Humic Acids as Potential Activators of the Aryl Hydrocarbon
Receptor

D 2004

Humic Acids as Potential Activators of the Aryl Hydrocarbon Receptor

BITTNER, Michal; Jaroslav JANOŠEK; Luděk BLÁHA; Klára HILSCHEROVÁ; Ivan HOLOUBEK et al.

Základní údaje

Originální název

Humic Acids as Potential Activators of the Aryl Hydrocarbon Receptor

Název česky

Huminové kyseliny jako možné aktivátory AhR

Autoři

BITTNER, Michal; Jaroslav JANOŠEK; Luděk BLÁHA; Klára HILSCHEROVÁ; Ivan HOLOUBEK a John Paul GIESY

Vydání

1. vyd. USA, First Biennial Central & Eastern European Environmental Health Conference, od s. 22-23, 2 s. 2004

Nakladatel

Texas A&M University

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

30304 Public and environmental health

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/04:00019957

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Humic acids; AhR

Štítky

AhR, Humic acids

Změněno: 1. 2. 2006 08:46, RNDr. Mgr. Michal Bittner, Ph.D.

Anotace

ORIG CZ

V originále

Pure humic substances (HS) are generally regarded to be inert in ecosystems. Ecotoxicological function of HS in environment is often discussed in connection with the sorption of heavy metals and various organic xenobiotics, consequently decreasing their bioavailability and toxicity. But there were published some studies that HS can probably induce direct adverse effect on human health. That is discussed with connection to endemic appearance of Black foot disease and Kashin-Becks disease in some areas in China and Taiwan. Some experiments showed that oxidative stress caused by HS leads to damage of erythrocytes and fragmentation of DNA with subsequent apoptosis in chondrocytes. HS are the major components of the mixture of materials that comprise soil organic matter, and represent the major components of dissolved organic matter in waters. Chemical constitution of HS depends on the source of degraded organic materials, consequently HS comprise a variety of molecular structures and it is not possible to determine their unified molecular structure. Core of HS consists mostly of alkylaromatic, aliphatic and quinoide structures, and periphery from amino- and carboxyl acids and carbonyl-, phenyl- and hydroxyl groups. Aryl hydrocarbon receptor (AhR) is inducible ligand dependent transcription factor that mediates the biological and toxic effects of a structurally diverse range of chemicals, such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (i.e. the most potent activator of AhR), but also tetrapyroles, flavonoides and indole-containing substances. Dioxin-like toxicity of HS was evaluated using reporter gene assay with stably transfected H4IIE.luc hepatoma cells with a luciferase reporter gene under the control of dioxin-responsive element. We tested three different kinds of humic acids that were isolated from peat, soil and coal. Significant potencies to induce AhR-mediated effects of all tested humic acids were observed. Our preliminary results suggest that HS could activate AhR and cause subsequent effects. The research was supported by the Grant Agency of the Czech Republic, No. 525/03/0367.

Česky

Přeložte si anglický.

Návaznosti

GA525/03/0367, projekt VaV

Název: Ekotoxikologie persistentních organických polutantů životního prostředí

Investor: Grantová agentura ČR, Ekotoxikologie persistentních organických polutantů životního prostředí

Citovat

BITTNER, Michal; Jaroslav JANOŠEK; Luděk BLÁHA; Klára HILSCHEROVÁ; Ivan HOLOUBEK a John Paul GIESY. Humic Acids as Potential Activators of the Aryl Hydrocarbon Receptor. In First Biennial Central & Eastern European Environmental Health Conference. 1. vyd. USA: Texas A&M University, 2004, s. 22-23.

@inproceedings{618482,
   author = {Bittner, Michal and Janošek, Jaroslav and Bláha, Luděk and Hilscherová, Klára and Holoubek, Ivan and Giesy, John Paul},
   address = {USA},
   booktitle = {First Biennial Central & Eastern European Environmental Health Conference},
   edition = {1.},
   keywords = {Humic acids; AhR},
   language = {eng},
   location = {USA},
   pages = {22-23},
   publisher = {Texas A&M University},
   title = {Humic Acids as Potential Activators of the Aryl Hydrocarbon Receptor},
   year = {2004}
}

TY  - CONF
ID  - 618482
AU  - Bittner, Michal - Janošek, Jaroslav - Bláha, Luděk - Hilscherová, Klára - Holoubek, Ivan - Giesy, John Paul
PY  - 2004
TI  - Humic Acids as Potential Activators of the Aryl Hydrocarbon Receptor
PB  - Texas A&M University
CY  - USA
KW  - Humic acids
KW  - AhR
N2  - Pure humic substances (HS) are generally regarded to be inert in ecosystems. Ecotoxicological function of HS in environment is often discussed in connection with the sorption of heavy metals and various organic xenobiotics, consequently decreasing their bioavailability and toxicity. But there were published some studies that HS can probably induce direct adverse effect on human health. That is discussed with connection to endemic appearance of Black foot disease and Kashin-Becks disease in some areas in China and Taiwan. Some experiments showed that oxidative stress caused by HS leads to damage of erythrocytes and fragmentation of DNA with subsequent apoptosis in chondrocytes. HS are the major components of the mixture of materials that comprise soil organic matter, and represent the major components of dissolved organic matter in waters. Chemical constitution of HS depends on the source of degraded organic materials, consequently HS comprise a variety of molecular structures and it is not possible to determine their unified molecular structure. Core of HS consists mostly of alkylaromatic, aliphatic and quinoide structures, and periphery from amino- and carboxyl acids and carbonyl-, phenyl- and hydroxyl groups. Aryl hydrocarbon receptor (AhR) is inducible ligand dependent transcription factor that mediates the biological and toxic effects of a structurally diverse range of chemicals, such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (i.e. the most potent activator of AhR), but also tetrapyroles, flavonoides and indole-containing substances. Dioxin-like toxicity of HS was evaluated using reporter gene assay with stably transfected H4IIE.luc hepatoma cells with a luciferase reporter gene under the control of dioxin-responsive element. We tested three different kinds of humic acids that were isolated from peat, soil and coal. Significant potencies to induce AhR-mediated effects of all tested humic acids were observed. Our preliminary results suggest that HS could activate AhR and cause subsequent effects. The research was supported by the Grant Agency of the Czech Republic, No. 525/03/0367.
ER  -

BITTNER, Michal; Jaroslav JANOŠEK; Luděk BLÁHA; Klára HILSCHEROVÁ; Ivan HOLOUBEK a John Paul GIESY. Humic Acids as Potential Activators of the Aryl Hydrocarbon Receptor. In \textit{First Biennial Central \&{} Eastern European Environmental Health Conference}. 1. vyd. USA: Texas A\&{}M University, 2004, s.~22-23.

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