2009
Determination of adenosine, inosine and hypoxanthine in human plasma by high performance liquid chromatography
KLEPÁRNÍK, Martin a Josef TOMANDLZákladní údaje
Originální název
Determination of adenosine, inosine and hypoxanthine in human plasma by high performance liquid chromatography
Název česky
Stanovení adenosinu, inosinu a hypoxanthinu v plazmě pomocí HPLC
Autoři
KLEPÁRNÍK, Martin a Josef TOMANDL ORCID
Vydání
Zlín, VITAMINS, NUTRITION, DIAGNOSTICS 2009, The Abstract Book, s. 149-356, 2009
Nakladatel
Univerzita Tomáše Bati ve Zlíně
Další údaje
Jazyk
angličtina
Typ výsledku
Stať ve sborníku
Obor
10406 Analytical chemistry
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Označené pro přenos do RIV
Ne
Organizační jednotka
Lékařská fakulta
ISBN
978-80-7318-809-2
Klíčová slova česky
inosin; hypoxanthin; adenosin; HPLC
Klíčová slova anglicky
inosine; hypoxanthine; adenosine; HPLC
Příznaky
Mezinárodní význam
Změněno: 26. 1. 2010 10:40, doc. RNDr. Josef Tomandl, Ph.D.
V originále
Inosine and hypoxanthine are endogenous low molecular plasma constituents normally found at low concetrations (200-400 ng/mL) in human plasma resulting from dietary and endogenous purine metabolism. Using the mouse model, inosine levels increased from cardiac tissue subjected to cardiac oxidative stress and may serve as potential biomarker indicative of early cardiac ischaemia. We report here development of a new HPLC method for the determination of adenosine, inosine and hypoxanthine using ultra-violet (UV) detection. Before analysis, plasma samples were deproteinized by centrifugal filtration through 10-kDa cut-off membranes. The protein-free ultrafiltrates were analysed on a monolithic reversed phase column Chromolith Performance RP-18e using gradient elution and UV detection at 250nm. Mobile phase consisted of 25 mM acetate buffer and methanol, the flow rate was 1mL/min. Optimal wavelength were confirmed by absorption spectra of inosine and hypoxanthine. Plasma levels of analytes were quantified on the basis of peak area using external standardization.
Česky
Inosine and hypoxanthine are endogenous low molecular plasma constituents normally found at low concetrations (200-400 ng/mL) in human plasma resulting from dietary and endogenous purine metabolism. Using the mouse model, inosine levels increased from cardiac tissue subjected to cardiac oxidative stress and may serve as potential biomarker indicative of early cardiac ischaemia. We report here development of a new HPLC method for the determination of adenosine, inosine and hypoxanthine using ultra-violet (UV) detection. Before analysis, plasma samples were deproteinized by centrifugal filtration through 10-kDa cut-off membranes. The protein-free ultrafiltrates were analysed on a monolithic reversed phase column Chromolith Performance RP-18e using gradient elution and UV detection at 250nm. Mobile phase consisted of 25 mM acetate buffer and methanol, the flow rate was 1mL/min. Optimal wavelength were confirmed by absorption spectra of inosine and hypoxanthine. Plasma levels of analytes were quantified on the basis of peak area using external standardization.
Návaznosti
| LC06023, projekt VaV |
| ||
| MSM0021622402, záměr |
|