Immunomicroscopical studies of the neuromuscular system of the
monogenean, Eudiplozoon nipponicum.

D 2001

Immunomicroscopical studies of the neuromuscular system of the monogenean, Eudiplozoon nipponicum.

ZURAWSKI, Tomáš, A. MOUSLEY, G. MAIR, A. BRENNAN, A. MAULE et. al.

Základní údaje

Originální název

Immunomicroscopical studies of the neuromuscular system of the monogenean, Eudiplozoon nipponicum.

Autoři

ZURAWSKI, Tomáš, A. MOUSLEY, G. MAIR, A. BRENNAN, A. MAULE, Milan GELNAR a D. HALTON

Vydání

2001. vyd. Keele, U.K. Abstract of Spring Meeting of the British Society for Parasitology, British Society for Parasitology. s. 19-19, 2001

Nakladatel

University of Keele, Keele, U.K.

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/01:00005367

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

monogenea; neuromascular system; confocal microscopy; electron microscopy

Štítky

confocal microscopy, electron microscopy, Monogenea, neuromascular system

Změněno: 18. 1. 2002 15:45, Mgr. Tomáš Zurawski, Ph.D.

Anotace

V originále

We have examined microscopically the neuromusculature of Eudiplozoon nipponicum to explore neuromuscular involvement in fusion. Cholinergic, aminergic and peptidergic neuronal elements were demonstrated by enzyme- and immuno-cytochemistry in conjunction with confocal microscopy. FITC-labelled phalloidin was a probe for F-actin. Electron immunogold-labeling revealed peptide immunoreactivity subcellularly. The nervous system was strongly reactive for co-localised cholinergic and peptidergic pathways and for separate pathways of aminergic elements. There was strong innervation to the pharnyx, attachment sucker and clamps of the diporpa. Neuronal pathways of worm pairs are separate, with no obvious fusion. Muscle systems are dominated by extensive somatic fibres, buccal suckers, haptoral clamps and, in early stages, a transient ventral sucker. Diagonal fibres largely comprise the diporpa body wall and longitudinal muscle bundles run from forebody to haptor. EM confirmed neuropeptide-immunolocalisation in dense-cored vesicles in axons of both CNS and PNS. The structural complexity of the neuromusculature reflects its importance in host attachment, and in the processes of body contact, twisting/fusion in larval pairing.

Návaznosti

GA524/00/0844, projekt VaV

Název: Neurobiologie, stavba tegumentu a segregace mikrohabitu modelových druhů cizopasníků v průběhu ontogeneze

Investor: Grantová agentura ČR, Neurobiologie, stavba tegumentu a segregace mikrohabitu modelových druhů cizopasníků v průběhu ontogeneze

MSM 143100010, záměr

Název: Časoprostorová dynamika biodiverzity v ekosystémech střední Evropy.

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Časoprostorová dynamika biodiverzity v ekosystémech střední Evropy

Citovat

ZURAWSKI, Tomáš, A. MOUSLEY, G. MAIR, A. BRENNAN, A. MAULE, Milan GELNAR a D. HALTON. Immunomicroscopical studies of the neuromuscular system of the monogenean, Eudiplozoon nipponicum. In Abstract of Spring Meeting of the British Society for Parasitology, British Society for Parasitology. 2001. vyd. Keele, U.K.: University of Keele, Keele, U.K., 2001, s. 19.

@inproceedings{390060,
   author = {Zurawski, Tomáš and Mousley, A. and Mair, G. and Brennan, A. and Maule, A. and Gelnar, Milan and Halton, D.},
   address = {Keele, U.K.},
   booktitle = {Abstract of Spring Meeting of the British Society for Parasitology, British Society for Parasitology.},
   edition = {2001},
   keywords = {monogenea; neuromascular system; confocal microscopy; electron microscopy},
   language = {eng},
   location = {Keele, U.K.},
   pages = {19-19},
   publisher = {University of Keele, Keele, U.K.},
   title = {Immunomicroscopical studies of the neuromuscular system of the monogenean, Eudiplozoon nipponicum.},
   year = {2001}
}

TY  - JOUR
ID  - 390060
AU  - Zurawski, Tomáš - Mousley, A. - Mair, G. - Brennan, A. - Maule, A. - Gelnar, Milan - Halton, D.
PY  - 2001
TI  - Immunomicroscopical studies of the neuromuscular system of the monogenean, Eudiplozoon nipponicum.
PB  - University of Keele, Keele, U.K.
CY  - Keele, U.K.
KW  - monogenea
KW  - neuromascular system
KW  - confocal microscopy
KW  - electron microscopy
N2  - We have examined microscopically the neuromusculature of Eudiplozoon nipponicum to explore neuromuscular involvement in fusion. Cholinergic, aminergic and peptidergic neuronal elements were demonstrated by enzyme- and immuno-cytochemistry in conjunction with confocal microscopy. FITC-labelled phalloidin was a probe for F-actin. Electron immunogold-labeling revealed peptide immunoreactivity subcellularly. The nervous system was strongly reactive for co-localised cholinergic and peptidergic pathways and for separate pathways of aminergic elements. There was strong innervation to the pharnyx, attachment sucker and clamps of the diporpa. Neuronal pathways of worm pairs are separate, with no obvious fusion. Muscle systems are dominated by extensive somatic fibres, buccal suckers, haptoral clamps and, in early stages, a transient ventral sucker. Diagonal fibres largely comprise the diporpa body wall and longitudinal muscle bundles run from forebody to haptor. EM confirmed neuropeptide-immunolocalisation in dense-cored vesicles in axons of both CNS and PNS. The structural complexity of the neuromusculature reflects its importance in host attachment, and in the processes of body contact, twisting/fusion in larval pairing.
ER  -

ZURAWSKI, Tomáš, A. MOUSLEY, G. MAIR, A. BRENNAN, A. MAULE, Milan GELNAR a D. HALTON. Immunomicroscopical studies of the neuromuscular system of the monogenean, Eudiplozoon nipponicum. In \textit{Abstract of Spring Meeting of the British Society for Parasitology, British Society for Parasitology.}. 2001. vyd. Keele, U.K.: University of Keele, Keele, U.K., 2001, s.~19.

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