Minimization of adsorption in capillary zone electrophoresis of
proteins and neuroprotective peptides

D 2005

Minimization of adsorption in capillary zone electrophoresis of proteins and neuroprotective peptides

ŠULÁK, Ondřej; Miroslava SPANILÁ; Dagmar GAJDOŠOVÁ; Jiří PAZOUREK; Josef HAVEL et al.

Základní údaje

Originální název

Minimization of adsorption in capillary zone electrophoresis of proteins and neuroprotective peptides

Název česky

Minimalizace adsorpce proteinů a neuroprotektivních peptidů v kapilární zónové elektroforéze

Autoři

ŠULÁK, Ondřej; Miroslava SPANILÁ; Dagmar GAJDOŠOVÁ; Jiří PAZOUREK a Josef HAVEL

Vydání

Prague, Czech republic, Collection Symposium Series, Biologically Active Peptides, od s. 87-90, 4 s. 2005

Nakladatel

Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10406 Analytical chemistry

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/05:00020042

Organizační jednotka

Přírodovědecká fakulta

ISBN

8086241262

Klíčová slova anglicky

Capillary electrophoresis; Coating; Electroosmotic flow; Polyethyleneimine; 3glycidoxypropyltrimethoxysilane; 3aminopropyltriethoxysilane; Linear polyacrylamide

Štítky

3aminopropyltriethoxysilane, 3glycidoxypropyltrimethoxysilane, Capillary electrophoresis, coating, electroosmotic flow, Linear polyacrylamide, polyethyleneimine

Změněno: 16. 11. 2006 13:02, Mgr. Ondřej Šulák, Ph.D.

Anotace

ORIG CZ

V originále

Four types of coating procedures suitable for protein and peptides separation: covalent coating using polyethyleneimine (PEI), 3-glycidoxy-propyltrimethoxysilane (GPTMS), 3-aminopropyltriethoxysilane (APS) and a new approach of dynamic coating with linear polyacrylamide (LPA) have been studied. The capillary surface was characterized by determination of the electroosmotic flow (EOF); for optimization a model mixture of proteins (cytochrome C, lysozyme and ribonuclease A) was used. Separation of a neuroprotective peptide [Gly-14]humanin was demonstrated in the optimized system.

Česky

Návaznosti

GA305/03/1100, projekt VaV

Název: Syntéza a studium neuroprotektivních peptidů odvozených od humaninu

Citovat

ŠULÁK, Ondřej; Miroslava SPANILÁ; Dagmar GAJDOŠOVÁ; Jiří PAZOUREK a Josef HAVEL. Minimization of adsorption in capillary zone electrophoresis of proteins and neuroprotective peptides. In Collection Symposium Series, Biologically Active Peptides. Prague, Czech republic: Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, 2005, s. 87-90. ISBN 8086241262.

@inproceedings{701551,
   author = {Šulák, Ondřej and Spanilá, Miroslava and Gajdošová, Dagmar and Pazourek, Jiří and Havel, Josef},
   address = {Prague, Czech republic},
   booktitle = {Collection Symposium Series, Biologically Active Peptides},
   keywords = {Capillary electrophoresis; Coating; Electroosmotic flow; Polyethyleneimine; 3glycidoxypropyltrimethoxysilane; 3aminopropyltriethoxysilane; Linear polyacrylamide},
   language = {eng},
   location = {Prague, Czech republic},
   isbn = {8086241262},
   pages = {87-90},
   publisher = {Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic},
   title = {Minimization of adsorption in capillary zone electrophoresis of proteins and neuroprotective peptides},
   year = {2005}
}

TY  - CONF
ID  - 701551
AU  - Šulák, Ondřej - Spanilá, Miroslava - Gajdošová, Dagmar - Pazourek, Jiří - Havel, Josef
PY  - 2005
TI  - Minimization of adsorption in capillary zone electrophoresis of proteins and neuroprotective peptides
PB  - Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic
CY  - Prague, Czech republic
SN  - 8086241262
KW  - Capillary electrophoresis
KW  - Coating
KW  - Electroosmotic flow
KW  - Polyethyleneimine
KW  - 3glycidoxypropyltrimethoxysilane
KW  - 3aminopropyltriethoxysilane
KW  - Linear polyacrylamide
N2  - Four types of coating procedures suitable for protein and peptides separation: covalent coating using polyethyleneimine (PEI), 3-glycidoxy-propyltrimethoxysilane (GPTMS), 3-aminopropyltriethoxysilane (APS) and a new approach of dynamic coating with linear polyacrylamide (LPA) have been studied. The capillary surface was characterized by determination of the electroosmotic flow (EOF); for optimization a model mixture of proteins (cytochrome C, lysozyme and ribonuclease A) was used. Separation of a neuroprotective peptide [Gly-14]humanin was demonstrated in the optimized system.
ER  -

ŠULÁK, Ondřej; Miroslava SPANILÁ; Dagmar GAJDOŠOVÁ; Jiří PAZOUREK a Josef HAVEL. Minimization of adsorption in capillary zone electrophoresis of proteins and neuroprotective peptides. In \textit{Collection Symposium Series, Biologically Active Peptides}. Prague, Czech republic: Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, 2005, s.~87-90. ISBN~8086241262.

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