Development of IFN-gamma resistance is associated with
attenuation of SOCS genes induction and constitutive expression
of SOCS 3 in melanoma cells

J 2007

Development of IFN-gamma resistance is associated with attenuation of SOCS genes induction and constitutive expression of SOCS 3 in melanoma cells

FOJTOVA, M.; Vladimír BOUDNÝ; A. KOVARIK; L. LAUEROVA; L. ADAMKOVA et al.

Základní údaje

Originální název

Development of IFN-gamma resistance is associated with attenuation of SOCS genes induction and constitutive expression of SOCS 3 in melanoma cells

Název česky

Development of IFN-gamma resistance is associated with attenuation of SOCS genes induction and constitutive expression of SOCS 3 in melanoma cells

Autoři

FOJTOVA, M.; Vladimír BOUDNÝ; A. KOVARIK; L. LAUEROVA; L. ADAMKOVA; K. SOUCKOVA a Jiří JARKOVSKÝ

Vydání

British Journal of Cancer, 2007, 0007-0920

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30200 3.2 Clinical medicine

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 4.635

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/07:00041824

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000248039400012

Klíčová slova anglicky

SOCS proteins; interferon resistance; STAT phosphorylation; malignant melanoma

Štítky

interferon resistance, malignant melanoma, SOCS proteins, STAT phosphorylation

Změněno: 29. 4. 2011 11:02, prof. RNDr. Luděk Bláha, Ph.D.

Anotace

ORIG CZ

V originále

The resistance to interferons (IFNs) limits their anticancer therapeutic efficacy. Here we studied the evolution of an IFN-resistant state in vitro using melanoma cell lines. We found that the cells became less sensitive to antiproliferative effect of IFN-g after prolonged cultivation enabling us to isolate sensitive and resistant subclones of the parental line. We investigated transcription of signal transducer and activator of transcription (STAT) 1 6 and suppressor of cytokine signalling (SOCS) 1 3 genes, and phosphorylation of STAT 1 protein. The resistant subline (termed WM 1158R) differed from the sensitive subline (WM 1158S) by a constitutive expression of SOCS 3, lack or weak SOCS 1 3 activation following IFN g, and short duration of cytokine activatory signal. Similar correlations were observed in additional melanoma lines differing in IFN sensitivities. At the protein level, IFN g induced strong and prolonged STAT 1 activation at serine 727 (S727) in WM 1158R while in WM 1158S cells phosphorylation of this amino acid was much less pronounced.

Česky

Výzkum rozvoje resistence k interferonům, které limitují využitelnost při protinádorové terapii. Studovány byly SOCS proteiny a STAT fosforylace u malignit.

Návaznosti

MSM0021622412, záměr

Název: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Akronym: INCHEMBIOL)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální , regionální a lokální úrovni

Citovat

FOJTOVA, M.; Vladimír BOUDNÝ; A. KOVARIK; L. LAUEROVA; L. ADAMKOVA; K. SOUCKOVA a Jiří JARKOVSKÝ. Development of IFN-gamma resistance is associated with attenuation of SOCS genes induction and constitutive expression of SOCS 3 in melanoma cells. British Journal of Cancer. 2007, roč. 97, č. 2, s. 231-467. ISSN 0007-0920.

@article{753519,
   author = {Fojtova, M. and Boudný, Vladimír and Kovarik, A. and Lauerova, L. and Adamkova, L. and Souckova, K. and Jarkovský, Jiří},
   article_number = {2},
   keywords = {SOCS proteins; interferon resistance; STAT phosphorylation; malignant melanoma},
   language = {eng},
   issn = {0007-0920},
   journal = {British Journal of Cancer},
   title = {Development of IFN-gamma resistance is associated with attenuation of SOCS genes induction and constitutive expression of SOCS 3 in melanoma cells},
   volume = {97},
   year = {2007}
}

TY  - JOUR
ID  - 753519
AU  - Fojtova, M. - Boudný, Vladimír - Kovarik, A. - Lauerova, L. - Adamkova, L. - Souckova, K. - Jarkovský, Jiří
PY  - 2007
TI  - Development of IFN-gamma resistance is associated with attenuation of SOCS genes induction and constitutive expression of SOCS 3 in melanoma cells
JF  - British Journal of Cancer
VL  - 97
IS  - 2
SP  - 231
EP  - 231
SN  - 00070920
KW  - SOCS proteins
KW  - interferon resistance
KW  - STAT phosphorylation
KW  - malignant melanoma
N2  - The resistance to interferons (IFNs) limits their anticancer therapeutic efficacy. Here we studied the evolution of an IFN-resistant state in vitro using melanoma cell lines. We found that the cells became less sensitive to antiproliferative effect of IFN-g after prolonged cultivation enabling us to isolate sensitive and resistant subclones of the parental line. We investigated transcription of signal transducer and activator of transcription (STAT) 1 6 and suppressor of cytokine signalling (SOCS) 1 3 genes, and phosphorylation of STAT 1 protein. The resistant subline (termed WM 1158R) differed from the sensitive subline (WM 1158S) by a constitutive expression of SOCS 3, lack or weak SOCS 1 3 activation following IFN g, and short duration of cytokine activatory signal. Similar correlations were observed in additional melanoma lines differing in IFN sensitivities. At the protein level, IFN g induced strong and prolonged STAT 1 activation at serine 727 (S727) in WM 1158R while in WM 1158S cells phosphorylation of this amino acid was much less pronounced.
ER  -

FOJTOVA, M.; Vladimír BOUDNÝ; A. KOVARIK; L. LAUEROVA; L. ADAMKOVA; K. SOUCKOVA a Jiří JARKOVSKÝ. Development of IFN-gamma resistance is associated with attenuation of SOCS genes induction and constitutive expression of SOCS 3 in melanoma cells. \textit{British Journal of Cancer}. 2007, roč.~97, č.~2, s.~231-467. ISSN~0007-0920.

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