RŮŽIČKOVÁ, Vladislava, Pavla HOLOCHOVÁ, Renata KARPÍŠKOVÁ, Roman PANTŮČEK, Markéta POSPÍŠILOVÁ and Jiří DOŠKAŘ. Molecular typing of the enterotoxin H-positive Staphylococcus aureus strains isolated from patients and foods in the Czech Republic. In Pathophysiology of Staphylococci. Abstract book. Würzburg, Germany: Transregional Collaborative Research Center 34, 2008, p. 77.
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Basic information
Original name Molecular typing of the enterotoxin H-positive Staphylococcus aureus strains isolated from patients and foods in the Czech Republic
Name in Czech Molekulární typizace enterotoxin H-pozitivních kmenů Staphylococcus aureus izolovaných z pacientů a potravin v České republice
Authors RŮŽIČKOVÁ, Vladislava (203 Czech Republic, guarantor), Pavla HOLOCHOVÁ (203 Czech Republic), Renata KARPÍŠKOVÁ (203 Czech Republic), Roman PANTŮČEK (203 Czech Republic), Markéta POSPÍŠILOVÁ (203 Czech Republic) and Jiří DOŠKAŘ (203 Czech Republic).
Edition Abstract book. Würzburg, Germany, Pathophysiology of Staphylococci, p. 77-77, 2008.
Publisher Transregional Collaborative Research Center 34
Other information
Original language English
Type of outcome Proceedings paper
Field of Study Genetics and molecular biology
Country of publisher Germany
Confidentiality degree is not subject to a state or trade secret
WWW URL
RIV identification code RIV/00216224:14310/08:00026647
Organization unit Faculty of Science
Keywords in English Staphylococcus aureus; enterotoxin H; staphylococcal food poisoning; molecular diagnostics
Tags enterotoxin H, Molecular diagnostics, Staphylococcal Food Poisoning, Staphylococcus aureus
Tags International impact
Changed by Changed by: prof. RNDr. Roman Pantůček, Ph.D., učo 842. Changed: 31/10/2008 14:52.
Abstract
Staphylococcal enterotoxin H (SEH) is a member of the extracellular mature toxin family, secreted by variety of Staphylococcus aureus strains that were responsible for human disease called Staphylococcal Food Poisoning (SFP). Thirty-two SEH-positive Staphylococcus aureus strains isolated from burned patients and food samples collected in eleven districts of the Czech Republic between 2000 and 2005 were genotypically characterized by detection of the genes for the enterotoxins SEA - SEJ, spa gene polymorphism analysis, pulsed-field gel electrophoresis (PFGE) profiling and ERIC-PCR fingerprinting and prophage carriage. One strain, detected in feta cheese, was implicated in a case of enterotoxinosis. Sixteen of the twenty-eight isolates carried the seh gene alone. The remaining twelve food-derived isolates harbored the seh gene in combination with other enterotoxin genes, most often the seg and sei genes, followed by the sea, seb, sec and sed genes. Four MRSA strains carried the seh and sec and differed from food-derived strains in PFGE, ERIC2-PCR and prophage profiles. Comparison of genomic profiles resulted in the determination of twenty one genotypes designated from G-1 to G-21. Two new, to date not defined, spa types were identified in one strain isolated from raw meat and two strains obtained from prepacked pizza. Our results show that the seh-positive S. aureus isolates from patients and foodstuffs did not originate from a single source or a common ancestor.
Abstract (in Czech)
Staphylococcal enterotoxin H (SEH) is a member of the extracellular mature toxin family, secreted by variety of Staphylococcus aureus strains that were responsible for human disease called Staphylococcal Food Poisoning (SFP). Thirty-two SEH-positive Staphylococcus aureus strains isolated from burned patients and food samples collected in eleven districts of the Czech Republic between 2000 and 2005 were genotypically characterized by detection of the genes for the enterotoxins SEA - SEJ, spa gene polymorphism analysis, pulsed-field gel electrophoresis (PFGE) profiling and ERIC-PCR fingerprinting and prophage carriage. One strain, detected in feta cheese, was implicated in a case of enterotoxinosis. Sixteen of the twenty-eight isolates carried the seh gene alone. The remaining twelve food-derived isolates harbored the seh gene in combination with other enterotoxin genes, most often the seg and sei genes, followed by the sea, seb, sec and sed genes. Four MRSA strains carried the seh and sec and differed from food-derived strains in PFGE, ERIC2-PCR and prophage profiles. Comparison of genomic profiles resulted in the determination of twenty one genotypes designated from G-1 to G-21. Two new, to date not defined, spa types were identified in one strain isolated from raw meat and two strains obtained from prepacked pizza. Our results show that the seh-positive S. aureus isolates from patients and foodstuffs did not originate from a single source or a common ancestor.
Links
MSM0021622415, plan (intention)Name: Molekulární podstata buněčných a tkáňových regulací
Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations
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