2006
Increased plasma levels of sRAGE in patients with diabetic nephropathy
KAŇKOVÁ, Kateřina, Marta KALOUSOVÁ, Miluše HERTLOVÁ, Darja KRUSOVÁ, Tomáš ZIMA et. al.Základní údaje
Originální název
Increased plasma levels of sRAGE in patients with diabetic nephropathy
Název česky
Zvýšené plazmatické hladiny sRAGE u pacientů s diabetickou nefropatií
Autoři
KAŇKOVÁ, Kateřina (203 Česká republika, garant), Marta KALOUSOVÁ (203 Česká republika), Miluše HERTLOVÁ (203 Česká republika), Darja KRUSOVÁ (203 Česká republika) a Tomáš ZIMA (203 Česká republika)
Vydání
2006
Další údaje
Jazyk
angličtina
Typ výsledku
Konferenční abstrakt
Obor
30202 Endocrinology and metabolism
Stát vydavatele
Německo
Utajení
není předmětem státního či obchodního tajemství
Kód RIV
RIV/00216224:14110/06:00015385
Organizační jednotka
Lékařská fakulta
UT WoS
000240626201517
Klíčová slova anglicky
diabetes mellitus; diabetic nephropathy; RAGE; AGEs; susceptibility
Štítky
Změněno: 2. 4. 2010 15:15, prof. MUDr. Kateřina Kaňková, Ph.D.
V originále
Background and Aims: Advanced Glycation End products (AGEs) accumulate in diabetic patients in a greater rate than in age-matched non-diabetic subjects and exert various adverse effects via the Receptor of AGE (RAGE). Soluble RAGE (sRAGE) is a 50kDa naturally occurring C-terminally truncated form of RAGE that functions as an inhibitor of AGE-mediated signalling events. Decreased levels of supposedly protective sRAGE were ascertained in patients with macrovascular disease and hypertension. Data about the role of sRAGE in diabetic nephropathy are so far lacking. The aim of the study was to analyse the relationship between sRAGE and renal functions in subjects with diabetic nephropathy and, hereby, implicit protective capacity against AGE-mediated toxicity. Material and Methods: Study was designed as a cross-sectional analysis of a cohort of 255 diabetic patients with variable extent of diabetic nephropathy. Total number of subjects consisted if 36 diabetics with T1DM, 219 with T2DM (approx. 1:1 male-to-female ratio). Mean duration of diabetes was 12.2 +/- 9.4 yrs, mean age was 61.3 +/- 14.5 yrs. Among all study subjects, 82 subjects had normoalbuminuria, 50 had persistent microalbuminuria, 112 had persistent proteinuria and 10 subjects had ESRD. Plasma concentration of sRAGE was assessed immunochemically (ELISA) using standard kits (Quantikine, RD Systems). Routine biochemical parameters were measured by certified methods using automated analysers. Relationship between circulating levels of sRAGE and the grade of diabetic nephropathy, renal function parameters and diabetes duration and compensation was analysed by means of non-parametric correlation, difference between groups were tested by Mann-Whitney test. Results: sRAGE was significantly higher in nephropathy subjects (any degree of DN) than normoalbuminuric patients (P=0.0015), 2154.4 +/- 2612 pg/ml vs. 1622.7 +/- 1981.4 pg/ml, respectively. In patients with nephropathy, sRAGE positively correlated with severity of nephropathy (P=0.0028). Further, sRAGE positively correlated with patient age, duration of diabetes, serum urea, serum creatinine and albumin excretion rate and negatively with glomerular filtration rate (all P<0.05). No significant correlation was ascertained with fasting plasma glucose or HbA1c. Conclusions: Contrary to our anticipations, serum sRAGE levels are in fact (i) increased in diabetics with diabetic nephropathy compared to normoalbuminuric subjects and (ii) further rise with decreasing renal function. It remains to be elucidated whether the increase merely reflects decreased renal clearance of sRAGE or up-regulation of sRAGE in order to protect cells against AGE-mediated effects.
Česky
Práce popisuje nález zvýšdených hladin sRAGE u osob s diabetickou nefropatií a identifikuje pokles GFR jako hlavníi faktor determinující jeho plazmatické koncentrace.
Návaznosti
| KJB501620601, projekt VaV |
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