KUČERA, Igor. Interference of chlorate and chlorite with nitrate reduction in resting cells of Paracoccus denitrificans. Microbiology-SGM. Reading, Berks (Great Britain): Society for General Microbiology, 2006, roč. 152, č. 12, s. 3529-3534. ISSN 1350-0872. |
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@article{700507, author = {Kučera, Igor}, article_location = {Reading, Berks (Great Britain)}, article_number = {12}, keywords = {nitrate respiration; nitrate reductase; chlorate; chlorite; enzyme inactivation}, language = {eng}, issn = {1350-0872}, journal = {Microbiology-SGM}, title = {Interference of chlorate and chlorite with nitrate reduction in resting cells of Paracoccus denitrificans}, volume = {152}, year = {2006} }
TY - JOUR ID - 700507 AU - Kučera, Igor PY - 2006 TI - Interference of chlorate and chlorite with nitrate reduction in resting cells of Paracoccus denitrificans JF - Microbiology-SGM VL - 152 IS - 12 SP - 3529-3534 EP - 3529-3534 PB - Society for General Microbiology SN - 13500872 KW - nitrate respiration KW - nitrate reductase KW - chlorate KW - chlorite KW - enzyme inactivation N2 - When grown anaerobically on a succinate-nitrate medium (SN), Paracoccus denitrificans forms the membrane-bound, cytoplasmically-oriented, chlorate-reducing nitrate reductase Nar, while the periplasmic enzyme Nap is expressed during the aerobic growth on butyrate (BO). Preincubation of SN cells with chlorate produced a concentration-dependent decrease in nitrate utilization, which could be ascribed to Nar inactivation. Toluenisation rendered Nar less sensitive to chlorate, but more sensitive to chlorite. Nap enzyme of BO cells was inactivated by both chlorate and chlorite at concentrations that were at least two orders of magnitude lower than those shown to affect Nar. Partial purification of Nap resulted in an insensitivity to chlorate and a diminished sensitivity to chlorite. Azide was specific for SN cells in protecting nitrate reductase against chlorate attack, the protective effect of nitrate being more pronounced in BO cells. The results are discussed in terms of different metabolic activation of chlorine oxoanions in both types of cells and limited permeation of chlorite across the cell membrane. ER -
KUČERA, Igor. Interference of chlorate and chlorite with nitrate reduction in resting cells of Paracoccus denitrificans. \textit{Microbiology-SGM}. Reading, Berks (Great Britain): Society for General Microbiology, 2006, roč.~152, č.~12, s.~3529-3534. ISSN~1350-0872.
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