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@proceedings{849201,
author = {Hodová, Iveta and Sonnek, Radim and Gelnar, Milan},
booktitle = {6th INTERNATIONAL SYMPOSIUM ON MONOGENEA},
keywords = {SEM; Eudiplozoon nipponicum; Monogenea},
language = {eng},
title = {Different microscopic approaches to research on monogenean parasite Eudiplozoon nipponicum},
year = {2009}
}
TY - CONF
ID - 849201
AU - Hodová, Iveta - Sonnek, Radim - Gelnar, Milan
PY - 2009
TI - Different microscopic approaches to research on monogenean parasite Eudiplozoon nipponicum
KW - SEM
KW - Eudiplozoon nipponicum
KW - Monogenea
N2 - Eudiplozoon nipponicum (Goto, 1891) (Monogenea, Diplozoidae) is a blood-feeding parasite from the gills of carp (Cyprinus carpio L.). A unique feature of the sexual behaviour of monogenean parasites belonging to the family Diplozoidae is that individual adult worms cannot survive alone, two inidviduals fuse together, thereby entering a state of permanent copulation. The scanning electron microscope (SEM) studies of the surface of E. nipponicum have been made to describe tegument and tegumentary structures of all parasite developmental stages: egg, oncomiracidium, unpaired diporpa, paired juvenile and adult parasite specimens. The egg with long filament is smooth and without superficial structures. The non-ciliated surface of the free swimming invasive oncomiracidium has many irregular folds and the ciliated cells are arranged in four zones. The attachment apparatus of unpaired diporpa parasitic the gills of the host fish is not fully developed. Typical structures of this parasite stage are the dorsal papilla and ventral sucker which are responsible for the first contact between diporpae during their pairing. The large mouth of the adult specimen is situated on the ventral side of the forebody. The uniciliated structures that may be sensory structures are found arround the mouth. The tegument of the parasites forebody is highly folded forming transverse ridges. The dorsal and ventral surfaces of the forebody are covered with many ciliated structures. The uterus openings of both partners are situated on the ventral surface in the region of fusion. Transverse tegumentary ridges on the parasite hindbody are highly developed. Running longitudinally along each lateral side of the hindbody, there is a row of papillae-like structures. The SEM observations were complemented by results from the light microscopy, confocal laser scanning microscopy and transmission electron microscopy.
ER -
HODOVÁ, Iveta, Radim SONNEK a Milan GELNAR. Different microscopic approaches to research on monogenean parasite Eudiplozoon nipponicum. In \textit{6th INTERNATIONAL SYMPOSIUM ON MONOGENEA}. 2009.
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