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@article{714237, author = {Skalníková, Magdalena and Bártová, Eva and Ulman, Vladimír and Matula, Petr and Svoboda, David and Harničarová, Andrea and Kozubek, Michal and Kozubek, Stanislav}, article_location = {Prague}, article_number = {6}, keywords = {Histone methylation; acetylation; X chromosome; centromere; image analysis}, language = {eng}, issn = {0862-8408}, journal = {Physiological Research}, title = {Distinct patterns of histone methylation and acetylation in human interphase nuclei}, volume = {56}, year = {2007} }
TY - JOUR ID - 714237 AU - Skalníková, Magdalena - Bártová, Eva - Ulman, Vladimír - Matula, Petr - Svoboda, David - Harničarová, Andrea - Kozubek, Michal - Kozubek, Stanislav PY - 2007 TI - Distinct patterns of histone methylation and acetylation in human interphase nuclei JF - Physiological Research VL - 56 IS - 6 SP - 797-806 EP - 797-806 PB - Czech Academy of Sciences SN - 08628408 KW - Histone methylation KW - acetylation KW - X chromosome KW - centromere KW - image analysis N2 - To study 3D nuclear distributions of epigenetic histone modifications such as H3(K9) acetylation, H3(K4) dimethylation, H3(K9) dimethylation, and H3(K27) trimethylation, and of histone methyl transferase Suv39H1, we used advanced image analysis methods, combined with Nipkow disk confocal microscopy. Total fluorescence intensity and distributions of fluorescently labelled proteins were analysed in formaldehyde-fixed interphase nuclei. Our data showed reduced fluorescent signals of H3(K9) acetylation and H3(K4) dimethylation (dime) at the nuclear periphery, while di-meH3(K9) was also abundant in chromatin regions closely associated with the nuclear envelope. Little overlapping (intermingling) was observed for di-meH3(K4) and H3(K27) trimethylation (tri-me), and for di-meH3(K9) and Suv39H1. The histone modifications studied were absent in the nucleolar compartment with an exception of H3(K9) dimethylation that was closely associated to perinucleolar regions which are formed by centromeres of acrocentric chromosomes. Using immunocytochemistry, no dimeH3( K4) but just dense di-meH3(K9) was found for the human acrocentric chromosomes 14 and 22. The active X chromosome was observed to be partially acetylated, while the inactive X was more condensed, located in a very peripheral part of the interphase nuclei, and lacked H3(K9) acetylation. Our results confirmed specific interphase patterns of histone modifications within the interphase nuclei as well as within their chromosome territories. ER -
SKALNÍKOVÁ, Magdalena, Eva BÁRTOVÁ, Vladimír ULMAN, Petr MATULA, David SVOBODA, Andrea HARNIČAROVÁ, Michal KOZUBEK a Stanislav KOZUBEK. Distinct patterns of histone methylation and acetylation in human interphase nuclei. \textit{Physiological Research}. Prague: Czech Academy of Sciences, 2007, roč.~56, č.~6, s.~797-806. ISSN~0862-8408.
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