2005
Effect of procathepsin D activation peptide on gene expression of breast cancer cells
BENEŠ, Petr; A. VASHISHTA; S. SARASWAT-OHRI; M. FUSEK; Šárka POSPÍŠILOVÁ et al.Basic information
Original name
Effect of procathepsin D activation peptide on gene expression of breast cancer cells
Name in Czech
Efekt aktivačního peptidu procathepsinu D na genovou expresi buněk karcinomu prsu
Authors
BENEŠ, Petr; A. VASHISHTA; S. SARASWAT-OHRI; M. FUSEK; Šárka POSPÍŠILOVÁ; Boris TICHÝ ORCID and V. VĚTVIČKA
Edition
Cancer Lett, 2005, 0304-3835
Other information
Language
English
Type of outcome
Article in a journal
Field of Study
30200 3.2 Clinical medicine
Country of publisher
Czech Republic
Confidentiality degree
is not subject to a state or trade secret
Impact factor
Impact factor: 3.049
Marked to be transferred to RIV
No
Organization unit
Faculty of Medicine
UT WoS
000239553500006
Keywords in English
breast cancer; gene expression
Tags
Changed: 6/4/2010 10:00, MVDr. Boris Tichý, Ph.D.
In the original language
Overexpression of procathepsin D (pCD) is reported to occur in numerous types of cancer and is associated with increased growth and metastasis. It has been established that pCD affects multiple stages of tumor progression including proliferation, angiogenesis and metastasis. Previously, we showed that the mitogenic effect of pCD on cancer cells is mediated by interaction of its activation peptide (AP) with yet unidentified cell surface receptor. In this investigation, gene expression profiles were compared between AP-treated and control human breast cancer ZR-75-1 cells to elucidate the mechanism of AP mitogenicity. Several differentially expressed genes involved in signal transduction, regulation of cell cycle, apoptosis, tumor invasion and metastasis were identified using microarray technology. These findings, including overexpression of NF-kappaB2, were confirmed in breast cancer cell lines by reverse-transcriptase PCR (RT-PCR). Understanding the mechanism of pCDs effect on breast cancer cells could extend possibilities of breast cancer treatment in the future.
In Czech
Zvýšená exprese procathepsinu D (pCD) byla popsána u mnoha typů nádorových buněk a byla asociována s vyšší růstovou aktivitou a zvýšenou schopnosí metastazovat. Bylo prokázáno, že pCD ovlivňuje procesy jako je proliferace, angiogeneze a metastazování. Dříve jsme prokázali, že mitogenní efekt pCD je způsoben interakcí jeho aktivačního peptidu (AP) s dosud neznámým povrchovým receptorem. Tyto objevy, zahrnující i zvýšenou expresi NF-kappaB2, byly potvrzeny pomocí RT-PCR. Pochopení efektu pCD u buněk karcinomu prsu může v budoucnosti vést k rozšíření možností protinádorové terapie.
Links
| NR8448, research and development project |
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