2005
Surface immobilized protein multilayers for cell seeding
DVOŘÁK, Petr; E. BRYNDA; Jiří PACHERNÍK; M. HOUSKA; Z. PIENTKA et al.Základní údaje
Originální název
Surface immobilized protein multilayers for cell seeding
Autoři
DVOŘÁK, Petr; E. BRYNDA; Jiří PACHERNÍK; M. HOUSKA a Z. PIENTKA
Vydání
Langmuir, 2005, 0743-7463
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
Genetika a molekulární biologie
Stát vydavatele
Spojené státy
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 3.705
Označené pro přenos do RIV
Ano
Kód RIV
RIV/00216224:14110/05:00028930
Organizační jednotka
Lékařská fakulta
UT WoS
Klíčová slova anglicky
MOUSE EMBRYOS; BIOSENSORS; ALBUMIN; HEPARIN; INVITRO; PLASMA
Štítky
Změněno: 15. 3. 2010 13:26, prof. Ing. Petr Dvořák, CSc.
Anotace
V originále
The adhesion and growth of mouse embryonic stem cells line D3 were tested in polystyrene culture dishes coated with the multilayer assemblies containing various cell-adhesive proteins such as gelatin, collagen IV, and laminin or polycations polylysine and poly(ethyleneimine). The cells were seeded in complete serum-containing media or in serum-free media and in the presence (non-differentiated) and/or absence (differentiated) of leukemia inhibitory factor. The cells grew best on surfaces coated with gelatin and collagen IV assemblies. There were no significant differences in the growth of the non-differentiated and differentiated cells in complete serum-containing media. When seeded in serum-free media, non-differentiated cells grew better than the differentiated ones. The layer-by-layer deposition appears to be a practicable technique by which scaffolds for tissue engineering can be coated with biomolecular assemblies tailored to specific cells and applications.
Návaznosti
| GA102/03/0633, projekt VaV |
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| GA203/02/1326, projekt VaV |
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| LN00A065, projekt VaV |
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