| ŠVEC, Pavel, Dana NOVÁKOVÁ, Lenka ŽÁČKOVÁ, Martina KUKLETOVÁ a Ivo SEDLÁČEK. Evaluation of (GTG)5-PCR for rapid identification of Streptococcus mutans. Online. Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology. Springer, 2008, roč. 94, č. 4, s. 573-579. ISSN 0003-6072. [citováno 2024-04-24] |
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@article{781895,
author = {Švec, Pavel and Nováková, Dana and Žáčková, Lenka and Kukletová, Martina and Sedláček, Ivo},
article_number = {4},
keywords = {Dental caries; (GTG)5-PCR; Identification; Automated ribotyping; Streptococcus mutans},
language = {eng},
issn = {0003-6072},
journal = {Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology},
title = {Evaluation of (GTG)5-PCR for rapid identification of Streptococcus mutans},
url = {http://www.springerlink.com/content/268q817531426227/?p=e909337e76ae416fbddd48f749191788&pi=7},
volume = {94},
year = {2008}
}
TY - JOUR
ID - 781895
AU - Švec, Pavel - Nováková, Dana - Žáčková, Lenka - Kukletová, Martina - Sedláček, Ivo
PY - 2008
TI - Evaluation of (GTG)5-PCR for rapid identification of Streptococcus mutans
JF - Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology
VL - 94
IS - 4
SP - 573-579
EP - 573-579
PB - Springer
SN - 00036072
KW - Dental caries
KW - (GTG)5-PCR
KW - Identification
KW - Automated ribotyping
KW - Streptococcus mutans
UR - http://www.springerlink.com/content/268q817531426227/?p=e909337e76ae416fbddd48f749191788&pi=7
N2 - Repetitive sequence-based polymerase chain reaction (PCR) fingerprinting using the (GTG)5 primer was applied for fast screening of bacterial strains isolated from dental plaque of early childhood caries (ECC)-affected children. A group of 29 Gram-positive bacteria was separated into a homogeneous cluster together with Streptococcus mutans reference strains and constituted an aberrant branch after the numerical analysis of (GTG)5-PCR fingerprints. Automated ribotyping with EcoRI restriction enzyme (RiboPrinter microbial characterization system) revealed high genetic heterogeneity among the tested group and proved to be a good tool for strain-typing purposes. Further characterization of the studied strains was achieved by extensive phenotyping and whole-cell protein fingerprinting and confirmed all the strains as S. mutans representatives. Obtained results showed rep-PCR fingerprinting with the (GTG)5 primer to be a fast and reliable method for identification of S. mutans.
ER -
ŠVEC, Pavel, Dana NOVÁKOVÁ, Lenka ŽÁČKOVÁ, Martina KUKLETOVÁ a Ivo SEDLÁČEK. Evaluation of (GTG)5-PCR for rapid identification of Streptococcus mutans. Online. \textit{Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology}. Springer, 2008, roč.~94, č.~4, s.~573-579. ISSN~0003-6072. [citováno 2024-04-24]
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