Surface carbohydrates of Eudiplozoon nipponicum pre- and
post-fusion

J 2004

Surface carbohydrates of Eudiplozoon nipponicum pre- and post-fusion

SCHABUSSOVA, Irma; Božena KOUBKOVÁ; Milan GELNAR; Michael SCHABUSS; Petr HORÁK et al.

Základní údaje

Originální název

Surface carbohydrates of Eudiplozoon nipponicum pre- and post-fusion

Název česky

Povrchové karbohydráty Eudiplozoon nipponicum před a po párování

Autoři

SCHABUSSOVA, Irma; Božena KOUBKOVÁ; Milan GELNAR; Michael SCHABUSS a Petr HORÁK

Vydání

Journal of Helminthology, Wallingford, UK, CABI Publishing, 2004, 0022-149X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 0.676

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/04:00010185

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000220488300010

Klíčová slova anglicky

SCHISTOSOMA-MANSONI; LECTINS

Štítky

Lectins, SCHISTOSOMA-MANSONI

Změněno: 31. 5. 2005 13:49, RNDr. Božena Koubková, Ph.D.

Anotace

ORIG CZ

V originále

The development of the monogenean Diplozoon (Nordmann, 1832) (Diplozoidae) necessitates fusion of two larval stages (diporpae) into one double organism. How diporpae find, distinguish and contact each other is unclear, nor is the nature of the stimuli responsible for the dedifferentiation of cells and the formation of new tissues at the site of somatic fusion. Previous studies have implied a role for carbohydrates and glycoproteins in the interactions between helminth parasites and their hosts. Hypothetically, glycoconjugates may also be involved in the establishment of parasite-parasite associations. Changes in the surface saccharide residues during the development of Eudiplozoon nipponicum, a gill ectoparasite of carp (Cyprinus carpio) are described. Flat-fixed specimens and sections of diporpae, juveniles (just-fused) and adult worms were examined following exposure to a panel of 12 FITC-conjugated lectins. All developmental stages exhibited a specific surface binding pattern with ten lectins, indicating that Man/Glc, GlcNAc, Gal and GalNAc are probably present on their surfaces. No reaction was observed with Fuc-specific lectins (UEA-I and LTA). There is evidence that parasite development is accompanied by both qualitative and quantitative changes in the saccharide pattern distribution. The diporpa sucker reacted with nine lectins, excluding BS-II. A very strong binding of PNA, LCA and ConA (Gal and Man/Glc-specific lectins) was observed with the papilla glands of juvenile worms. The role of glandular secretions in this unique fusion process is discussed.

Česky

Jsou popsány změny povrchových sacharidů během vývoje Eudiplozoon nippnicum, žaberního paraziota kapra (Cyprinus carpio).

Návaznosti

MSM 143100010, záměr

Název: Časoprostorová dynamika biodiverzity v ekosystémech střední Evropy.

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Časoprostorová dynamika biodiverzity v ekosystémech střední Evropy

Citovat

SCHABUSSOVA, Irma; Božena KOUBKOVÁ; Milan GELNAR; Michael SCHABUSS a Petr HORÁK. Surface carbohydrates of Eudiplozoon nipponicum pre- and post-fusion. Journal of Helminthology. Wallingford, UK: CABI Publishing, 2004, roč. 78, č. 1, s. 63 - 68. ISSN 0022-149X.

@article{556408,
   author = {Schabussova, Irma and Koubková, Božena and Gelnar, Milan and Schabuss, Michael and Horák, Petr},
   article_location = {Wallingford, UK},
   article_number = {1},
   keywords = {SCHISTOSOMA-MANSONI; LECTINS},
   language = {eng},
   issn = {0022-149X},
   journal = {Journal of Helminthology},
   title = {Surface carbohydrates of Eudiplozoon nipponicum pre- and post-fusion},
   volume = {78},
   year = {2004}
}

TY  - JOUR
ID  - 556408
AU  - Schabussova, Irma - Koubková, Božena - Gelnar, Milan - Schabuss, Michael - Horák, Petr
PY  - 2004
TI  - Surface carbohydrates of Eudiplozoon nipponicum pre- and post-fusion
JF  - Journal of Helminthology
VL  - 78
IS  - 1
SP  - 63 - 68
EP  - 63 - 68
PB  - CABI Publishing
SN  - 0022149X
KW  - SCHISTOSOMA-MANSONI
KW  - LECTINS
N2  - The development of the monogenean Diplozoon (Nordmann, 1832) (Diplozoidae) necessitates fusion of two larval stages (diporpae) into one double organism. How diporpae find, distinguish and contact each other is unclear, nor is the nature of the stimuli responsible for the dedifferentiation of cells and the formation of new tissues at the site of somatic fusion. Previous studies have implied a role for carbohydrates and glycoproteins in the interactions between helminth parasites and their hosts. Hypothetically, glycoconjugates may also be involved in the establishment of parasite-parasite associations. Changes in the surface saccharide residues during the development of Eudiplozoon nipponicum, a gill ectoparasite of carp (Cyprinus carpio) are described. Flat-fixed specimens and sections of diporpae, juveniles (just-fused) and adult worms were examined following exposure to a panel of 12 FITC-conjugated lectins. All developmental stages exhibited a specific surface binding pattern with ten lectins, indicating that Man/Glc, GlcNAc, Gal and GalNAc are probably present on their surfaces. No reaction was observed with Fuc-specific lectins (UEA-I and LTA). There is evidence that parasite development is accompanied by both qualitative and quantitative changes in the saccharide pattern distribution. The diporpa sucker reacted with nine lectins, excluding BS-II. A very strong binding of PNA, LCA and ConA (Gal and Man/Glc-specific lectins) was observed with the papilla glands of juvenile worms. The role of glandular secretions in this unique fusion process is discussed.
ER  -

SCHABUSSOVA, Irma; Božena KOUBKOVÁ; Milan GELNAR; Michael SCHABUSS a Petr HORÁK. Surface carbohydrates of Eudiplozoon nipponicum pre- and post-fusion. \textit{Journal of Helminthology}. Wallingford, UK: CABI Publishing, 2004, roč.~78, č.~1, s.~63 - 68. ISSN~0022-149X.

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