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@article{727109,
author = {Sato, Yukari and Natsume, Rio and Tsuda, M. and Damborský, Jiří and Nagata, Yuji and Senda, T.},
article_number = {4},
keywords = {Haloalkane dehalogenases; degradation of halogenated aliphatic pollutants; DbjA; crystals},
language = {eng},
issn = {1744-3091},
journal = {Acta Crystallographica Section F},
title = {Crystallization and Preliminary Crystallographic Analysis of a Haloalkane Dehalogenase DbjA from Bradyrhizobium japonicum USDA110.},
url = {http://loschmidt.chemi.muni.cz/peg/abstracts/acsf07.html},
volume = {63},
year = {2007}
}
TY - JOUR
ID - 727109
AU - Sato, Yukari - Natsume, Rio - Tsuda, M. - Damborský, Jiří - Nagata, Yuji - Senda, T.
PY - 2007
TI - Crystallization and Preliminary Crystallographic Analysis of a Haloalkane Dehalogenase DbjA from Bradyrhizobium japonicum USDA110.
JF - Acta Crystallographica Section F
VL - 63
IS - 4
SP - 294-296
EP - 294-296
SN - 17443091
KW - Haloalkane dehalogenases
KW - degradation of halogenated aliphatic pollutants
KW - DbjA
KW - crystals
UR - http://loschmidt.chemi.muni.cz/peg/abstracts/acsf07.html
N2 - Haloalkane dehalogenases are key enzymes for the degradation of halogenated aliphatic pollutants. A haloalkane dehalogenase, DbjA, constitutes a novel substrate specificity class with high catalytic activity for beta-methylated haloalkanes. In order to reveal the mechanism of the substrate specificity, DbjA has been crystallized by the hanging drop vapor diffusion method. The best crystals were obtained by the microseeding technique with a reservoir solution of 17-19.5% (w/v) PEG 4000, 0.2 M calcium acetate, and 0.1 M Tris-HCl (pH 7.7-8.0). The space group of the DbjA crystal is P212?2 with unit cell parameters a=212.85 A, b=117.84 A, c=55.80 A. The crystal diffracts to 1.75 A resolution.
ER -
SATO, Yukari, Rio NATSUME, M. TSUDA, Jiří DAMBORSKÝ, Yuji NAGATA a T. SENDA. Crystallization and Preliminary Crystallographic Analysis of a Haloalkane Dehalogenase DbjA from Bradyrhizobium japonicum USDA110. \textit{Acta Crystallographica Section F}. 2007, roč.~63, č.~4, s.~294-296. ISSN~1744-3091.
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