roles in functional development of hematopoietic precursor cells into mature blood cells. Proto-oncogenes of the fos/jun family are considered to be positive regulators of myeloid differentiation. Enforced c-fos and c-jun expression in certain myeloid leukemia cell lines results in increased propensity to differentiate. The line of v-Myb-transformed chicken monoblasts (BM2) can be induced to differentiate phorbol esters. Differentiation-promoting effects of 12-O-tetradecanoyl phorbol 13-acetate (TPA) on BM2 cells is carried though activation of protein kinase C followed by changes of activities of differentiation and proliferation regulators, including AP-1. To gain further insights into the role of fos/jun in leukemogenicity, BM2vJUN and BM2vFOS cell lines, which inducibly express v-jun and v-fos, respectively, were established. We found that either v-fos- or v-jun-expressing BM2 cells exhibit signs of dedifferentiation. They decrease ability to reduce NBT and increase expression of differentiation-specific markers as Mo-1 and MEP-17. This suggests that oncogenic forms of Jun and Fos proteins can further enforce v-Myb transformation.
ER  -

ŠEVČÍKOVÁ, Sabina, Eva ZAHRADNÍČKOVÁ, Karel SOUČEK a Jan ŠMARDA. v-Jun and v-Fos proteins induce dedifferentiation of v-myb-transformed monoblasts. In \textit{The Seventeenth Annual Meeting on Oncogene}. Frederick, Maryland, USA: Foundation for Advanced Cancer Studies, Inc., 2001, s.~171.

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