Regulation of osteoclast differentiation by the TGFbeta proteins secreted by prostate cancer

VAŇHARA, Petr, Eva LINCOVÁ, Karel SOUČEK a Jan ŠMARDA. Regulation of osteoclast differentiation by the TGFbeta proteins secreted by prostate cancer. In Stem cells, cancer and aging. Singapore: Keystone symposia, International Society of Differentiation, 2008, s. 89-90.

Základní údaje

• Originální název: Regulation of osteoclast differentiation by the TGFbeta proteins secreted by prostate cancer
• Název česky: Regulace osteoklastové diferenciaci proteiny TGFbeta sekretovanymi nadory prostaty
• Autoři: VAŇHARA, Petr, Eva LINCOVÁ, Karel SOUČEK a Jan ŠMARDA.
• Vydání: Singapore, Stem cells, cancer and aging, s. 89-90, 2008.
• Nakladatel: Keystone symposia, International Society of Differentiation

Další údaje

• Originální jazyk: angličtina
• Typ výsledku: Stať ve sborníku
• Obor: Genetika a molekulární biologie
• Stát vydavatele: Singapur
• Utajení: není předmětem státního či obchodního tajemství
• Organizační jednotka: Přírodovědecká fakulta
• Klíčová slova anglicky: TGF; GDF-15; BMP-7; TGFb1; prostate cancer; osteoclast
• Štítky: BMP-7, GDF-15, osteoclast, prostate cancer, TGF, TGFb1
• Příznaky: Mezinárodní význam, Recenzováno

Anotace

Prostate caner is a common disease in western countries. Primary prostate tumors preferentially disseminate to bones. Homing and activity of cancer cells in the bone microenvironment as well as specific mechanisms used by tumor cells remain to be elucidated. This study aims to describe the effects of three members of TGFb protein family (TGFb, BMP-7 and GDF-15) on osteoclast differentiation of murine cell line RAW264.7. These proteins are secreted by prostate cancer cell line LNCaP, possess strong morphogenic effects and are supposed to influence bone microenvironment significantly. TGFb, BMP-7 or GDF-15 affected efficiency of osteoclast differentiation strongly. While TGFb treatment stimulated RANKL-induced differentiation, BMP-7 and GDF-15 cytokines clearly reduced differentiation potential of RAW264.7 cells to active osteoclasts. In contrast to RANKL/TGFb that induced expression of several osteoclast-associated genes, RANKL/GDF-15 and RANKL/BMP-7 suppressed it. According to these we document that different members of the TGFb protein family differently regulate signaling in osteoclasts and consequently modulate the final cellular answer.

Anotace česky

Prostate caner is a common disease in western countries. Primary prostate tumors preferentially disseminate to bones. Homing and activity of cancer cells in the bone microenvironment as well as specific mechanisms used by tumor cells remain to be elucidated. This study aims to describe the effects of three members of TGFb protein family (TGFb, BMP-7 and GDF-15) on osteoclast differentiation of murine cell line RAW264.7. These proteins are secreted by prostate cancer cell line LNCaP, possess strong morphogenic effects and are supposed to influence bone microenvironment significantly. TGFb, BMP-7 or GDF-15 affected efficiency of osteoclast differentiation strongly. While TGFb treatment stimulated RANKL-induced differentiation, BMP-7 and GDF-15 cytokines clearly reduced differentiation potential of RAW264.7 cells to active osteoclasts. In contrast to RANKL/TGFb that induced expression of several osteoclast-associated genes, RANKL/GDF-15 and RANKL/BMP-7 suppressed it. According to these we document that different members of the TGFb protein family differently regulate signaling in osteoclasts and consequently modulate the final cellular answer.

Návaznosti

• GA301/06/0036, projekt VaV: Název: Úloha vybraných transkripčních faktorů v osteoklastové diferenciační dráze

Investor: Grantová agentura ČR, Úloha vybraných transkripčních faktorů v osteoklastové diferenciační dráze

• GD204/08/H054, projekt VaV: Název: Molekulární mechanismy proliferace a diferenciace buněk

Investor: Grantová agentura ČR, Molekulární mechanismy proliferace a diferenciace buněk

• MSM0021622415, záměr: Název: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Molekulární podstata buněčných a tkáňových regulací