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@article{745052,
author = {Harničarová, Andrea and Kozubek, Stanislav and Pacherník, Jiří and Krejčí, Jana and Bártová, Eva},
article_number = {20},
keywords = {Chromosome structure; c-myc gene; Gene expression; RNA-FISH, Differentiation},
issn = {0014-4827},
journal = {Exp Cell Res.},
title = {Distinct nuclear arrangement of active and inactive c-myc genes in control and differentiated colon carcinoma cells.},
volume = {312},
year = {2006}
}
TY - JOUR
ID - 745052
AU - Harničarová, Andrea - Kozubek, Stanislav - Pacherník, Jiří - Krejčí, Jana - Bártová, Eva
PY - 2006
TI - Distinct nuclear arrangement of active and inactive c-myc genes in control and differentiated colon carcinoma cells.
JF - Exp Cell Res.
VL - 312
IS - 20
PB - Elsevier
SN - 00144827
KW - Chromosome structure
KW - c-myc gene
KW - Gene expression
KW - RNA-FISH, Differentiation
N2 - Using sequential RNA-DNA fluorescence in situ hybridization, the nuclear arrangement of both the active and inactive c-myc gene as well as its transcription was investigated in colon cancer HT-29 cells induced to differentiate into enterocytes. Cytogenetic studies revealed the presence of two chromosomes 8 in HT-29 cells, of which the one containing c-myc gene amplicons was substantially larger and easily distinguished from the normal chromosome. This observation enabled detection of both activity and nuclear localization of c-myc genes in single cells and in individual chromosome territories. Similar transcriptional activity of the c-myc gene was observed in both the normal and derivative chromosome 8 territories showing no influence of the amplification on the c-myc gene expression. Our experiments demonstrate strikingly specific nuclear and territorial arrangements of active genes as compared with inactive ones: on the periphery of their territories facing to the very central region of the cell nucleus. Nuclear arrangement of c-myc genes and transcripts was conserved during cell differentiation and, therefore, independent of the level of differentiation-specific c-myc gene expression. However, after the induction of differentiation, a more internal territorial location was found for the single copy c-myc gene of normal chromosome 8, while amplicons conserved their territorial topography.
ER -
HARNIČAROVÁ, Andrea. Distinct nuclear arrangement of active and inactive c-myc genes in control and differentiated colon carcinoma cells. \textit{Exp Cell Res.}. Elsevier, 2006, roč.~312, č.~20, 4019-4035. ISSN~0014-4827.
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