A response of yeast cells to heat stress: cell viability and
the stabilitz of cytoskeletal structures

J 2000

A response of yeast cells to heat stress: cell viability and the stabilitz of cytoskeletal structures

HOLUBÁŘOVÁ, Alena; Petr MÜLLER a Augustin SVOBODA

Základní údaje

Originální název

A response of yeast cells to heat stress: cell viability and the stabilitz of cytoskeletal structures

Autoři

HOLUBÁŘOVÁ, Alena; Petr MÜLLER a Augustin SVOBODA

Vydání

Scripta medica, Brno, MU, 2000, 1211-3395

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14110/00:00005108

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

Saccharomyces cerevisiae; heat shock; Hsp104; actin; microtubules

Štítky

Actin, heat shock, Hsp104, Microtubules, Saccharomyces cerevisiae

Změněno: 19. 12. 2001 15:38, Mgr. Alena Holubářová, Ph.D.

Anotace

V originále

The cells of Saccharomyces cerevisiae were exposed to the effect of mild and lethal heat stress and their viability and changes in their microtubular and actin structures were studied. An increase in cultivation temperature from 25 C to 46 C (lethal heat shock, LHS) resulted, during 10 min, in a rapid decrease in cell viability. However, a mild increase in incubation temperature from 25 C to 37 C or 41 C (mild heat shock, MHS) induced cell tolerance to LHS and the cells were able to survive at 46 C for up to 30 min. A mild heat shock increased the level of Hsp104 in cells, as demonstrated by western blot analysis and indirect immunofluorescence microscopy. In cell exposed to LHS, immunofluorescence microscopy showed rapid degradation of both cytoplasmic and nuclear microtubules, but the spindle pole bodies (SPB) remained preserved. On the other hand, MHS did not produce microtubule degradation. Microtubules remained stable after the LHS that had been preceded by cell cultivation at 37 C. It is suggested that stress proteins induced by MHS were involved in maintaining microtubule stability. The actin cytoskeleton was very sensitive to heat shock and transfer of cells to both 37 C and 41 C resulted in disintegration of actin filaments and the spreading of actin dots from the bud to the whole cell surface. No stabilisation effect of stress proteins on actin structures was observed. Resistance to LHS was also induced by an osmotic shock in the presence of 1M KCl in culture medium. However, the production of stress proteins in response to osmotic shock was generally slower than to MHS because the cells became resistant to LHS only after being exposed to 1M KCl for 3 h.

Návaznosti

GA204/00/0394, projekt VaV

Název: Buněčná stěna kvasinek jako extracelulární matrix: cytoskelet a stěnové funkce

Investor: Grantová agentura ČR, Buněčná stěna kvasinek jako extracelulární matrix: cytoskelet a stěnové funkce

Citovat

HOLUBÁŘOVÁ, Alena; Petr MÜLLER a Augustin SVOBODA. A response of yeast cells to heat stress: cell viability and the stabilitz of cytoskeletal structures. Scripta medica. Brno: MU, 2000, roč. 73, č. 6, s. 381-391. ISSN 1211-3395.

@article{384149,
   author = {Holubářová, Alena and Müller, Petr and Svoboda, Augustin},
   article_location = {Brno},
   article_number = {6},
   keywords = {Saccharomyces cerevisiae; heat shock; Hsp104; actin; microtubules},
   language = {eng},
   issn = {1211-3395},
   journal = {Scripta medica},
   title = {A response of yeast cells to heat stress: cell viability and the stabilitz of cytoskeletal structures},
   volume = {73},
   year = {2000}
}

TY  - JOUR
ID  - 384149
AU  - Holubářová, Alena - Müller, Petr - Svoboda, Augustin
PY  - 2000
TI  - A response of yeast cells to heat stress: cell viability and the stabilitz of cytoskeletal structures
JF  - Scripta medica
VL  - 73
IS  - 6
SP  - 381
EP  - 381
PB  - MU
SN  - 12113395
KW  - Saccharomyces cerevisiae
KW  - heat shock
KW  - Hsp104
KW  - actin
KW  - microtubules
N2  - The cells of Saccharomyces cerevisiae were exposed to the effect of mild and lethal heat stress and their viability and changes in their microtubular and actin structures were studied. An increase in cultivation temperature from 25 C to 46 C (lethal heat shock, LHS) resulted, during 10 min, in a rapid decrease in cell viability. However, a mild increase in incubation temperature from 25 C to 37 C or 41 C (mild heat shock, MHS) induced cell tolerance to LHS and the cells were able to survive at 46 C for up to 30 min. A mild heat shock increased the level of Hsp104 in cells, as demonstrated by western blot analysis and indirect immunofluorescence microscopy. In cell exposed to LHS, immunofluorescence microscopy showed rapid degradation of both cytoplasmic and nuclear microtubules, but the spindle pole bodies (SPB) remained preserved. On the other hand, MHS did not produce microtubule degradation. Microtubules remained stable after the LHS that had been preceded by cell cultivation at 37 C. It is suggested that stress proteins induced by MHS were involved in maintaining microtubule stability. The actin cytoskeleton was very sensitive to heat shock and transfer of cells to both 37 C and 41 C resulted in disintegration of actin filaments and the spreading of actin dots from the bud to the whole cell surface. No stabilisation effect of stress proteins on actin structures was observed. Resistance to LHS was also induced by an osmotic shock in the presence of 1M KCl in culture medium. However, the production of stress proteins in response to osmotic shock was generally slower than to MHS because the cells became resistant to LHS only after being exposed to 1M KCl for 3 h.
ER  -

HOLUBÁŘOVÁ, Alena; Petr MÜLLER a Augustin SVOBODA. A response of yeast cells to heat stress: cell viability and the stabilitz of cytoskeletal structures. \textit{Scripta medica}. Brno: MU, 2000, roč.~73, č.~6, s.~381-391. ISSN~1211-3395.

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