Electrochemical Detection of Interactions of Echinomycin with
DNA at Mercury Film Electrodes (MFE).

D 2001

Electrochemical Detection of Interactions of Echinomycin with DNA at Mercury Film Electrodes (MFE).

HASOŇ, Stanislav, Jakub DVOŘÁK, František JELEN a Vladimír VETTERL

Základní údaje

Originální název

Electrochemical Detection of Interactions of Echinomycin with DNA at Mercury Film Electrodes (MFE).

Autoři

HASOŇ, Stanislav (203 Česká republika), Jakub DVOŘÁK (203 Česká republika), František JELEN (203 Česká republika) a Vladimír VETTERL (203 Česká republika, garant)

Vydání

Prague, Book of Abstracts, US-CZ Workshops on Electrochemical Sensors Prague 2001, s. 42-42, 2001

Nakladatel

US-CZ Workshop on Electrochemical Sensors Prague

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10610 Biophysics

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/01:00007523

Organizační jednotka

Přírodovědecká fakulta

ISBN

80-86238-15-6

Klíčová slova anglicky

DNA-echinomycin interaction; electrochemical impedance spectroscopy;EIS; DNA adsorption; mercuiry film electrodes;

Štítky

DNA adsorption, DNA-echinomycin interaction, EIS, electrochemical impedance spectroscopy, mercuiry film electrodes

Změněno: 10. 2. 2003 17:33, prof. RNDr. Vladimír Vetterl, DrSc.

Anotace

V originále

The dependence of the impedance Z and/or differential capacitance C of the electrode double layer on electrode potential E and on frequency in solutions of echinomycin with single stranded (ss) and double-helical (double stranded, ds) DNA was measured. Echinomycin gives a pseudocapacitance redox peak on the C-E curves at the potential of -0.53 V. This peak is observed with the dsDNA-echinomycin comlex as well, but not with ssDNA treated by echinomycin. The capacitance measurements can thus distinguish with the aid of bis-intercalator echinomycin the ss and ds form of DNA adsorbed at the electrode surface. This promise to use impedance measurements for detection of dsDNA in hybridization reactins.

Návaznosti

GV204/97/K084, projekt VaV

Název: Elektrody modifikované nukleovými kyselinami a bílkovinami. Nové nástroje v biochemickém a biomedicínském výzkumu

Investor: Grantová agentura ČR, Elektrody modifikované nukleovými kyselinami a bílkovinami. Nové nástroje v biochemickém a biomedicínském výzkumu

IAA4004002, projekt VaV

Název: Struktura a interakce nukleových kyselin a polypeptidů na površích kovů

Investor: Akademie věd ČR, Struktura a interakce nukleových kyselin a polypeptidů na površích kovů

Citovat

HASOŇ, Stanislav, Jakub DVOŘÁK, František JELEN a Vladimír VETTERL. Electrochemical Detection of Interactions of Echinomycin with DNA at Mercury Film Electrodes (MFE). In Book of Abstracts, US-CZ Workshops on Electrochemical Sensors Prague 2001. Prague: US-CZ Workshop on Electrochemical Sensors Prague, 2001, s. 42. ISBN 80-86238-15-6.

@inproceedings{484360,
   author = {Hasoň, Stanislav and Dvořák, Jakub and Jelen, František and Vetterl, Vladimír},
   address = {Prague},
   booktitle = {Book of Abstracts, US-CZ Workshops on Electrochemical Sensors Prague 2001},
   keywords = {DNA-echinomycin interaction; electrochemical impedance spectroscopy;EIS; DNA adsorption; mercuiry film electrodes;},
   language = {eng},
   location = {Prague},
   isbn = {80-86238-15-6},
   pages = {42-42},
   publisher = {US-CZ Workshop on Electrochemical Sensors Prague},
   title = {Electrochemical Detection of Interactions of Echinomycin with DNA at Mercury Film Electrodes (MFE).},
   year = {2001}
}

TY  - JOUR
ID  - 484360
AU  - Hasoň, Stanislav - Dvořák, Jakub - Jelen, František - Vetterl, Vladimír
PY  - 2001
TI  - Electrochemical Detection of Interactions of Echinomycin with DNA at Mercury Film Electrodes (MFE).
PB  - US-CZ Workshop on Electrochemical Sensors Prague
CY  - Prague
SN  - 8086238156
KW  - DNA-echinomycin interaction
KW  - electrochemical impedance spectroscopy;EIS
KW  - DNA adsorption
KW  - mercuiry film electrodes;
N2  - The dependence of the impedance Z and/or differential capacitance C of the electrode double layer on electrode potential E and on frequency in solutions of echinomycin with single stranded (ss) and double-helical (double stranded, ds) DNA was measured. Echinomycin gives a pseudocapacitance redox peak on the C-E curves at the potential of -0.53 V. This peak is observed with the dsDNA-echinomycin comlex as well, but not with ssDNA treated by echinomycin. The capacitance measurements can thus distinguish with the aid of bis-intercalator echinomycin the ss and ds form of DNA adsorbed at the electrode surface. This promise to use impedance measurements for detection of dsDNA in hybridization reactins.
ER  -

HASOŇ, Stanislav, Jakub DVOŘÁK, František JELEN a Vladimír VETTERL. Electrochemical Detection of Interactions of Echinomycin with DNA at Mercury Film Electrodes (MFE). In \textit{Book of Abstracts, US-CZ Workshops on Electrochemical Sensors Prague 2001}. Prague: US-CZ Workshop on Electrochemical Sensors Prague, 2001, s.~42. ISBN~80-86238-15-6.

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