2008
Determination of Asymmetric Dimethylarginine in Human Plasma - Comparison HPLC Methods with Fluorescent or Coulometric Detection with ELISA Method
KLEPÁRNÍK, Martin a Josef TOMANDLZákladní údaje
Originální název
Determination of Asymmetric Dimethylarginine in Human Plasma - Comparison HPLC Methods with Fluorescent or Coulometric Detection with ELISA Method
Název česky
STANOVENÍ ASYMETRICKÉHO DIMETHYLARGININU V LIDSKÉ PLAZMĚ - SROVNÁNÍ HPLC METOD S FLUORESCENČNÍ A COULOMETRICKOU DETEKCÍ S ELISA METODOU
Autoři
KLEPÁRNÍK, Martin a Josef TOMANDL
Vydání
Brno, Sborník příspěvků, od s. 74-75, 117 s. 2008
Nakladatel
Masarykova univerzita
Další údaje
Jazyk
angličtina
Typ výsledku
Stať ve sborníku
Obor
10600 1.6 Biological sciences
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Organizační jednotka
Lékařská fakulta
ISBN
978-80-210-4526-2
Klíčová slova anglicky
ADMA; HPLC; ELISA
Změněno: 3. 12. 2008 20:31, doc. RNDr. Josef Tomandl, Ph.D.
V originále
Asymmetric dimethylarginine (ADMA) is a posttranslationally modified form of arginine that is generated in all types of cells during the process of degradation methylated proteins. The increase of concentration leads to increased blood pressure and reduced blood flow. Elevated plasma ADMA is an independent risk factor for endotelial dysfunction and has been associated with e.g. hypertension, diabetes mellitus, hypercholesterolemia, renal failure and atherosclerosis. In our correlation study we used EDTA-plasma samples from patients 24h after acute myocardial infarction and ADMA concentrations, obtained by HPLC with fluorescent or coulometric detection and ELISA method, were compared and among methods was poor correlation. Detection limits of HPLC methods was approximately 5times lower than in ELISA method and 50times lower than in plasma of healthy people.
Česky
Asymmetric dimethylarginine (ADMA) is a posttranslationally modified form of arginine that is generated in all types of cells during the process of degradation methylated proteins. The increase of concentration leads to increased blood pressure and reduced blood flow. Elevated plasma ADMA is an independent risk factor for endotelial dysfunction and has been associated with e.g. hypertension, diabetes mellitus, hypercholesterolemia, renal failure and atherosclerosis. In our correlation study we used EDTA-plasma samples from patients 24h after acute myocardial infarction and ADMA concentrations, obtained by HPLC with fluorescent or coulometric detection and ELISA method, were compared and among methods was poor correlation. Detection limits of HPLC methods was approximately 5times lower than in ELISA method and 50times lower than in plasma of healthy people.
Návaznosti
| LC06023, projekt VaV |
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| MSM0021622402, záměr |
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