Square wave voltammetric analysis of azidothymidine in the
presence of oligonucleotides and chromosomal DNA

D 2003

Square wave voltammetric analysis of azidothymidine in the presence of oligonucleotides and chromosomal DNA

TRNKOVÁ, Libuše; René KIZEK a Jan VACEK

Základní údaje

Originální název

Square wave voltammetric analysis of azidothymidine in the presence of oligonucleotides and chromosomal DNA

Autoři

TRNKOVÁ, Libuše (203 Česká republika, garant); René KIZEK (203 Česká republika) a Jan VACEK (203 Česká republika)

Vydání

1. vyd. Florence, Italy, XVII th International Symposium on Bioelectrochemictry and Bioenergetics, s. 139-139, 2003

Nakladatel

The Bioelectrochemical Society

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10405 Electrochemistry

Stát vydavatele

Itálie

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/03:00008112

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

azidothymidine; hanging mercury drop electrode; square wave voltammetry; elimination voltammetry with linear scan; oligonucleotides; chromosomal DNA

Štítky

azidothymidine, chromosomal DNA, hanging mercury drop electrode, Oligonucleotides, square wave voltammetry

Změněno: 20. 6. 2008 12:00, prof. RNDr. Libuše Trnková, CSc.

Anotace

V originále

Azidothymidine ( AZT, Zidovudine) is a synthetic nucleoside analogue with activity against human immunodeficiency virus type 1 (HIV). Recently, our working group has developed and optimised a square wave voltammetric method for analysis of AZT in biological samples. The aim of the study is the determination of AZT in the presence of synthetic oligonucleotides (ODNs) and calf thymus ssDNA or dsDNA on the hanging mercury drop electrode. Both AZT and DNA yield voltammetric signals on the mercury electrode; AZT provides an irreversible reduction peak at the potential near -1.1 V (vs. Ag/AgCl/3M KCl) and DNA provides a reduction peak of adenine and cytosine at the potential ca. -1.4 V. Square wave voltammetric signals of AZT and DNA were measured in phosphate buffer (pH 8.0) at a frequency of 2000 Hz and accumulation time of 10 s. We found that AZT peak height is not affected by the presence of DNA and therefore we assume that electron transfer proceeds through the adsorption layer of ssDNA and/or dsDNA. The detection limit of AZT is 1 nM and 250 nM in absence and in presence of ssDNA (10 mg/mL), respectively. The SWV is an effective tool for the AZT determination incorporated into oligonucleotide chains. This promising methodology ca be used to study AZT cytotoxicity and kinetics of AZT metabolism in HIV positive patients.

Návaznosti

GA203/02/0422, projekt VaV

Název: Nové směry v elektrochemii nukleových kyselin a jejich aplikace v chemii životního prostředí

Investor: Grantová agentura ČR, Nové směry v elektrochemii nukleových kyselin a jejich aplikace v chemii životního prostředí

IAA1163201, projekt VaV

Název: Využití adsorptivní přenosové a eliminační techniky pro elektrochemickou analýzu oligonukleotidů a nukleových kyselin

Investor: Akademie věd ČR, Využití adsorptivní přenosové a eliminační techniky pro elektrochemickou analýzu oligonukleotidů a nukleových kyselin

Citovat

TRNKOVÁ, Libuše; René KIZEK a Jan VACEK. Square wave voltammetric analysis of azidothymidine in the presence of oligonucleotides and chromosomal DNA. In XVII th International Symposium on Bioelectrochemictry and Bioenergetics. 1. vyd. Florence, Italy: The Bioelectrochemical Society, 2003, s. 139.

@inproceedings{487724,
   author = {Trnková, Libuše and Kizek, René and Vacek, Jan},
   address = {Florence, Italy},
   booktitle = {XVII th International Symposium on Bioelectrochemictry and Bioenergetics},
   edition = {1},
   keywords = {azidothymidine; hanging mercury drop electrode; square wave voltammetry; elimination voltammetry with linear scan; oligonucleotides; chromosomal DNA},
   language = {eng},
   location = {Florence, Italy},
   pages = {139-139},
   publisher = {The Bioelectrochemical Society},
   title = {Square wave voltammetric analysis of azidothymidine in the presence of oligonucleotides and chromosomal DNA},
   year = {2003}
}

TY  - CONF
ID  - 487724
AU  - Trnková, Libuše - Kizek, René - Vacek, Jan
PY  - 2003
TI  - Square wave voltammetric analysis of azidothymidine in the presence of oligonucleotides and chromosomal DNA
PB  - The Bioelectrochemical Society
CY  - Florence, Italy
KW  - azidothymidine
KW  - hanging mercury drop electrode
KW  - square wave voltammetry
KW  - elimination voltammetry with linear scan
KW  - oligonucleotides
KW  - chromosomal DNA
N2  - Azidothymidine ( AZT, Zidovudine) is a synthetic nucleoside analogue with activity against human immunodeficiency virus type 1 (HIV). Recently, our working group has developed and optimised a square wave voltammetric method for analysis of AZT in biological samples. The aim of the study is the determination of AZT in the presence of synthetic oligonucleotides (ODNs) and calf thymus ssDNA or dsDNA on the hanging mercury drop electrode. Both AZT and DNA yield voltammetric signals on the mercury electrode; AZT provides an irreversible reduction peak at the potential near -1.1 V (vs. Ag/AgCl/3M KCl) and DNA provides a reduction peak of adenine and cytosine at the potential ca. -1.4 V. Square wave voltammetric signals of AZT and DNA were measured in phosphate buffer (pH 8.0) at a frequency of 2000 Hz and accumulation time of 10 s. We found that AZT peak height is not affected by the presence of DNA and therefore we assume that electron transfer proceeds through the adsorption layer of ssDNA and/or dsDNA. The detection limit of AZT is 1 nM and 250 nM in absence and in presence of ssDNA (10 mg/mL), respectively. The SWV is an effective tool for the AZT determination incorporated into oligonucleotide chains. This promising methodology ca be used to study AZT cytotoxicity and kinetics of AZT metabolism in HIV positive patients.
ER  -

TRNKOVÁ, Libuše; René KIZEK a Jan VACEK. Square wave voltammetric analysis of azidothymidine in the presence of oligonucleotides and chromosomal DNA. In \textit{XVII th International Symposium on Bioelectrochemictry and Bioenergetics}. 1. vyd. Florence, Italy: The Bioelectrochemical Society, 2003, s.~139.

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