BRINKMAN, Mary Beth, Melanie A. MCGILL, Jonas PETTERSSON, Arthur ROGERS, Petra MATĚJKOVÁ, David ŠMAJS, George M. WEINSTOCK, Steven J. NORRIS and Timothy PALZKILL. A novel Treponema pallidum antigen, TP0136, is an outer membrane protein that binds human fibronectin. INFECTION AND IMMUNITY, The American Society for Microbiology, 2008, vol. 76, No 5, p. 1848-1857. ISSN 0019-9567.
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Basic information
Original name A novel Treponema pallidum antigen, TP0136, is an outer membrane protein that binds human fibronectin.
Name in Czech Nový antigen TP0136 bakterie Treponema pallidum je vnější membránový protein, který váže lidský fibronektin.
Authors BRINKMAN, Mary Beth (840 United States of America), Melanie A. MCGILL (840 United States of America), Jonas PETTERSSON (840 United States of America), Arthur ROGERS (840 United States of America), Petra MATĚJKOVÁ (203 Czech Republic), David ŠMAJS (203 Czech Republic, guarantor), George M. WEINSTOCK (840 United States of America), Steven J. NORRIS (840 United States of America) and Timothy PALZKILL (840 United States of America).
Edition INFECTION AND IMMUNITY, The American Society for Microbiology, 2008, 0019-9567.
Other information
Original language English
Type of outcome article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 3.987
RIV identification code RIV/00216224:14110/08:00024747
Organization unit Faculty of Medicine
UT WoS 000255267200006
Keywords in English Treponema pallidum; protective antigen;
Tags protective antigen, Treponema pallidum
Changed by Changed by: prof. MUDr. David Šmajs, Ph.D., učo 1116. Changed: 25/6/2009 15:47.
Abstract
The antigenicity, structural location, and function of the predicted lipoprotein TP0136 of Treponema pallidum subsp. pallidum were investigated based on previous screening studies indicating that anti-TP0136 antibodies are present in the sera of syphilis patients and experimentally infected rabbits. Recombinant TP0136 (rTP0136) protein was purified and shown to be strongly antigenic during human and experimental rabbit infection. The TP0136 protein was exposed on the surface of the bacterial outer membrane and bound to the host extracellular matrix glycoproteins fibronectin and laminin. In addition, the TP0136 open reading frame was shown to be highly polymorphic among T. pallidum subspecies and strains at the nucleotide and amino acid levels. Finally, the ability of rTP0136 protein to act as a protective antigen to subsequent challenge with infectious T. pallidum in the rabbit model of infection was assessed. Immunization with rTP0136 delayed ulceration but did not prevent infection or the formation of lesions. These results demonstrate that TP0136 is expressed on the outer membrane of the treponeme during infection and may be involved in attachment to host extracellular matrix components.
Abstract (in Czech)
Je popsán nový antigen TP0136 bakterie Treponema pallidum. Jde o vnější membránový protein, který váže lidský fibronektin.
Links
GA310/07/0321, research and development projectName: Komparativní genomové sekvencování patogenních treponem a transkriptomová analýza T. pallidum ssp. pallidum
Investor: Czech Science Foundation, Standard Projects
MSM0021622415, plan (intention)Name: Molekulární podstata buněčných a tkáňových regulací
Investor: Ministry of Education, Youth and Sports of the CR, Research Intents
NR8967, research and development projectName: Molekulární typizace kmenů a izolátů Treponema pallidum subsp. pallidum: základ pro epidemiologickou a klinickou identifikaci jednotlivých kmenů
Investor: Ministry of Health of the CR, Ministry of Agriculture R&D Programme
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