Isolation and Expansion of Allogeneic Myeloma-Specific
Interferon-Gamma Producing T Cells for Adoptive Immunotherapy

J 2006

Isolation and Expansion of Allogeneic Myeloma-Specific Interferon-Gamma Producing T Cells for Adoptive Immunotherapy

OČADLÍKOVÁ, Darina; Lenka ZAHRADOVÁ; Lucie KOVÁŘOVÁ; Miroslav PENKA; Jan ŽALOUDÍK et. al.

Základní údaje

Originální název

Isolation and Expansion of Allogeneic Myeloma-Specific Interferon-Gamma Producing T Cells for Adoptive Immunotherapy

Název česky

Izolace a expanze alogenních myelom-specifických a interferon gama-pozitivních T lymfocytů pro adoptivní imunoterapii

Autoři

OČADLÍKOVÁ, Darina; Lenka ZAHRADOVÁ; Lucie KOVÁŘOVÁ; Miroslav PENKA; Jan ŽALOUDÍK; Roman HÁJEK a Jaroslav MICHÁLEK

Vydání

Medical Oncology, Totowa, NJ, USA, Humana Press, 2006, 1559-131X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30200 3.2 Clinical medicine

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14110/06:00018909

Organizační jednotka

Lékařská fakulta

UT WoS

000242136000009

Klíčová slova anglicky

Multiple myeloma; immunotherapy; interferon-gamma; T cell;CFSE

Štítky

CFSE, Immunotherapy, interferon-gamma, multiple myeloma, T cell

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 22. 6. 2009 16:51, Mgr. Darina Očadlíková, Ph.D.

Anotace

ORIG CZ

V originále

Adoptive immunotherapy is a promising approach in the treatment of multiple myeloma. We have tested the identification, separation, and expansion of allogeneic myeloma-specific T cells in vitro. Irradiated myeloma cell line ARH 77 has been used to stimulate allogeneic CD4+ and CD8+ T lymphocytes. Activated myeloma-specific T cells that produced interferon-gamma were isolated using immunomagnetic beads and further expanded in vitro to numbers of up to 400 e06 T cells. Specificity of the T lymphocytes was tested using a 5-(6-)carboxyfluoresceine diacetate succinimidyl ester (CFSE)–based cytotoxicity test. This study demonstrates the feasibility of identification and isolation of tumor-specific T cells from allogeneic donors that can be expanded in vitro to numbers useful for clinical applications.

Česky

Izolace a expanze alogenních myelom-specifických interferon-gama produkujících T lymfocytů pro buněčnou imunoterapii u mnohočetného myelomu.

Návaznosti

NC7475, projekt VaV

Název: Hodnocení buněčné imunity a molekulární analýza myelom-specifických T lymfocytů u pacientů s mnohočetným myelomem

Citovat

OČADLÍKOVÁ, Darina; Lenka ZAHRADOVÁ; Lucie KOVÁŘOVÁ; Miroslav PENKA; Jan ŽALOUDÍK; Roman HÁJEK a Jaroslav MICHÁLEK. Isolation and Expansion of Allogeneic Myeloma-Specific Interferon-Gamma Producing T Cells for Adoptive Immunotherapy. Medical Oncology. Totowa, NJ, USA: Humana Press, 2006, roč. 23/2006, č. 3, s. 377-384. ISSN 1559-131X.

@article{702247,
   author = {Očadlíková, Darina and Zahradová, Lenka and Kovářová, Lucie and Penka, Miroslav and Žaloudík, Jan and Hájek, Roman and Michálek, Jaroslav},
   article_location = {Totowa, NJ, USA},
   article_number = {3},
   keywords = {Multiple myeloma; immunotherapy; interferon-gamma; T cell;CFSE},
   language = {eng},
   issn = {1559-131X},
   journal = {Medical Oncology},
   title = {Isolation and Expansion of Allogeneic Myeloma-Specific Interferon-Gamma Producing T Cells for Adoptive Immunotherapy},
   volume = {23/2006},
   year = {2006}
}

TY  - JOUR
ID  - 702247
AU  - Očadlíková, Darina - Zahradová, Lenka - Kovářová, Lucie - Penka, Miroslav - Žaloudík, Jan - Hájek, Roman - Michálek, Jaroslav
PY  - 2006
TI  - Isolation and Expansion of Allogeneic Myeloma-Specific Interferon-Gamma Producing T Cells for Adoptive Immunotherapy
JF  - Medical Oncology
VL  - 23/2006
IS  - 3
SP  - 377-384
EP  - 377-384
PB  - Humana Press
SN  - 1559131X
KW  - Multiple myeloma
KW  - immunotherapy
KW  - interferon-gamma
KW  - T cell;CFSE
N2  - Adoptive immunotherapy is a promising approach in the treatment of multiple myeloma. We have tested the identification, separation, and expansion of allogeneic myeloma-specific T cells in vitro. Irradiated myeloma cell line ARH 77 has been used to stimulate allogeneic CD4+ and CD8+ T lymphocytes. Activated myeloma-specific T cells that produced interferon-gamma were isolated using immunomagnetic beads and further expanded in vitro to numbers of up to 400 e06 T cells. Specificity of the T lymphocytes was tested using a 5-(6-)carboxyfluoresceine diacetate succinimidyl ester (CFSE)–based cytotoxicity test. This study demonstrates the feasibility of identification and isolation of tumor-specific T cells from allogeneic donors that can be expanded in vitro to numbers useful for clinical applications.
ER  -

OČADLÍKOVÁ, Darina; Lenka ZAHRADOVÁ; Lucie KOVÁŘOVÁ; Miroslav PENKA; Jan ŽALOUDÍK; Roman HÁJEK a Jaroslav MICHÁLEK. Isolation and Expansion of Allogeneic Myeloma-Specific Interferon-Gamma Producing T Cells for Adoptive Immunotherapy. \textit{Medical Oncology}. Totowa, NJ, USA: Humana Press, 2006, roč.~23/2006, č.~3, s.~377-384. ISSN~1559-131X.

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