Elimination Voltammetry of Adenine and Cytosine  Mixtures

J 2001

Elimination Voltammetry of Adenine and Cytosine Mixtures

TRNKOVÁ, Libuše; Jiří FRIML a Oldřich DRAČKA

Základní údaje

Originální název

Elimination Voltammetry of Adenine and Cytosine Mixtures

Autoři

TRNKOVÁ, Libuše; Jiří FRIML a Oldřich DRAČKA

Vydání

Bioelectrochemistry, The Netherlands, Elsevier, 2001, 1567-5394

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10405 Electrochemistry

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.096

Kód RIV

RIV/00216224:14310/01:00005416

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Elimination voltammetry with linear scan; cytosine; adenine; reduction of bases; hydrogen evolution

Štítky

adenine, cytosine, hydrogen evolution, reduction of bases

Změněno: 25. 1. 2002 21:10, prof. RNDr. Libuše Trnková, CSc.

Anotace

V originále

The elimination voltammetry with linear scan (EVLS) was used to study adenine and cytosine reduction signals at the mercury electrode. In comparison with the linear scan voltammetry providing only one unresolved peak, two elimination functions provide good resolution of individual peaks and significant increase of sensitivity.Two selected functions give two well resolved peaks of adenine and cytosine in a wide concentration range, while the linear sweep voltammetry gives badly resolved peaks due to the hydrogen evolution. The best resolution of peaks is observed in acetate buffer at pH 3.8 and a detection limit for both substances is 500 nM. The concentration dependence of EVLS peak heights for one substance at the constant concentration of the other substance is linear. The peak potentials differ in these elimination functions. The difference in EVLS peak potentials gives the possibility to evaluate .EVLS contributes to the resolution of cathodic signals of purine and pyrimidine bases at very negative potentials near supporting electrolyte discharge.

Návaznosti

GV204/97/K084, projekt VaV

Název: Elektrody modifikované nukleovými kyselinami a bílkovinami. Nové nástroje v biochemickém a biomedicínském výzkumu

Investor: Grantová agentura ČR, Elektrody modifikované nukleovými kyselinami a bílkovinami. Nové nástroje v biochemickém a biomedicínském výzkumu

MSM 143100011, záměr

Název: Struktura a vazebné poměry, vlastnosti a analýza syntetických a přírodních molekulových ansamblů

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Struktura a vazebné poměry, vlastnosti a analýza syntetických a přírodních molekulových ansamblů

Citovat

TRNKOVÁ, Libuše; Jiří FRIML a Oldřich DRAČKA. Elimination Voltammetry of Adenine and Cytosine Mixtures. Bioelectrochemistry. The Netherlands: Elsevier, 2001, roč. 2001, č. 54, s. 131-136. ISSN 1567-5394.

@article{390892,
   author = {Trnková, Libuše and Friml, Jiří and Dračka, Oldřich},
   article_location = {The Netherlands},
   article_number = {54},
   keywords = {Elimination voltammetry with linear scan; cytosine; adenine; reduction of bases; hydrogen evolution},
   language = {eng},
   issn = {1567-5394},
   journal = {Bioelectrochemistry},
   title = {Elimination Voltammetry of Adenine and Cytosine Mixtures},
   volume = {2001},
   year = {2001}
}

TY  - JOUR
ID  - 390892
AU  - Trnková, Libuše - Friml, Jiří - Dračka, Oldřich
PY  - 2001
TI  - Elimination Voltammetry of Adenine and Cytosine Mixtures
JF  - Bioelectrochemistry
VL  - 2001
IS  - 54
SP  - 131
EP  - 131
PB  - Elsevier
SN  - 15675394
KW  - Elimination voltammetry with linear scan
KW  - cytosine
KW  - adenine
KW  - reduction of bases
KW  - hydrogen evolution
N2  - The elimination voltammetry with linear scan (EVLS) was used to study adenine and cytosine reduction signals at the mercury electrode. In comparison with the linear scan voltammetry providing only one unresolved peak, two elimination functions provide good resolution of individual peaks and significant increase of sensitivity.Two selected functions give two well resolved peaks of adenine and cytosine in a wide concentration range, while the linear sweep voltammetry gives badly resolved peaks due to the hydrogen evolution. The best resolution of peaks is observed in acetate buffer at pH 3.8 and a detection limit for both substances is 500 nM. The concentration dependence of EVLS peak heights for one substance at the constant concentration of the other substance is linear. The peak potentials differ in these elimination functions. The difference in EVLS peak potentials gives the possibility to evaluate .EVLS contributes to the resolution of cathodic signals of purine and pyrimidine bases at very negative potentials near supporting electrolyte discharge.
ER  -

TRNKOVÁ, Libuše; Jiří FRIML a Oldřich DRAČKA. Elimination Voltammetry of Adenine and Cytosine Mixtures. \textit{Bioelectrochemistry}. The Netherlands: Elsevier, 2001, roč.~2001, č.~54, s.~131-136. ISSN~1567-5394.

Přehled o publikaci