The Smc5-Smc6 DNA Repair Complex: Bridging of the SMC5-SMC6
heads by the kleisin, Nse4 ...

J 2006

The Smc5-Smc6 DNA Repair Complex: Bridging of the SMC5-SMC6 heads by the kleisin, Nse4 ...

PALEČEK, Jan; Susanne VIDOT; Min FENG; Aidan DOHERTY; Alan LEHMANN et al.

Základní údaje

Originální název

The Smc5-Smc6 DNA Repair Complex: Bridging of the SMC5-SMC6 heads by the kleisin, Nse4 ...

Autoři

PALEČEK, Jan; Susanne VIDOT; Min FENG; Aidan DOHERTY a Alan LEHMANN

Vydání

Journal of Biological Chemistry, 2006

Další údaje

Typ výsledku

Článek v odborném periodiku

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

SMC complex, SMC5/6 architecture, kleisin subunit,

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 16. 12. 2010 15:44, doc. Mgr. Jan Paleček, Dr. rer. nat.

Anotace

V originále

Structural maintenance of chromosomes (SMC) proteins play fundamental roles in many aspects of chromosome organization and dynamics. The SMC complexes form unique structures with long coiled-coil arms folded at a hinge domain, so that the globular N- and C-terminal domains are brought together to form a "head." Within the Smc5-Smc6 complex, we previously identified two subcomplexes containing Smc6-Smc5-Nse2 and Nse1-Nse3-Nse4. A third subcomplex containing Nse5 and -6 has also been identified recently. We present evidence that Nse4 is the kleisin component of the complex, which bridges the heads of Smc5 and -6. The C-terminal part of Nse4 interacts with the head domain of Smc5, and structural predictions for Nse4 proteins suggest similar motifs that are shared within the kleisin family. Specific mutations within a predicted winged helix motif of Nse4 destroy the interaction with Smc5. We propose that Nse4 and its orthologs form the delta-kleisin subfamily. We further show that Nse3, as well as Nse5 and Nse6, also bridge the heads of Smc5 and -6. The Nse1-Nse3-Nse4 and Nse5-Nse6 subcomplexes bind to the Smc5-Smc6 heads domain at different sites.

Citovat

PALEČEK, Jan; Susanne VIDOT; Min FENG; Aidan DOHERTY a Alan LEHMANN. The Smc5-Smc6 DNA Repair Complex: Bridging of the SMC5-SMC6 heads by the kleisin, Nse4 ... Journal of Biological Chemistry. 2006, roč. 281, č. 48, s. 36952-9.

@article{700001,
   author = {Paleček, Jan and Vidot, Susanne and Feng, Min and Doherty, Aidan and Lehmann, Alan},
   article_number = {48},
   keywords = {SMC complex, SMC5/6 architecture, kleisin subunit,},
   journal = {Journal of Biological Chemistry},
   title = {The Smc5-Smc6 DNA Repair Complex: Bridging of the SMC5-SMC6 heads by the kleisin, Nse4 ...},
   url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=17005570&query_hl=1&itool=pubmed_docsum},
   volume = {281},
   year = {2006}
}

TY  - JOUR
ID  - 700001
AU  - Paleček, Jan - Vidot, Susanne - Feng, Min - Doherty, Aidan - Lehmann, Alan
PY  - 2006
TI  - The Smc5-Smc6 DNA Repair Complex: Bridging of the SMC5-SMC6 heads by the kleisin, Nse4 ...
JF  - Journal of Biological Chemistry
VL  - 281
IS  - 48
SP  - 36952-9
EP  - 36952-9
KW  - SMC complex, SMC5/6 architecture, kleisin subunit,
UR  - http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=17005570&query_hl=1&itool=pubmed_docsum
N2  - Structural maintenance of chromosomes (SMC) proteins play fundamental roles in many aspects of chromosome organization and dynamics. The SMC complexes form unique structures with long coiled-coil arms folded at a hinge domain, so that the globular N- and C-terminal domains are brought together to form a "head." Within the Smc5-Smc6 complex, we previously identified two subcomplexes containing Smc6-Smc5-Nse2 and Nse1-Nse3-Nse4. A third subcomplex containing Nse5 and -6 has also been identified recently. We present evidence that Nse4 is the kleisin component of the complex, which bridges the heads of Smc5 and -6. The C-terminal part of Nse4 interacts with the head domain of Smc5, and structural predictions for Nse4 proteins suggest similar motifs that are shared within the kleisin family. Specific mutations within a predicted winged helix motif of Nse4 destroy the interaction with Smc5. We propose that Nse4 and its orthologs form the delta-kleisin subfamily. We further show that Nse3, as well as Nse5 and Nse6, also bridge the heads of Smc5 and -6. The Nse1-Nse3-Nse4 and Nse5-Nse6 subcomplexes bind to the Smc5-Smc6 heads domain at different sites.
ER  -

PALEČEK, Jan; Susanne VIDOT; Min FENG; Aidan DOHERTY a Alan LEHMANN. The Smc5-Smc6 DNA Repair Complex: Bridging of the SMC5-SMC6 heads by the kleisin, Nse4 ... \textit{Journal of Biological Chemistry}. 2006, roč.~281, č.~48, s.~36952-9.

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