J 2001

Cytoskeleton in regenerating protoplasts and restoration of cell polarity in the yeast Saccharomyces cerevisiae

SVOBODA, Augustin, Iva SLANINOVÁ and Alena HOLUBÁŘOVÁ

Basic information

Original name

Cytoskeleton in regenerating protoplasts and restoration of cell polarity in the yeast Saccharomyces cerevisiae

Authors

SVOBODA, Augustin, Iva SLANINOVÁ and Alena HOLUBÁŘOVÁ

Edition

Acta Biologica Hungarica, Budapest, Akademiai Kiado, 2001, 0236-5383-01

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Hungary

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14110/01:00005112

Organization unit

Faculty of Medicine

UT WoS

000169642400017

Keywords in English

yeast; protoplasts; cell wall; actin; microtubules; cell polarity

Tags

Změněno: 19/12/2001 15:47, prof. MUDr. Augustin Svoboda, CSc., dr. h.c.

Abstract

V originále

The actin cables and microtubules were disrupted during protoplasting in Saccharomyces cerevisiae cells. In the process of protoplast regeneration, the cytoplasmic microtubules were the first to be restored; actin patches remained regularly distributed under the surface of growing protoplast. After the cell wall had been regenerated in a gelatine medium, actin patches aggregated into clusters, which marked the site of bud development. An incomplete cell wall was the apparent cause for uncoupling between karyokinesis and cytokinesis The presence of several nuclei in the cell gave rise to several buds emerging simultaneously and was probably related to their random positions. In haploids, however, the axial type of budding was obvious also in the reverting protoplasts. Our observations suggest that actin is a structure involved in the restoration of polar growth during protoplast regeneration and that the cell wall plays an important role in this process: in its absence, actin fails to polarise.

Links

GA204/00/0394, research and development project
Name: Buněčná stěna kvasinek jako extracelulární matrix: cytoskelet a stěnové funkce
Investor: Czech Science Foundation, The yeast cell wall as an extracellular matrix: cytoskeleton and cell wall functions
GA204/99/D025, research and development project
Name: Úloha cytoskeletu a buněčné stěny v polarizaci buňky
Investor: Czech Science Foundation, The role of cytoskeleton and cell wall in the cell polarization