SLUNSKÁ, Zdenka, Iva SLANINOVÁ, Jiri SINKORA, Marcela VLKOVA and Eva TÁBORSKÁ. Biological effects of benzo[c]phenanthridine alkaloids. In Journal of Applied Biomedicine. Volume 3. České Budějovice, CR: University of South Bohemia, Czech Repub, 2005, p. 42-43. ISBN 1214-021X.
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Basic information
Original name Biological effects of benzo[c]phenanthridine alkaloids.
Name in Czech Biologické aktivity benzo[c]phenanthridinových alkaloidů
Authors SLUNSKÁ, Zdenka (203 Czech Republic), Iva SLANINOVÁ (203 Czech Republic, guarantor), Jiri SINKORA (203 Czech Republic), Marcela VLKOVA (203 Czech Republic) and Eva TÁBORSKÁ (203 Czech Republic).
Edition Volume 3. České Budějovice, CR, Journal of Applied Biomedicine, p. 42-43, 2 pp. 2005.
Publisher University of South Bohemia, Czech Repub
Other information
Original language English
Type of outcome Proceedings paper
Field of Study Genetics and molecular biology
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14110/05:00013264
Organization unit Faculty of Medicine
ISBN 1214-021X
Keywords in English benzo[c]phenanthridine alkaloids; apoptosis; cytotoxicity; MTT assay
Tags apoptosis, Benzo[c]phenanthridine Alkaloids, cytotoxicity, MTT assay
Changed by Changed by: prof. MUDr. Iva Slaninová, Ph.D., učo 2105. Changed: 19/1/2007 09:53.
Abstract
Quaternary benzo[c]phenanthridine alkaloids (QBA) are group of natural products, which are discussed as potencial cytostatics for cancer treatment. QBA are relatively small group of isoquinoline alkaloids that have phenylalanin as their precursor. They are distributed in a number of plant species of Papaveraceae, Fumariaceae and Rutaceae families. Sanguinarin and chelerythrin are the two best known QBA and their biological activities have been widely studied (1,2,3). In addition to them about 10 other QBA differing only by substitution on the aromatic rings have been isolated as a minor components. The informations about biological effects of this minor QBA are rare. In this study the differential cytotoxicity of sanguinarine (SA), chelerythrine (CHE) and their minority derivates sanguirubine (SR), chelirubine (CHR) and macarpine (MA) was assessed in vitro by MTT assay. One normal (skin fibroblasts) and 3 tumour cell lines (HeLa; A 431; HL-60) were used as a model object. After 72 hours, MTT assay was performed to evaluate the in vitro cytotoxicity induced by tested alkaloids. Our results show that HL-60 and skin fibroblasts were the most sensitive cell culture lines, while the carcinoma cell lines HeLa and A-431 appeared much more resistant. For apoptosis detection morphology of nuclei of alkaloid treated HL-60 cells after DAPI staining was studied and flow cytometric analysis using Annexin V-FITC and PI was applied. All tested alkaloids appeared to be inductors of apoptosis. Based on our observations we concluded that alkaloids tested in this study show strong antiproliferative and proapoptotic effect in vitro. This work was supported by the grants 525/040017 and 301/03/H005 from the Grant agency of Czech republic and MSM0021622415 from Ministry of Education of the Czech Republic References: 1) Walterová, D., Ulrychová, J., Válka, I., et al., Acta Univ. Palacki Olomouc Fac. Med. 1995; 139:7-14. 2) Šimánek, V., Vespalec, R., Šedo,A., Ulrichová, J., Vičar, J. 2003. Quaternary Benzo(c)phenanthridine alkaloids - biological activities. In: M.P.Schneider (Ed.), Chemical Probes in Biology. Kluwer Academic Publishers, Netherlands. pp 245-254. 3) Slaninová,I., E.Taborská, H.Bochořáková, and J.Slanina. 2001. Interaction of benzo[c]phenanthridine and protoberberine alkaloids with animal and yeast cells. Cell Biology and Toxicology 17:51-63.
Abstract (in Czech)
Qvarterní benzo[c]phenanthridinové alkaloidy (QBA) jsou skupinou přírodních produktů, které jsou v současnosti diskutovány jako potenciální cytostatika. Vyskytují se v rostlinách čeledí Papaveraceae, Fumariaceae a Rutaceae. Nejznámější a nejlépe prostudované jsou Sanguinarin and chelerytrin, informace o biologických aktivitách minoritních derivátů jsou vzácné. Tato studie se zabývá srovnáním antiproliferační aktivity sanguinarinu (SA), chelerythrinu (CHE) a minoritních derivátů sanguirubine (SR), chelirubine (CHR) and macarpinu (MA) na linii normálních lidských kožních fibroblastů a 3 nádorové buněčné linie (HeLa; A 431; HL-60). Antiproliferační aktivita byla stanovena po 72 hodinové kultivaci pomocí MTT assae, schopnost indukovat apoptózu byla studována pomocí kombinace Anexinu V-FITC a propidium jodidu a hodnocena kvantitastivne průtokovou cytometrií.
Links
GA525/04/0017, research and development projectName: Rostlinné zdroje benzofenanthridinových alkaloidů a účinek těchto alkaloidů na normální a nádorové buňky
Investor: Czech Science Foundation, Plant sources of benzophenanthridine alkaloids and effects of these alkaloid on normal and cancer cells
GD301/03/H005, research and development projectName: Molekulární a buněčná biologie v biomedicíně
Investor: Czech Science Foundation, Molecular and cell biology in biomedicine
MSM0021622415, plan (intention)Name: Molekulární podstata buněčných a tkáňových regulací
Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations
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