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@inproceedings{404626,
author = {Skalníková, Magdalena and Kozubek, Michal and Matula, Petr and Bártová, Eva and Eipel, Heinz and Hausmann, Michael},
address = {Brno},
booktitle = {Biophysics of the Genome and Its Interactions},
keywords = {Chromatin structure; FISH; Micro axial tomography; High-resolution cytometry; fibre glass},
language = {eng},
location = {Brno},
isbn = {80-210-2853-X},
pages = {82-85},
publisher = {Masaryk University},
title = {Optimisation of FISH Methodology for the Study of 3D Structure of Cell Nuclei Using Micro Axial Tomography},
year = {2002}
}
TY - JOUR
ID - 404626
AU - Skalníková, Magdalena - Kozubek, Michal - Matula, Petr - Bártová, Eva - Eipel, Heinz - Hausmann, Michael
PY - 2002
TI - Optimisation of FISH Methodology for the Study of 3D Structure of Cell Nuclei Using Micro Axial Tomography
PB - Masaryk University
CY - Brno
SN - 802102853X
KW - Chromatin structure
KW - FISH
KW - Micro axial tomography
KW - High-resolution cytometry
KW - fibre glass
N2 - Micro axial tomography is a new method for microscopic image acquisition of fluorescence in situ hybridisation (FISH) stained cell nuclei with improved spatial resolution. Automated axial tomography has grown up from a common Brno-Heidelberg collaboration of two scientific groups, which are interested in the study of higher order chromatin structure in FISH stained cells using epifluorescence and confocal laser scanning fluorescence microscopy. The aim of the common Brno-Heidelberg project is to combine high-resolution cytometry (HRCM) and 2đ-tilting device in order to obtain statistically significant data about the structure of cell nuclei using advanced microscopy and fast automated computer image acquisition and analysis. In this study a method for the preparation of cell nuclei attached to glass fibres has been developed. The optimised experimental procedure for the first time provides imaging of three-dimensionally conserved, FISH-stained cell nuclei fixed to a glass fibre
ER -
SKALNÍKOVÁ, Magdalena, Michal KOZUBEK, Petr MATULA, Eva BÁRTOVÁ, Heinz EIPEL a Michael HAUSMANN. Optimisation of FISH Methodology for the Study of 3D Structure of Cell Nuclei Using Micro Axial Tomography. In \textit{Biophysics of the Genome and Its Interactions}. Brno: Masaryk University, 2002, s.~82-85. ISBN~80-210-2853-X.
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