2005
Activation of Ah-receptor by humic substances
BITTNER, Michal, Jaroslav JANOŠEK, Luděk BLÁHA, Klára HILSCHEROVÁ, Ivan HOLOUBEK et. al.Základní údaje
Originální název
Activation of Ah-receptor by humic substances
Název česky
Aktivace AhR huminovými látkami
Autoři
BITTNER, Michal (203 Česká republika, garant), Jaroslav JANOŠEK (203 Česká republika), Luděk BLÁHA (203 Česká republika), Klára HILSCHEROVÁ (203 Česká republika), Ivan HOLOUBEK (203 Česká republika) a John Paul GIESY (840 Spojené státy)
Vydání
2005. vyd. Brno, Advances and Trends in Ecotoxicology, od s. 115-115, 1 s. 2005
Nakladatel
MU
Další údaje
Jazyk
angličtina
Typ výsledku
Stať ve sborníku
Obor
30304 Public and environmental health
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Kód RIV
RIV/00216224:14310/05:00014613
Organizační jednotka
Přírodovědecká fakulta
ISBN
80-210-3799-7
Klíčová slova anglicky
Humic acid
Štítky
Změněno: 25. 6. 2007 21:23, doc. Mgr. Klára Hilscherová, Ph.D.
V originále
Humic substances (HS) are ubiquitous in the environment. However, some studies indicate that HS can induce a direct adverse effect on human health and hormone-like effects on fish, amphibians and invertebrates. In the present study we investigated possible biochemical mechanism of HS toxicity using reporter gene in vitro bioassay with H4IIE.luc cells. H4IIE.luc are stably transfected hepatoma cells with a luciferase reporter gene that is activated via cytosolic aryl hydrocarbon receptor (AhR). The most potent activator of AhR is 2,3,7,8-tetrachlorodibenzo-p-dioxin, but number of structurally diverse compounds has also been found to activate AhR. Aim of this study was to evaluate the dioxin-like activity of humic substances standards purchased from IHSS and Sigma-Aldrich companies. HS were dissolved in 0.05M NaOH, subsequently, lipophilic compounds (including fragments of HS) were extracted by liquid-liquid extraction with hexane/dichloromethane. Organic extract of HS was further treated with sulphuric acid to study the role of possible trace persistent contaminants (e.g. PCDDs, PCBs...). Dioxin-like activities of alkali solutions, organic extract and acid treated extracts have been evaluated using H4IIE.luc cells. Levels of possible known AhR-active contaminants were determined by GC-MS. Our results showed that both aqueous solutions and organic extracts of five out of twelve tested HS were active in luciferase assay. These results clearly indicated that some HS (or their fragments) might elicit significant inductions of AhR mediated effects. This might possibly lead to consequent health hazards.
Česky
Přeložte si anglický.
Návaznosti
| GP525/05/P160, projekt VaV |
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| MSM0021622412, záměr |
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