| ZEISBERGEROVÁ, Marta, Vratislav KOŠŤÁL, Markéta ŠRÁMKOVÁ, Pavel BABICA, Luděk BLÁHA, Zdeněk GLATZ a Vladislav KAHLE. Separation of microcystins by capillary electrochromatography in monolithic columns. Journal of Chromatography B. Amsterdam, The Netherlands: Elsevier, 2006, roč. 841, 1-2, s. 140-144. ISSN 1570-0232. |
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@article{636179,
author = {Zeisbergerová, Marta and Košťál, Vratislav and Šrámková, Markéta and Babica, Pavel and Bláha, Luděk and Glatz, Zdeněk and Kahle, Vladislav},
article_location = {Amsterdam, The Netherlands},
article_number = {1-2},
keywords = {microcystines; capillary electrochromatography; monolithic columns},
language = {eng},
issn = {1570-0232},
journal = {Journal of Chromatography B},
title = {Separation of microcystins by capillary electrochromatography in monolithic columns},
volume = {841},
year = {2006}
}
TY - JOUR
ID - 636179
AU - Zeisbergerová, Marta - Košťál, Vratislav - Šrámková, Markéta - Babica, Pavel - Bláha, Luděk - Glatz, Zdeněk - Kahle, Vladislav
PY - 2006
TI - Separation of microcystins by capillary electrochromatography in monolithic columns
JF - Journal of Chromatography B
VL - 841
IS - 1-2
SP - 140-144
EP - 140-144
PB - Elsevier
SN - 15700232
KW - microcystines
KW - capillary electrochromatography
KW - monolithic columns
N2 - Contribution on microcystin variant analysis by capillary electrochromatography (CEC) with easily affordable spectrophotometric detection is presented. Two types of reversed-phase capillary columns formed by inorganic or organic polymer monoliths were prepared for this purpose. The analyses were performed isocratically by means of tris(hydroxymethyl) aminomethane (TRIS) buffers of mildly alkaline pH containing 30% (v/v) acetonitrile as the mobile phases. The samples were injected electrokinetically and the analyses were done at the same separation field strength of 500 V/cm. Microcystins were detected at 238 nm. Although both column types differ not only in monolith quality (inorganic versus organic) but also in the length of the aliphatic moiety (C8 versus C12) similar results were achieved. The on-column preconcentration as the encouraging prospect of electrochromatographic technique was also tested. Consequently 5% of column volume was injected in contrast with 0.5% at standard injection scheme resulting in the six times enrichment of the low concentrated cyanobacterial extract at the top of the separation column. From these preliminary results can be seen that the CEC method is fully applicable for rapid microcystins screening.
ER -
ZEISBERGEROVÁ, Marta, Vratislav KOŠŤÁL, Markéta ŠRÁMKOVÁ, Pavel BABICA, Luděk BLÁHA, Zdeněk GLATZ a Vladislav KAHLE. Separation of microcystins by capillary electrochromatography in monolithic columns. \textit{Journal of Chromatography B}. Amsterdam, The Netherlands: Elsevier, 2006, roč.~841, 1-2, s.~140-144. ISSN~1570-0232.
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