In vitro testing of vancomycin - gentamicin loaded bone cement
to prevent prosthetic joint infection

J 2005

In vitro testing of vancomycin - gentamicin loaded bone cement to prevent prosthetic joint infection

GALLO, Jiří; Milan KOLÁŘ; Anthony V. FLORSCHÜTZ; Radek NOVOTNÝ; Roman PANTŮČEK et al.

Základní údaje

Originální název

In vitro testing of vancomycin - gentamicin loaded bone cement to prevent prosthetic joint infection

Název česky

Testování kostního cementu plněného vankomycinem-gentamicinem pro prevenci kloubních protetických infekcí

Autoři

GALLO, Jiří; Milan KOLÁŘ; Anthony V. FLORSCHÜTZ; Radek NOVOTNÝ; Roman PANTŮČEK a Michaela KESSELOVÁ

Vydání

Biomedical Papers, Olomouc, Palacký University, 2005, 1213-8118

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

[Full Text], URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/05:00013848

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Prosthetic joint infection; Polymethylmethacrylate; Antibiotic loaded cement; Vancomycin;Gentamicin; Staphylococcus aureus; Staphylococcus epidermidis

Štítky

Antibiotic loaded cement, Gentamicin, Polymethylmethacrylate, prosthetic joint infection, Staphylococcus aureus, Staphylococcus epidermidis, vancomycin

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 15. 2. 2008 09:37, prof. RNDr. Roman Pantůček, Ph.D.

Anotace

ORIG CZ

V originále

Sepsis is a greatly feared complication of total joint arthroplasty. One key question is how to prevent periopera-tive bacterial adherence, and therefore the potential for infectious complications. The objective of our study was to appraise the emerging capacity of staphylococcal survival on prosthetic materials and to analyze the in vitro eects of gentamicin and vancomycin loaded polymethylmethacrylate (PMMA) cement on bacterial adherence and growth. Hospital acquired staphylococcal strains were systematically inoculated on four orthopedic materials (ultrahigh mo-lecular weight polyethylene, PMMA without antibiotic, commercially produced PMMA loaded with gentamicin, and manually mixed PMMA loaded with gentamicin and vancomycin). Staphylococci were identied using culture and biochemical tests. The inoculated material was allowed to incubate in a liquid broth growth media and subsequently prepared for scanning electron microscopy and bacterial growth quantication. Materials without antibiotics showed evidence of staphylococcal growth. PMMA loaded with only gentamicin grew methicillin-resistant Staphylococcus aureus. Gentamicin-vancomycin loaded PMMA completely inhibited any bacterial growth.Low-dose gentamicin-vancomycin loaded PMMA prevents staphylococcal colonization better than commercially manufactured PMMA loaded with gentamicin. We recommend this combination in high-risk procedures and revision surgeries requiring bone cement.

Česky

neuvedeno

Návaznosti

MSM0021622415, záměr

Název: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Molekulární podstata buněčných a tkáňových regulací

Citovat

GALLO, Jiří; Milan KOLÁŘ; Anthony V. FLORSCHÜTZ; Radek NOVOTNÝ; Roman PANTŮČEK a Michaela KESSELOVÁ. In vitro testing of vancomycin - gentamicin loaded bone cement to prevent prosthetic joint infection. Biomedical Papers. Olomouc: Palacký University, 2005, roč. 149, č. 1, s. 153-158. ISSN 1213-8118.

@article{573853,
   author = {Gallo, Jiří and Kolář, Milan and Florschütz, Anthony V. and Novotný, Radek and Pantůček, Roman and Kesselová, Michaela},
   article_location = {Olomouc},
   article_number = {1},
   keywords = {Prosthetic joint infection; Polymethylmethacrylate; Antibiotic loaded cement; Vancomycin;Gentamicin; Staphylococcus aureus; Staphylococcus epidermidis},
   language = {eng},
   issn = {1213-8118},
   journal = {Biomedical Papers},
   title = {In vitro testing of vancomycin - gentamicin loaded bone cement to prevent prosthetic joint infection},
   url = {http://publib.upol.cz/~obd/fulltext/Biomed/2005/1/153.pdf},
   volume = {149},
   year = {2005}
}

TY  - JOUR
ID  - 573853
AU  - Gallo, Jiří - Kolář, Milan - Florschütz, Anthony V. - Novotný, Radek - Pantůček, Roman - Kesselová, Michaela
PY  - 2005
TI  - In vitro testing of vancomycin - gentamicin loaded bone cement to prevent prosthetic joint infection
JF  - Biomedical Papers
VL  - 149
IS  - 1
SP  - 153-158
EP  - 153-158
PB  - Palacký University
SN  - 12138118
KW  - Prosthetic joint infection
KW  - Polymethylmethacrylate
KW  - Antibiotic loaded cement
KW  - Vancomycin;Gentamicin
KW  - Staphylococcus aureus
KW  - Staphylococcus epidermidis
UR  - http://publib.upol.cz/~obd/fulltext/Biomed/2005/1/153.pdf
N2  - Sepsis is a greatly feared complication of total joint arthroplasty. One key question is how to prevent periopera-tive bacterial adherence, and therefore the potential for infectious complications. The objective of our study was to appraise the emerging capacity of staphylococcal survival on prosthetic materials and to analyze the in vitro eects of gentamicin and vancomycin loaded polymethylmethacrylate (PMMA) cement on bacterial adherence and growth. Hospital acquired staphylococcal strains were systematically inoculated on four orthopedic materials (ultrahigh mo-lecular weight polyethylene, PMMA without antibiotic, commercially produced PMMA loaded with gentamicin, and manually mixed PMMA loaded with gentamicin and vancomycin). Staphylococci were identied using culture and biochemical tests. The inoculated material was allowed to incubate in a liquid broth growth media and subsequently prepared for scanning electron microscopy and bacterial growth quantication. Materials without antibiotics showed evidence of staphylococcal growth. PMMA loaded with only gentamicin grew methicillin-resistant Staphylococcus aureus. Gentamicin-vancomycin loaded PMMA completely inhibited any bacterial growth.Low-dose gentamicin-vancomycin loaded PMMA prevents staphylococcal colonization better than commercially manufactured PMMA loaded with gentamicin. We recommend this combination in high-risk procedures and revision surgeries requiring bone cement.
ER  -

GALLO, Jiří; Milan KOLÁŘ; Anthony V. FLORSCHÜTZ; Radek NOVOTNÝ; Roman PANTŮČEK a Michaela KESSELOVÁ. In vitro testing of vancomycin - gentamicin loaded bone cement to prevent prosthetic joint infection. \textit{Biomedical Papers}. Olomouc: Palacký University, 2005, roč.~149, č.~1, s.~153-158. ISSN~1213-8118.

Přehled o publikaci