| MASAŘÍK, Michal, René KIZEK, K.J. KRAMER, Sabina BILLOVÁ, Marie BRÁZDOVÁ, Jan VACEK, Michele BAILEY, František JELEN and A. J. HOWARD. Application of avidin-biotin technology and adsorptive transfer stripping square-wave voltammetry for detection of DNA hybridization and avidin in transgenic avidin maize. Analytical Chemistry. Washington, D.C., USA: American Chemical Society, 2003, vol. 75, No 11, p. 2663-2669. ISSN 0003-2700. |
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@article{489639,
author = {Masařík, Michal and Kizek, René and Kramer, K.J. and Billová, Sabina and Brázdová, Marie and Vacek, Jan and Bailey, Michele and Jelen, František and Howard, A. J.},
article_location = {Washington, D.C., USA},
article_number = {11},
keywords = {Streptavidin; Avidin; Adorptive Transfer Stripping Voltammetry; Square-Wave Voltammetry; Biotin Binding; Transgenic Avidin Maize; Corn; Grain; Transgenic Protein Detection; Electrochemical Detection; DNA Hybridization},
language = {eng},
issn = {0003-2700},
journal = {Analytical Chemistry},
title = {Application of avidin-biotin technology and adsorptive transfer stripping square-wave voltammetry for detection of DNA hybridization and avidin in transgenic avidin maize},
volume = {75},
year = {2003}
}
TY - JOUR
ID - 489639
AU - Masařík, Michal - Kizek, René - Kramer, K.J. - Billová, Sabina - Brázdová, Marie - Vacek, Jan - Bailey, Michele - Jelen, František - Howard, A. J.
PY - 2003
TI - Application of avidin-biotin technology and adsorptive transfer stripping square-wave voltammetry for detection of DNA hybridization and avidin in transgenic avidin maize
JF - Analytical Chemistry
VL - 75
IS - 11
SP - 2663-2669
EP - 2663-2669
PB - American Chemical Society
SN - 00032700
KW - Streptavidin
KW - Avidin
KW - Adorptive Transfer Stripping Voltammetry
KW - Square-Wave Voltammetry
KW - Biotin Binding
KW - Transgenic Avidin Maize
KW - Corn
KW - Grain
KW - Transgenic Protein Detection
KW - Electrochemical Detection
KW - DNA Hybridization
N2 - The proteins streptavidin and avidin were electrochemically detected in solution by adsorptive transfer stripping square wave voltammetry (AdTS SWV) at a carbon paste electrode (CPE). AdTS SWV was used to quantify biotinylated oligonucleotides, DNA hybridizations, and avidin in extracts of transgenic avidin maize. The detection limits of denatured and native streptavidin were 6 pM and 120 nM, respectively. The results demonstrated that streptavidin/avidin AdTS SWV is a sensitive and specific method for quatifying DNA and proteins in biological samples such as foods and tissue extracts, including genetically modified crops (avidin maize) and other plants in neighboring fields.
ER -
MASAŘÍK, Michal, René KIZEK, K.J. KRAMER, Sabina BILLOVÁ, Marie BRÁZDOVÁ, Jan VACEK, Michele BAILEY, František JELEN and A. J. HOWARD. Application of avidin-biotin technology and adsorptive transfer stripping square-wave voltammetry for detection of DNA hybridization and avidin in transgenic avidin maize. \textit{Analytical Chemistry}. Washington, D.C., USA: American Chemical Society, 2003, vol.~75, No~11, p.~2663-2669. ISSN~0003-2700.
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