2005
Intra- and extraneuronal changes of immunofluorescence staining for inflammation-related molecules in the experimental models of peripheral neuropathic pain
DUBOVÝ, Petr; Radim JANČÁLEK; Ilona KLUSÁKOVÁ; Ivana SVÍŽENSKÁ; Kateřina PEJCHALOVÁ et al.Základní údaje
Originální název
Intra- and extraneuronal changes of immunofluorescence staining for inflammation-related molecules in the experimental models of peripheral neuropathic pain
Název česky
Intra- a extraneuronální změny v imunofluorescenční lokalizaci prozánětlivých a protizánětlivých molekul v experimentálních modelech neuropatické bolesti
Autoři
Vydání
Košice, Folia Medica Cassoviensia, od s. 22-22, 1 s. 2005
Nakladatel
Institute of Neurobiology, Slovak Academy of Science
Další údaje
Jazyk
angličtina
Typ výsledku
Stať ve sborníku
Obor
30000 3. Medical and Health Sciences
Stát vydavatele
Slovensko
Utajení
není předmětem státního či obchodního tajemství
Označené pro přenos do RIV
Ano
Kód RIV
RIV/00216224:14110/05:00012984
Organizační jednotka
Lékařská fakulta
ISBN
80-7097-607-1
Klíčová slova anglicky
dorsal root ganglia; TNF-alpha; TNFR1
Štítky
Změněno: 26. 6. 2009 10:39, prof. RNDr. Petr Dubový, CSc.
V originále
Peripheral neuropathic pain has been investigated using rat peripheral nerve injury models including unilateral sciatic nerve transection or ligation, spinal nerve ligation or ventral root transection. The experimental models were utilized for the study of immunofluorescence (IF) staining for IL-1b, IL-6, TNFa and TNFaR1 in the rat DRG. Quantitative and qualitative IF changes in the DRG affected by unilateral nerve injury were compared with the contralateral ones removed from operated animals as well as naive rats. Ipsi- and contralateral DRG displayed increased IF for TNFa and TNFaR1 when compared with DRG of naive rats. The TNFa IF was increased in both DRG neurons and their satellite glial cells, while enhanced IF for TNFaR1 was observed only at the surface of satellite glial cells after various types of nerve injury. In addition, ipsilateral DRG exhibited an elevation of TNFa immunoreactivity by invaded ED-1+ macrophages. The IL-1b IF was increased in the DRG neurons following sciatic nerve constriction, but not after the nerve trasection. A higher level of IF for IL-6 was observed in the DRG neurons and their satellite glial cells following sciatic nerve ligature as well as ventral root transection.
Česky
Intra- a extraneuronální změny v imunofluorescenční lokalizaci prozánětlivých a protizánětlivých molekul v experimentálních modelech neuropatické bolesti
Návaznosti
| GA309/03/1199, projekt VaV |
| ||
| MSM0021622404, záměr |
|