Refinement of Trypanosoma U6 RNA Stem-Loop Structure Using 1-
and 2-Bond Residual Dipolar Couplings

D 2004

Refinement of Trypanosoma U6 RNA Stem-Loop Structure Using 1- and 2-Bond Residual Dipolar Couplings

NOVÁK, Petr; Lukáš ŽÍDEK; Petr PADRTA a Vladimír SKLENÁŘ

Základní údaje

Originální název

Refinement of Trypanosoma U6 RNA Stem-Loop Structure Using 1- and 2-Bond Residual Dipolar Couplings

Název česky

Rafinace struktury Trypanosoma U6 RNA s využitím jedno- a dvouvazebných zbytkových dipolárních interakcí

Autoři

NOVÁK, Petr; Lukáš ŽÍDEK; Petr PADRTA a Vladimír SKLENÁŘ

Vydání

Brno, 19th NMR Valtice, od s. 30-30, 1 s. 2004

Nakladatel

Masarykova universita v Brně

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/04:00011133

Organizační jednotka

Přírodovědecká fakulta

ISBN

80-210-3352-5

Klíčová slova anglicky

NMR; Residual dipolar couplings; RNA; structure;

Štítky

NMR, residual dipolar couplings, RNA, structure

Změněno: 31. 5. 2005 13:42, Mgr. Petr Novák, Ph.D.

Anotace

ORIG CZ

V originále

The spliceosome is a complex of proteins and small nuclear RNAs (snRNAs) responsible for the removal of introns from pre-mRNA in eukaryotes. U6 snRNA has been demonstrated to be a vital element in splicing and its sequence is the most conserved of all snRNAs[1]. U6 snRNA from trypanosoma was our study. Determination of the solution structure of nucleic acid fragments is significantly improved if residual dipolar are measured. This data provides us additional long-range and local geometry restraints using a small degree of molecular alignment with the static magnetic field. We wanted to test polyethylenglycol (PEG) as a alignment medium for measuring the residual dipolar couplings (RDCs). PEG is a stable and unexpensive organic polymer that does not require a complex biochemical preparation like the most often used bacteriophage Pf1. In our study, applicability of PEG to several spin-state-selective E.COSY experiments was investigated. Measured RDCs were tested for internal consistency as refered in [2] in order to check the reliability of the values obtained in the PEG-aligned samples.

Česky

Rafinace struktury Trypanosoma U6 RNA s využitím jedno- a dvouvazebných zbytkových dipolárních interakcí.

Návaznosti

LN00A016, projekt VaV

Název: BIOMOLEKULÁRNÍ CENTRUM

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Biomolekulární centrum

Citovat

NOVÁK, Petr; Lukáš ŽÍDEK; Petr PADRTA a Vladimír SKLENÁŘ. Refinement of Trypanosoma U6 RNA Stem-Loop Structure Using 1- and 2-Bond Residual Dipolar Couplings. In 19th NMR Valtice. Brno: Masarykova universita v Brně, 2004, s. 30-30. ISBN 80-210-3352-5.

@inproceedings{571832,
   author = {Novák, Petr and Žídek, Lukáš and Padrta, Petr and Sklenář, Vladimír},
   address = {Brno},
   booktitle = {19th NMR Valtice},
   keywords = {NMR; Residual dipolar couplings; RNA; structure;},
   language = {eng},
   location = {Brno},
   isbn = {80-210-3352-5},
   pages = {30-30},
   publisher = {Masarykova universita v Brně},
   title = {Refinement of Trypanosoma U6 RNA Stem-Loop Structure Using 1- and 2-Bond Residual Dipolar Couplings},
   year = {2004}
}

TY  - CONF
ID  - 571832
AU  - Novák, Petr - Žídek, Lukáš - Padrta, Petr - Sklenář, Vladimír
PY  - 2004
TI  - Refinement of Trypanosoma U6 RNA Stem-Loop Structure Using 1- and 2-Bond Residual Dipolar Couplings
PB  - Masarykova universita v Brně
CY  - Brno
SN  - 8021033525
KW  - NMR
KW  - Residual dipolar couplings
KW  - RNA
KW  - structure;
N2  - The spliceosome is a complex of proteins and small nuclear RNAs (snRNAs) responsible for the removal of introns from pre-mRNA in eukaryotes. U6 snRNA has been demonstrated to be a vital element in splicing and its sequence is the most conserved of all snRNAs[1]. U6 snRNA from trypanosoma was our study. Determination of the solution structure of nucleic acid fragments is significantly improved if residual dipolar are measured. This data provides us additional long-range and local geometry restraints using a small degree of molecular alignment with the static magnetic field. We wanted to test polyethylenglycol (PEG) as a alignment medium for measuring the residual dipolar couplings (RDCs). PEG is a stable and unexpensive organic polymer that does not require a complex biochemical preparation like the most often used bacteriophage Pf1. In our study, applicability of PEG to several spin-state-selective E.COSY experiments was investigated. Measured RDCs were tested for internal consistency as refered in [2] in order to check the reliability of the values obtained in the PEG-aligned samples.
ER  -

NOVÁK, Petr; Lukáš ŽÍDEK; Petr PADRTA a Vladimír SKLENÁŘ. Refinement of Trypanosoma U6 RNA Stem-Loop Structure Using 1- and 2-Bond Residual Dipolar Couplings. In \textit{19th NMR Valtice}. Brno: Masarykova universita v Brně, 2004, s.~30-30. ISBN~80-210-3352-5.

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