2003
Differential effects of transcription coactivators CBP and p300 on v-myb-transfromed monoblasts
NEMAJEROVÁ, Alice; Jan ŠMARDA; Jiřina ZATLOUKALOVÁ a Jana ŠMARDOVÁZákladní údaje
Originální název
Differential effects of transcription coactivators CBP and p300 on v-myb-transfromed monoblasts
Název anglicky
Differential effects of transcription coactivators CBP and p300 on v-myb-transfromed monoblasts
Autoři
Vydání
Brno, Memory in Living Systems, s. 66-66, 2003
Nakladatel
Czechoslovak Biological Society
Další údaje
Jazyk
čeština
Typ výsledku
Stať ve sborníku
Obor
Genetika a molekulární biologie
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Označené pro přenos do RIV
Ano
Kód RIV
RIV/00216224:14310/03:00009569
Organizační jednotka
Přírodovědecká fakulta
ISBN
80-210-3264-2
Klíčová slova anglicky
CBP; p300; Myb; histone acetylation
Štítky
Změněno: 23. 4. 2004 10:33, prof. RNDr. Jan Šmarda, CSc.
V originále
The v-myb oncogene is efficiently expressed in BM2 monoblasts thus blocking their terminal differentiation steps. Histone acetylation plays an important role in regulation of transcription and is particularly relevant to regulation and pathology of hematopoiesis. Recently, we have shown that inhibitor of histone deacetylases trichostatin A (TSA) causes histone hyperacetylation and differentiation of BM2 cells into macrophage polycaryons. In this work we used another approach to stimulate histone acetylation in BM2 cells. We prepared two variants of BM2 cells ectopically expressing two histone acetyl transferases CBP and p300. The expression of CBP as well as p300 itself did not affect the phenotype of BM2 cells, but it enhanced their response to the differentiation inducers TPA and TSA. Although CBP and p300 are closely related proteins, many differences in their function have been described. We noted differences in kinetics of differentiation induced by CBP and p300 in BM2 cells.
Anglicky
The v-myb oncogene is efficiently expressed in BM2 monoblasts thus blocking their terminal differentiation steps. Histone acetylation plays an important role in regulation of transcription and is particularly relevant to regulation and pathology of hematopoiesis. Recently, we have shown that inhibitor of histone deacetylases trichostatin A (TSA) causes histone hyperacetylation and differentiation of BM2 cells into macrophage polycaryons. In this work we used another approach to stimulate histone acetylation in BM2 cells. We prepared two variants of BM2 cells ectopically expressing two histone acetyl transferases CBP and p300. The expression of CBP as well as p300 itself did not affect the phenotype of BM2 cells, but it enhanced their response to the differentiation inducers TPA and TSA. Although CBP and p300 are closely related proteins, many differences in their function have been described. We noted differences in kinetics of differentiation induced by CBP and p300 in BM2 cells.
Návaznosti
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