ŠKAREK, Michal, Jaroslav JANOŠEK, Pavel ČUPR, Jiří KOHOUTEK, A. NOVOTNÁ - RYCHETSKÁ a Ivan HOLOUBEK. Evaluation of genotoxic and non-genotoxic effects of organic air pollution using in vitro bioassays. Environment International. roč. 33/7/2007, 33/7/2007, s. 859-866. ISSN 0160-4120. 2007.
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Základní údaje
Originální název Evaluation of genotoxic and non-genotoxic effects of organic air pollution using in vitro bioassays
Název česky Hodnocení genotoxických a negenotoxických efektů organických polutantů volného ovzduší s využitím in vitro testů
Autoři ŠKAREK, Michal (203 Česká republika), Jaroslav JANOŠEK (203 Česká republika), Pavel ČUPR (203 Česká republika, garant), Jiří KOHOUTEK (203 Česká republika), A. NOVOTNÁ - RYCHETSKÁ (203 Česká republika) a Ivan HOLOUBEK (203 Česká republika).
Vydání Environment International, 2007, 0160-4120.
Další údaje
Originální jazyk angličtina
Typ výsledku Článek v odborném periodiku
Obor 10511 Environmental sciences
Stát vydavatele Spojené státy
Utajení není předmětem státního či obchodního tajemství
Impakt faktor Impact factor: 2.797
Kód RIV RIV/00216224:14310/07:00023700
Organizační jednotka Přírodovědecká fakulta
UT WoS 000249677300001
Klíčová slova anglicky Air pollution; PAHs; Genotoxicity; Dioxin toxicity; Bioassay
Štítky air pollution, bioassay, Dioxin toxicity, genotoxicity, PaHs
Příznaky Mezinárodní význam, Recenzováno
Změnil Změnil: doc. RNDr. Pavel Čupr, Ph.D., učo 16144. Změněno: 30. 1. 2008 09:45.
Anotace
In this study, organic extracts of total suspended particles (TSP) and the particulate matter (PM) with the size below 2.5 um (PM2.5) combined with organic extracts of the gas phase (GP) collected at two urban and two background localities were analyzed with a bacterial genotoxicity test, SOS chromotest, and an in vitro test for the dioxin toxicity determination, using a modified cell-line of rat hepatoma H4IIE.luc. In addition, the samples of TSP and GP were analyzed for PAHs contents. The PAHs concentrations and both of the toxic activities at the urban localities were much higher than ones at the background localities. Predominantly, traffic was a source of the urban air pollution there which was also confirmed by the evaluation of portions of certain PAHs (BaP/BPE, PYR/BaP) at the localities. On the other hand, the background localities were apparently affected by a long-distance transport of the pollutants from urban and industrial centers. The results of the bioassays indicated potential health risks for the population exposed to the organic air pollutants, especially at the urban localities. Based on the collected samples, distribution of the organic pollutants with the toxic effects in the air was evaluated. The significant portion of the direct genotoxins was bound to the particles larger than 2.5 um. On the contrary, the indirect genotoxins were bound predominantly to the particles with the size below 2.5 um. However, in the urban air they may be also bound to the larger particles, as well. While the direct genotoxicity may be related with the presence of PAH-derivatives as well as some polar organic pollutants, the indirect genotoxicity is related with the detected carcinogenic PAHs. But besides the above specified pollutants it is also necessary to consider the presence of other toxic components of the complex organic air pollution mixture that may also show potential health risks. This study demonstrates application of the combination of the screening bioassays for the evaluation of organic air pollution and identification of its health risks.
Anotace česky
In this study, organic extracts of total suspended particles (TSP) and the particulate matter (PM) with the size below 2.5 um (PM2.5) combined with organic extracts of the gas phase (GP) collected at two urban and two background localities were analyzed with a bacterial genotoxicity test, SOS chromotest, and an in vitro test for the dioxin toxicity determination, using a modified cell-line of rat hepatoma H4IIE.luc. In addition, the samples of TSP and GP were analyzed for PAHs contents. The PAHs concentrations and both of the toxic activities at the urban localities were much higher than ones at the background localities. Predominantly, traffic was a source of the urban air pollution there which was also confirmed by the evaluation of portions of certain PAHs (BaP/BPE, PYR/BaP) at the localities. On the other hand, the background localities were apparently affected by a long-distance transport of the pollutants from urban and industrial centers. The results of the bioassays indicated potential health risks for the population exposed to the organic air pollutants, especially at the urban localities. Based on the collected samples, distribution of the organic pollutants with the toxic effects in the air was evaluated. The significant portion of the direct genotoxins was bound to the particles larger than 2.5 um. On the contrary, the indirect genotoxins were bound predominantly to the particles with the size below 2.5 um. However, in the urban air they may be also bound to the larger particles, as well. While the direct genotoxicity may be related with the presence of PAH-derivatives as well as some polar organic pollutants, the indirect genotoxicity is related with the detected carcinogenic PAHs. But besides the above specified pollutants it is also necessary to consider the presence of other toxic components of the complex organic air pollution mixture that may also show potential health risks. This study demonstrates application of the combination of the screening bioassays for the evaluation of organic air pollution and identification of its health risks.
Návaznosti
MSM0021622412, záměrNázev: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Akronym: INCHEMBIOL)
Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální , regionální a lokální úrovni
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