Determination of Midazolam from Perfuse Medium as a Probe Substrate of Cytochrome P450 3A1/2 Activity in Rats

JUŘICA, Jan; Miroslav DOSTÁLEK; Josef TOMANDL a Eva HADAŠOVÁ. Determination of Midazolam from Perfuse Medium as a Probe Substrate of Cytochrome P450 3A1/2 Activity in Rats. In ACTA UNIVERISTATIS PALACKIANAE OLOMUCENSIS FACULTAS RERUM NATURALIUM. Olomouc: Univerzita Palackého Olomouc, 2004, s. 218-218. ISBN 80-244-0882-1.

Základní údaje

Originální název

Determination of Midazolam from Perfuse Medium as a Probe Substrate of Cytochrome P450 3A1/2 Activity in Rats

Název česky

STANOVENÍ MIDAZOLAMU Z PERFUZNÍHO MEDIA JAKO MARKERU CYTOCHROMU P450 3A1/2 U POTKANA

Název anglicky

DETERMINATION OF MIDAZOLAM FROM PERFUSE MEDIUM AS A PROBE SUBSTRATE OF CYTOCHROME P450 3A1/2 ACTIVITY IN RATS

Autoři

JUŘICA, Jan; Miroslav DOSTÁLEK; Josef TOMANDL ORCID a Eva HADAŠOVÁ

Vydání

Olomouc, ACTA UNIVERISTATIS PALACKIANAE OLOMUCENSIS FACULTAS RERUM NATURALIUM, od s. 218-218, 1 s. 2004

Nakladatel

Univerzita Palackého Olomouc

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Další údaje

Jazyk

čeština

Typ výsledku

Stať ve sborníku

Obor

30104 Pharmacology and pharmacy

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/04:00010735

Organizační jednotka

Lékařská fakulta

ISBN

80-244-0882-1

Klíčová slova anglicky

Midazolam; CYP-450

Štítky

CYP-450, midazolam

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Anotace

V originále

The aim of the project was to develop HPLC method for determination of midazolam and its three metabolites, 1- and 4-hydroxymidazolam and 1,4-dihydroxymidazolam from perfuse rat liver medium. Hydroxylation of midazolam is used as a valuable tool for measurement of metabolic activity of the 3A1/2 isoform of cytochrome P-450 in rats (CYP3A1/2)This method is simple, rapid, sensitive and applicable for modeling and description of possible pharmacological interactions on rat CYP3A1/2.

Anglicky

The aim of the project was to develop HPLC method for determination of midazolam and its three metabolites, 1- and 4-hydroxymidazolam and 1,4-dihydroxymidazolam from perfuse rat liver medium. Hydroxylation of midazolam is used as a valuable tool for measurement of metabolic activity of the 3A1/2 isoform of cytochrome P-450 in rats (CYP3A1/2)This method is simple, rapid, sensitive and applicable for modeling and description of possible pharmacological interactions on rat CYP3A1/2 or it`s human orthologue CYP3A4/5, respectively.