| HASOŇ, Stanislav a Vladimír VETTERL. Amplified oligonucleotide sensing in microliter volumes containing copper ions by solution streaming. Analytical Chemistry. USA: AMER CHEMICAL SOC, 1155 16TH ST, NW, WAS, 2006, roč. 78/2006, č. 14, s. 5179-5183. ISSN 0003-2700. |
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@article{710547,
author = {Hasoň, Stanislav and Vetterl, Vladimír},
article_location = {USA},
article_number = {14},
keywords = {cathodic stripping voltammetry; graphite electrodes; DNA hybridization; magnetic nanoparticles; oligodeoxynucleotides;},
language = {eng},
issn = {0003-2700},
journal = {Analytical Chemistry},
title = {Amplified oligonucleotide sensing in microliter volumes containing copper ions by solution streaming},
volume = {78/2006},
year = {2006}
}
TY - JOUR
ID - 710547
AU - Hasoň, Stanislav - Vetterl, Vladimír
PY - 2006
TI - Amplified oligonucleotide sensing in microliter volumes containing copper ions by solution streaming
JF - Analytical Chemistry
VL - 78/2006
IS - 14
SP - 5179-5183
EP - 5179-5183
PB - AMER CHEMICAL SOC, 1155 16TH ST, NW, WAS
SN - 00032700
KW - cathodic stripping voltammetry
KW - graphite electrodes
KW - DNA hybridization
KW - magnetic nanoparticles
KW - oligodeoxynucleotides;
N2 - A simple, cost-effective design for amplifying oligodeoxynucleotide (ODN) sensing, in microliter ODN volumes containing copper ions, by solution streaming ( bubbling)is presented. The inert gas streaming ( bubbling) at a constant pressure of 0.04 bar drives the motion of a 30 microliter ODN droplet containing a three-electrode circuit ( inverted drop microcell), and in the presence of copper ions offers an similar to 50-times improvement in the detection of ODN samples. The detection of ODNs at the carbon paste electrode is based on the enhancement of the oxidation peaks of purine bases ( adenine and guanine) by the anodic stripping of the electrochemically accumulated complex of Cu(I) with purine base residues of acid hydrolyzed ODN samples ( Cu(I)-ahODN complex).
ER -
HASOŇ, Stanislav a Vladimír VETTERL. Amplified oligonucleotide sensing in microliter volumes containing copper ions by solution streaming. \textit{Analytical Chemistry}. USA: AMER CHEMICAL SOC, 1155 16TH ST, NW, WAS, 2006, roč.~78/2006, č.~14, s.~5179-5183. ISSN~0003-2700.
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