Silver Electrode as a Sensor for Determination of Zinc in Cell
Cultivation Medium

J 2002

Silver Electrode as a Sensor for Determination of Zinc in Cell Cultivation Medium

KIZEK, René, Libuše TRNKOVÁ, Sabina ŠEVČÍKOVÁ, Jan ŠMARDA, František JELEN et. al.

Základní údaje

Originální název

Silver Electrode as a Sensor for Determination of Zinc in Cell Cultivation Medium

Autoři

KIZEK, René (203 Česká republika), Libuše TRNKOVÁ (203 Česká republika, garant), Sabina ŠEVČÍKOVÁ (203 Česká republika), Jan ŠMARDA (203 Česká republika) a František JELEN (203 Česká republika)

Vydání

Analytical Biochemistry, San Diego, Academic Press, 2002, 0003-2697

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10405 Electrochemistry

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.370

Kód RIV

RIV/00216224:14310/02:00005423

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000173661300002

Klíčová slova anglicky

silver electrode; cyclic voltammetry; zinc in growth medium; inducible gene expression; BM2 cells; immunoblotting

Štítky

BM2 cells, cyclic voltammetry, immunoblotting, inducible gene expression, silver electrode, zinc in growth medium

Změněno: 8. 10. 2003 10:30, prof. RNDr. Libuše Trnková, CSc.

Anotace

V originále

Use of the silver electrode as a sensor for monitoring of zinc in cell growth medium is described. Zinc at siver electrodes provides specific voltammetric signal, which is affected by solution components. Signlas of zinc ions in phosphate buffer solutions with and without cell growth meidum were compared. Common DMEM cell culture medium was used for the cultivation of a cell line of v-myb-transformed chicken monoblasts and its variants expressing v-jun and c-jun in a zinc dependent manner. Electrochemical results showed zinc concentrations in the medium coincide very well with the jun expression. With respect to the low toxicity of xilver for eukaryotic cells, silver electrodes represent promising tools for the determination of zinc concentrations in vivo without the potential risk of a cell culture damage.

Návaznosti

GV204/97/K084, projekt VaV

Název: Elektrody modifikované nukleovými kyselinami a bílkovinami. Nové nástroje v biochemickém a biomedicínském výzkumu

Investor: Grantová agentura ČR, Elektrody modifikované nukleovými kyselinami a bílkovinami. Nové nástroje v biochemickém a biomedicínském výzkumu

MSM 143100008, záměr

Název: Genomy a jejich funkce

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Genomy a jejich funkce

Citovat

KIZEK, René, Libuše TRNKOVÁ, Sabina ŠEVČÍKOVÁ, Jan ŠMARDA a František JELEN. Silver Electrode as a Sensor for Determination of Zinc in Cell Cultivation Medium. Analytical Biochemistry. San Diego: Academic Press, 2002, roč. 301, č. 2002, s. 8-13. ISSN 0003-2697.

@article{390991,
   author = {Kizek, René and Trnková, Libuše and Ševčíková, Sabina and Šmarda, Jan and Jelen, František},
   article_location = {San Diego},
   article_number = {2002},
   keywords = {silver electrode; cyclic voltammetry; zinc in growth medium; inducible gene expression; BM2 cells; immunoblotting},
   language = {eng},
   issn = {0003-2697},
   journal = {Analytical Biochemistry},
   title = {Silver Electrode as a Sensor for Determination of Zinc in Cell Cultivation Medium},
   volume = {301},
   year = {2002}
}

TY  - JOUR
ID  - 390991
AU  - Kizek, René - Trnková, Libuše - Ševčíková, Sabina - Šmarda, Jan - Jelen, František
PY  - 2002
TI  - Silver Electrode as a Sensor for Determination of Zinc in Cell Cultivation Medium
JF  - Analytical Biochemistry
VL  - 301
IS  - 2002
SP  - 8
EP  - 8
PB  - Academic Press
SN  - 00032697
KW  - silver electrode
KW  - cyclic voltammetry
KW  - zinc in growth medium
KW  - inducible gene expression
KW  - BM2 cells
KW  - immunoblotting
N2  - Use of the silver electrode as a sensor for monitoring of zinc in cell growth medium is described. Zinc at siver electrodes provides specific voltammetric signal, which is affected by solution components. Signlas of zinc ions in phosphate buffer solutions with and without cell growth meidum were compared. Common DMEM cell culture medium was used for the cultivation of a cell line of v-myb-transformed chicken monoblasts and its variants expressing v-jun and c-jun in a zinc dependent manner. Electrochemical results showed zinc concentrations in the medium coincide very well with the jun expression. With respect to the low toxicity of xilver for eukaryotic cells, silver electrodes represent promising tools for the determination of zinc concentrations in vivo without the potential risk of a cell culture damage.
ER  -

KIZEK, René, Libuše TRNKOVÁ, Sabina ŠEVČÍKOVÁ, Jan ŠMARDA a František JELEN. Silver Electrode as a Sensor for Determination of Zinc in Cell Cultivation Medium. \textit{Analytical Biochemistry}. San Diego: Academic Press, 2002, roč.~301, č.~2002, s.~8-13. ISSN~0003-2697.

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