A response of yeast cells to heat stress: cell viability and
the stabilitz of cytoskeletal structures

J 2000

A response of yeast cells to heat stress: cell viability and the stabilitz of cytoskeletal structures

HOLUBÁŘOVÁ, Alena; Petr MÜLLER and Augustin SVOBODA

Basic information

Original name

A response of yeast cells to heat stress: cell viability and the stabilitz of cytoskeletal structures

Authors

HOLUBÁŘOVÁ, Alena; Petr MÜLLER and Augustin SVOBODA

Edition

Scripta medica, Brno, MU, 2000, 1211-3395

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

is not subject to a state or trade secret

RIV identification code

RIV/00216224:14110/00:00005108

Organization unit

Faculty of Medicine

Keywords in English

Saccharomyces cerevisiae; heat shock; Hsp104; actin; microtubules

Abstract

In the original language

The cells of Saccharomyces cerevisiae were exposed to the effect of mild and lethal heat stress and their viability and changes in their microtubular and actin structures were studied. An increase in cultivation temperature from 25 C to 46 C (lethal heat shock, LHS) resulted, during 10 min, in a rapid decrease in cell viability. However, a mild increase in incubation temperature from 25 C to 37 C or 41 C (mild heat shock, MHS) induced cell tolerance to LHS and the cells were able to survive at 46 C for up to 30 min. A mild heat shock increased the level of Hsp104 in cells, as demonstrated by western blot analysis and indirect immunofluorescence microscopy. In cell exposed to LHS, immunofluorescence microscopy showed rapid degradation of both cytoplasmic and nuclear microtubules, but the spindle pole bodies (SPB) remained preserved. On the other hand, MHS did not produce microtubule degradation. Microtubules remained stable after the LHS that had been preceded by cell cultivation at 37 C. It is suggested that stress proteins induced by MHS were involved in maintaining microtubule stability. The actin cytoskeleton was very sensitive to heat shock and transfer of cells to both 37 C and 41 C resulted in disintegration of actin filaments and the spreading of actin dots from the bud to the whole cell surface. No stabilisation effect of stress proteins on actin structures was observed. Resistance to LHS was also induced by an osmotic shock in the presence of 1M KCl in culture medium. However, the production of stress proteins in response to osmotic shock was generally slower than to MHS because the cells became resistant to LHS only after being exposed to 1M KCl for 3 h.

Links

GA204/00/0394, research and development project

Name: Buněčná stěna kvasinek jako extracelulární matrix: cytoskelet a stěnové funkce

Investor: Czech Science Foundation, The yeast cell wall as an extracellular matrix: cytoskeleton and cell wall functions

Cite

HOLUBÁŘOVÁ, Alena; Petr MÜLLER and Augustin SVOBODA. A response of yeast cells to heat stress: cell viability and the stabilitz of cytoskeletal structures. Scripta medica. Brno: MU, 2000, vol. 73, No 6, p. 381-391. ISSN 1211-3395.

@article{384149,
   author = {Holubářová, Alena and Müller, Petr and Svoboda, Augustin},
   article_location = {Brno},
   article_number = {6},
   keywords = {Saccharomyces cerevisiae; heat shock; Hsp104; actin; microtubules},
   language = {eng},
   issn = {1211-3395},
   journal = {Scripta medica},
   title = {A response of yeast cells to heat stress: cell viability and the stabilitz of cytoskeletal structures},
   volume = {73},
   year = {2000}
}

TY  - JOUR
ID  - 384149
AU  - Holubářová, Alena - Müller, Petr - Svoboda, Augustin
PY  - 2000
TI  - A response of yeast cells to heat stress: cell viability and the stabilitz of cytoskeletal structures
JF  - Scripta medica
VL  - 73
IS  - 6
SP  - 381
EP  - 381
PB  - MU
SN  - 12113395
KW  - Saccharomyces cerevisiae
KW  - heat shock
KW  - Hsp104
KW  - actin
KW  - microtubules
N2  - The cells of Saccharomyces cerevisiae were exposed to the effect of mild and lethal heat stress and their viability and changes in their microtubular and actin structures were studied. An increase in cultivation temperature from 25 C to 46 C (lethal heat shock, LHS) resulted, during 10 min, in a rapid decrease in cell viability. However, a mild increase in incubation temperature from 25 C to 37 C or 41 C (mild heat shock, MHS) induced cell tolerance to LHS and the cells were able to survive at 46 C for up to 30 min. A mild heat shock increased the level of Hsp104 in cells, as demonstrated by western blot analysis and indirect immunofluorescence microscopy. In cell exposed to LHS, immunofluorescence microscopy showed rapid degradation of both cytoplasmic and nuclear microtubules, but the spindle pole bodies (SPB) remained preserved. On the other hand, MHS did not produce microtubule degradation. Microtubules remained stable after the LHS that had been preceded by cell cultivation at 37 C. It is suggested that stress proteins induced by MHS were involved in maintaining microtubule stability. The actin cytoskeleton was very sensitive to heat shock and transfer of cells to both 37 C and 41 C resulted in disintegration of actin filaments and the spreading of actin dots from the bud to the whole cell surface. No stabilisation effect of stress proteins on actin structures was observed. Resistance to LHS was also induced by an osmotic shock in the presence of 1M KCl in culture medium. However, the production of stress proteins in response to osmotic shock was generally slower than to MHS because the cells became resistant to LHS only after being exposed to 1M KCl for 3 h.
ER  -

HOLUBÁŘOVÁ, Alena; Petr MÜLLER and Augustin SVOBODA. A response of yeast cells to heat stress: cell viability and the stabilitz of cytoskeletal structures. \textit{Scripta medica}. Brno: MU, 2000, vol.~73, No~6, p.~381-391. ISSN~1211-3395.

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