2007
Tumor-infiltrating cytotoxic T-cells predict outcome in colorectal carcinoma, clinical stadium II.
GARAJOVÁ, Ingrid; Pavel FABIAN; Rudolf NENUTIL; Marek SVOBODA; Ondřej SLABÝ et al.Základní údaje
Originální název
Tumor-infiltrating cytotoxic T-cells predict outcome in colorectal carcinoma, clinical stadium II.
Název česky
TIL-CTL, predikce prežití u pacientů s kolorektálním karcinomem, stadium II.
Název anglicky
Tumor-infiltrating cytotoxic T-cells predict outcome in colorectal carcinoma, clinical stadium II.
Autoři
Vydání
Olomouc, III. dny diagnostické, prediktivní a experimentální onkologie. Sborník příspěvků. od s. 42-42, 1 s. 2007
Nakladatel
solen
Další údaje
Jazyk
čeština
Typ výsledku
Stať ve sborníku
Obor
30200 3.2 Clinical medicine
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Označené pro přenos do RIV
Ne
Organizační jednotka
Lékařská fakulta
ISBN
978-80-244-1824-7
Klíčová slova anglicky
TIL;CTL; outcome;colorectal carcinoma
Štítky
Změněno: 18. 12. 2007 18:32, MUDr. Ingrid Garajová, Ph.D.
V originále
PURPOSE: Simple methods to identify colorectal cancer (CRC) patients in clinical stadium II (T3-4N0M0, Duke B) at high risk of recurrence are needed. Tumor infiltrating lymphocytes /TILs/ are a possible prognostic factor, cytotoxic TILs (CD8+ cells) are generally considered as prognostically favorable, whereas a subgroup of TILs, regulatory T-cells (T-reg, CD4+ CD25+ FOXP3+) are not.We have evaluated the effect of the presence of intraepithelial CD8+ cells on overall survival in patients with CRC in clinical stadium II. METHODS: Formalin-fixed, paraffin embedded tumor samples from 13 patients with CRC in clinical stadium II were evaluated by immunohistochemistry using commercially available anti-CD8 mouse monoclonal antibody. Intraepithelial CD8+ cells were enumerated in one high power magnification field in the area with the highest CD8+ cell infiltration. Its prognostic effect was evaluated using Kaplan-Mayer method. Moreover, pre-operative, total white cells and individual counts of eosinophiles, neutrophiles, lymphocytes were evaluated from peripheral blood as well. RESULTS: We divided the patients into two groups according to the number of intraepithelial CD8+ T-cells, as quantified in the most abundant areas, so called hot spots. First group with 0 to 2 CD8+ cells and the second group with more than 2 CD8+ cells. The median observation period was 50 months. The number of intraepithelial CD8+ T-cells correlated with patients` longer overall survival (poor prognosis for the patients in the first group with less CD8+ cells). CONCLUSION: We conclude that infiltration of CD8+ cells might be an important prognostic factor in CRC in clinical stage II. Knowledge of local immune responses is important for the development of therapeutic strategies. To support our pilot study there are futher experimets undergoing in our laboratory. ACKNOWLEDGMETS: This project is supported by IGA MZ CR NR/9076-4.
Anglicky
PURPOSE: Simple methods to identify colorectal cancer (CRC) patients in clinical stadium II (T3-4N0M0, Duke B) at high risk of recurrence are needed. Tumor infiltrating lymphocytes /TILs/ are a possible prognostic factor, cytotoxic TILs (CD8+ cells) are generally considered as prognostically favorable, whereas a subgroup of TILs, regulatory T-cells (T-reg, CD4+ CD25+ FOXP3+) are not.We have evaluated the effect of the presence of intraepithelial CD8+ cells on overall survival in patients with CRC in clinical stadium II. METHODS: Formalin-fixed, paraffin embedded tumor samples from 13 patients with CRC in clinical stadium II were evaluated by immunohistochemistry using commercially available anti-CD8 mouse monoclonal antibody. Intraepithelial CD8+ cells were enumerated in one high power magnification field in the area with the highest CD8+ cell infiltration. Its prognostic effect was evaluated using Kaplan-Mayer method. Moreover, pre-operative, total white cells and individual counts of eosinophiles, neutrophiles, lymphocytes were evaluated from peripheral blood as well. RESULTS: We divided the patients into two groups according to the number of intraepithelial CD8+ T-cells, as quantified in the most abundant areas, so called hot spots. First group with 0 to 2 CD8+ cells and the second group with more than 2 CD8+ cells. The median observation period was 50 months. The number of intraepithelial CD8+ T-cells correlated with patients` longer overall survival (poor prognosis for the patients in the first group with less CD8+ cells). CONCLUSION: We conclude that infiltration of CD8+ cells might be an important prognostic factor in CRC in clinical stage II. Knowledge of local immune responses is important for the development of therapeutic strategies. To support our pilot study there are futher experimets undergoing in our laboratory. ACKNOWLEDGMETS: This project is supported by IGA MZ CR NR/9076-4.
Návaznosti
| NR9076, projekt VaV |
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