Impedance analysis of DNA and DNA-drug interactions on thin
mercury film electrodes.

J 2002

Impedance analysis of DNA and DNA-drug interactions on thin mercury film electrodes.

HASOŇ, Stanislav, Jakub DVOŘÁK, František JELEN a Vladimír VETTERL

Základní údaje

Originální název

Impedance analysis of DNA and DNA-drug interactions on thin mercury film electrodes.

Autoři

HASOŇ, Stanislav (203 Česká republika), Jakub DVOŘÁK (203 Česká republika), František JELEN (203 Česká republika) a Vladimír VETTERL (203 Česká republika, garant)

Vydání

Critical Reviews in Analytical Chemistry, Boca Raton (Florida, USA), CRC Press, 2002, 1040-8347

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10610 Biophysics

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.074

Kód RIV

RIV/00216224:14310/02:00007363

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

electrochemical impedance spectroscopy; EIS; DNA adsorption; echinomycin; DNA-echinomycin interaction; mercury film electrodes;

Štítky

DNA adsorption, DNA-echinomycin interaction, echinomycin, EIS, electrochemical impedance spectroscopy, mercury film electrodes

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 30. 6. 2008 14:16, prof. RNDr. Vladimír Vetterl, DrSc.

Anotace

V originále

Electrochemical behaviour of echinomycin, DNA and DNA-echinomycin complex at the mercury film electrodes (MFE) was studied by measurement of the impedance of the electrode double layer (dependence of the differential capacitance C on the electrode potential, C-E curves, and dependence of the impedance Z on ferquency - electrochemical impedance spectroscopy, EIS). Mercury film was plated either on a glassy carbonelectrode (GCE) or pyrolytic graphite electrode (PGE). The capacitance measurement showed that echinomycin forms the pseudocapacitance redox peak around potential -0.53 V at the MFE.Interaction of single stranded (ss) and double stranded (ds) DNA with echinomycin at DNA-modified electrode surface was studied using EIS with adsorptive transfer method. Pseudocapacitance peak of echinomycin was observed with dsDNA-echinomycin complex, but not with ssDNA-echinomycin comlex. This result is in agreement with the known strong binding of echinomycin to dsDNA by bis-intercalation and only very weak interactin of echinomycin with ssDNA.

Návaznosti

GV204/97/K084, projekt VaV

Název: Elektrody modifikované nukleovými kyselinami a bílkovinami. Nové nástroje v biochemickém a biomedicínském výzkumu

Investor: Grantová agentura ČR, Elektrody modifikované nukleovými kyselinami a bílkovinami. Nové nástroje v biochemickém a biomedicínském výzkumu

IAA4004002, projekt VaV

Název: Struktura a interakce nukleových kyselin a polypeptidů na površích kovů

Investor: Akademie věd ČR, Struktura a interakce nukleových kyselin a polypeptidů na površích kovů

Citovat

HASOŇ, Stanislav, Jakub DVOŘÁK, František JELEN a Vladimír VETTERL. Impedance analysis of DNA and DNA-drug interactions on thin mercury film electrodes. Critical Reviews in Analytical Chemistry. Boca Raton (Florida, USA): CRC Press, 2002, roč. 32, č. 2, s. 167-179. ISSN 1040-8347.

@article{483763,
   author = {Hasoň, Stanislav and Dvořák, Jakub and Jelen, František and Vetterl, Vladimír},
   article_location = {Boca Raton (Florida, USA)},
   article_number = {2},
   keywords = {electrochemical impedance spectroscopy; EIS; DNA adsorption; echinomycin; DNA-echinomycin interaction; mercury film electrodes;},
   language = {eng},
   issn = {1040-8347},
   journal = {Critical Reviews in Analytical Chemistry},
   title = {Impedance analysis of DNA and DNA-drug interactions on thin mercury film electrodes.},
   volume = {32},
   year = {2002}
}

TY  - JOUR
ID  - 483763
AU  - Hasoň, Stanislav - Dvořák, Jakub - Jelen, František - Vetterl, Vladimír
PY  - 2002
TI  - Impedance analysis of DNA and DNA-drug interactions on thin mercury film electrodes.
JF  - Critical Reviews in Analytical Chemistry
VL  - 32
IS  - 2
SP  - 167
EP  - 167
PB  - CRC Press
SN  - 10408347
KW  - electrochemical impedance spectroscopy
KW  - EIS
KW  - DNA adsorption
KW  - echinomycin
KW  - DNA-echinomycin interaction
KW  - mercury film electrodes;
N2  - Electrochemical behaviour of echinomycin, DNA and DNA-echinomycin complex at the mercury film electrodes (MFE) was studied by measurement of the impedance of the electrode double layer (dependence of the differential capacitance C on the electrode potential, C-E curves, and dependence of the impedance Z on ferquency - electrochemical impedance spectroscopy, EIS). Mercury film was plated either on a glassy carbonelectrode (GCE) or pyrolytic graphite electrode (PGE). The capacitance measurement showed that echinomycin forms the pseudocapacitance redox peak around potential -0.53 V at the MFE.Interaction of single stranded (ss) and double stranded (ds) DNA with echinomycin at DNA-modified electrode surface was studied using EIS with adsorptive transfer method. Pseudocapacitance peak of echinomycin was observed with dsDNA-echinomycin complex, but not with ssDNA-echinomycin comlex. This result is in agreement with the known strong binding of echinomycin to dsDNA by bis-intercalation and only very weak interactin of echinomycin with ssDNA.
ER  -

HASOŇ, Stanislav, Jakub DVOŘÁK, František JELEN a Vladimír VETTERL. Impedance analysis of DNA and DNA-drug interactions on thin mercury film electrodes. \textit{Critical Reviews in Analytical Chemistry}. Boca Raton (Florida, USA): CRC Press, 2002, roč.~32, č.~2, s.~167-179. ISSN~1040-8347.

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